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Published in: Molecular Autism 1/2013

Open Access 01-12-2013 | Short report

Increased abundance of Sutterella spp. and Ruminococcus torques in feces of children with autism spectrum disorder

Authors: Lv Wang, Claus T Christophersen, Michael J Sorich, Jacobus P Gerber, Manya T Angley, Michael A Conlon

Published in: Molecular Autism | Issue 1/2013

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Abstract

Background

A recent report indicated that numbers of Sutterella spp. are elevated in gastrointestinal biopsies taken from children with autism spectrum disorder (ASD). We have recently reported changes in the numbers of some bacteria within the stool of ASD children, and now examine whether numbers of Sutterella spp. and some other mucosa-associated bacteria linked with gastrointestinal disease (Ruminococcus gnavus and Ruminococcus torques) are also altered in the stool of these children.

Findings

We show that numbers of Sutterella spp. are elevated in feces of ASD children relative to controls, and that numbers of R. torques are higher in the children with ASD with a reported functional gastrointestinal disorder than those without such a disorder.

Conclusions

We show further evidence of changes in the gut microbiota of children with ASD and confirm that the abundance of Sutterella spp. is altered in stool.
Literature
1.
go back to reference Finegold SM, Dowd SE, Gontcharova V, Liu C, Henley KE, Wolcott RD, Youn E, Summanen PH, Granpeesheh D, Dixon D, Liu M, Molitoris DR, Green JA: Pyrosequencing study of fecal microflora of autistic and control children. Anaerobe. 2010, 16: 444-453. 10.1016/j.anaerobe.2010.06.008.CrossRefPubMed Finegold SM, Dowd SE, Gontcharova V, Liu C, Henley KE, Wolcott RD, Youn E, Summanen PH, Granpeesheh D, Dixon D, Liu M, Molitoris DR, Green JA: Pyrosequencing study of fecal microflora of autistic and control children. Anaerobe. 2010, 16: 444-453. 10.1016/j.anaerobe.2010.06.008.CrossRefPubMed
2.
go back to reference Williams BL, Hornig M, Buie T, Bauman ML, Cho Paik M, Wick I, Bennett A, Jabado O, Hirschberg DL, Lipkin WI: Impaired carbohydrate digestion and transport and mucosal dysbiosis in the intestines of children with autism and gastrointestinal disturbances. PLoS One. 2011, 6: e24585-10.1371/journal.pone.0024585.PubMedCentralCrossRefPubMed Williams BL, Hornig M, Buie T, Bauman ML, Cho Paik M, Wick I, Bennett A, Jabado O, Hirschberg DL, Lipkin WI: Impaired carbohydrate digestion and transport and mucosal dysbiosis in the intestines of children with autism and gastrointestinal disturbances. PLoS One. 2011, 6: e24585-10.1371/journal.pone.0024585.PubMedCentralCrossRefPubMed
3.
go back to reference Parracho HM, Bingham MO, Gibson GR, McCartney AL: Differences between the gut microflora of children with autistic spectrum disorders and that of healthy children. J Med Microbiol. 2005, 54: 987-991. 10.1099/jmm.0.46101-0.CrossRefPubMed Parracho HM, Bingham MO, Gibson GR, McCartney AL: Differences between the gut microflora of children with autistic spectrum disorders and that of healthy children. J Med Microbiol. 2005, 54: 987-991. 10.1099/jmm.0.46101-0.CrossRefPubMed
4.
go back to reference Kang DW, Park JG, Ilhan ZE, Wallstrom G, Labaer J, Adams JB, Krajmalnik-Brown R: Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children. PLoS One. 2013, 8: e68322-10.1371/journal.pone.0068322.PubMedCentralCrossRefPubMed Kang DW, Park JG, Ilhan ZE, Wallstrom G, Labaer J, Adams JB, Krajmalnik-Brown R: Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children. PLoS One. 2013, 8: e68322-10.1371/journal.pone.0068322.PubMedCentralCrossRefPubMed
5.
go back to reference Williams BL, Hornig M, Parekh T, Lipkin WI: Application of novel PCR-based methods for detection, quantitation, and phylogenetic characterization of Sutterella species in intestinal biopsy samples from children with autism and gastrointestinal disturbances. mBio. 2012, 3: 1-11. 10.3391/mbi.2012.3.1.01.CrossRef Williams BL, Hornig M, Parekh T, Lipkin WI: Application of novel PCR-based methods for detection, quantitation, and phylogenetic characterization of Sutterella species in intestinal biopsy samples from children with autism and gastrointestinal disturbances. mBio. 2012, 3: 1-11. 10.3391/mbi.2012.3.1.01.CrossRef
