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01-12-2011 | Research

Long-term graft function of cryostored alginate encapsulated rat islets

Authors: Stephan Schneider, Harald H Klein

Published in: European Journal of Medical Research | Issue 9/2011

Abstract

Microencapsulation of pancreatic islets before transplantation is a promising approach to enable graft function in an immunocompetent recipient without immunosuppression. However, the insufficient availability of allogenic islet tissue is a major problem. One concept to overcome these shortcomings is the cryopreservation of encapsulated allogenic islets. Recently, we reported a gentle cryopreservation protocol for rat islets encapsulated in an alginate-based microcapsule system. Here, we report for the first time long-term transplantation data of these cryopreserved microencapsulated islets. We detected a stable graft function for more than 12 month (experiments still continuing) after transplantation of 2500 cryopreserved microencapsulated CD rat islets in streptozotocin-diabetic Wistar rats. Moreover, the glucose clearance rate during an IPGTT was well preserved up to 56 weeks after transplantation. In addition, hyperglycemic blood glucose levels after removal of rat islet grafts 12 and 56 weeks after transplantation confirmed the efficacy of the encapsulated islets. Finally, the retrieved encapsulated rat islets responded well with a 7-fold increase of insulin secretion to a glucose stimulus (12 and 56 weeks). In conclusion, our study demonstrates for the first time that cryopreservation of encapsulated rat islets is possible without substantial losses on graft function for a very long time.

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Title: Long-term graft function of cryostored alginate encapsulated rat islets
Authors: Stephan Schneider
Harald H Klein
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 9/2011
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/2047-783X-16-9-396

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Long-term graft function of cryostored alginate encapsulated rat islets

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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