6.
go back to reference Sakon H, Nagai F, Morotomi M, Tanaka R: Sutterella parvirubra sp. nov. and Megamonas funiformis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2008, 58: 970-975. 10.1099/ijs.0.65456-0.CrossRefPubMed Sakon H, Nagai F, Morotomi M, Tanaka R: Sutterella parvirubra sp. nov. and Megamonas funiformis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2008, 58: 970-975. 10.1099/ijs.0.65456-0.CrossRefPubMed
7.
go back to reference Greetham HL, Collins MD, Gibson GR, Giffard C, Falsen E, Lawson PA: Sutterella stercoricanis sp. nov., isolated from canine faeces. Int J Syst Evol Microbiol. 2004, 54: 1581-1584. 10.1099/ijs.0.63098-0.CrossRefPubMed Greetham HL, Collins MD, Gibson GR, Giffard C, Falsen E, Lawson PA: Sutterella stercoricanis sp. nov., isolated from canine faeces. Int J Syst Evol Microbiol. 2004, 54: 1581-1584. 10.1099/ijs.0.63098-0.CrossRefPubMed
8.
go back to reference Molitoris E, Wexler HM, Finegold SM: Sources and antimicrobial susceptibilities of Campylobacter gracilis and Sutterella wadsworthensis. Clin Infect Dis. 1997, 25 (Suppl 2): S264-S265.CrossRefPubMed Molitoris E, Wexler HM, Finegold SM: Sources and antimicrobial susceptibilities of Campylobacter gracilis and Sutterella wadsworthensis. Clin Infect Dis. 1997, 25 (Suppl 2): S264-S265.CrossRefPubMed
9.
go back to reference Engberg J, On SL, Harrington CS, Gerner-Smidt P: Prevalence of campylobacter, arcobacter, helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. J Clin Microbiol. 2000, 38: 286-291.PubMedCentralPubMed Engberg J, On SL, Harrington CS, Gerner-Smidt P: Prevalence of campylobacter, arcobacter, helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. J Clin Microbiol. 2000, 38: 286-291.PubMedCentralPubMed
10.
go back to reference Mukhopadhya I, Hansen R, Nicholl CE, Alhaidan YA, Thomson JM, Berry SH, Pattinson C, Stead DA, Russell RK, El-Omar EM, Hold GL: A comprehensive evaluation of colonic mucosal isolates of Sutterella wadsworthensis from inflammatory bowel disease. PLoS One. 2011, 6: e27076-10.1371/journal.pone.0027076.PubMedCentralCrossRefPubMed Mukhopadhya I, Hansen R, Nicholl CE, Alhaidan YA, Thomson JM, Berry SH, Pattinson C, Stead DA, Russell RK, El-Omar EM, Hold GL: A comprehensive evaluation of colonic mucosal isolates of Sutterella wadsworthensis from inflammatory bowel disease. PLoS One. 2011, 6: e27076-10.1371/journal.pone.0027076.PubMedCentralCrossRefPubMed
11.
go back to reference Wang L, Christophersen CT, Sorich MJ, Gerber JP, Angley MT, Conlon MA: Low relative abundances of the mucolytic bacterium Akkermansia muciniphila and Bifidobacterium spp. in feces of children with autism. Appl Environ Microbiol. 2011, 77: 6718-6721. 10.1128/AEM.05212-11.PubMedCentralCrossRefPubMed Wang L, Christophersen CT, Sorich MJ, Gerber JP, Angley MT, Conlon MA: Low relative abundances of the mucolytic bacterium Akkermansia muciniphila and Bifidobacterium spp. in feces of children with autism. Appl Environ Microbiol. 2011, 77: 6718-6721. 10.1128/AEM.05212-11.PubMedCentralCrossRefPubMed
12.
go back to reference Png CW, Lindén SK, Gilshenan KS, Zoetendal EG, McSweeney CS, Sly LI, McGuckin MA, Florin THJ: Mucolytic bacteria with increased prevalence in IBD mucosa augment in vitro utilization of mucin by other bacteria. Am J Gastroenterol. 2010, 105: 2420-2428. 10.1038/ajg.2010.281.CrossRefPubMed Png CW, Lindén SK, Gilshenan KS, Zoetendal EG, McSweeney CS, Sly LI, McGuckin MA, Florin THJ: Mucolytic bacteria with increased prevalence in IBD mucosa augment in vitro utilization of mucin by other bacteria. Am J Gastroenterol. 2010, 105: 2420-2428. 10.1038/ajg.2010.281.CrossRefPubMed
13.
go back to reference Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J: qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol. 2007, 8: R19-10.1186/gb-2007-8-2-r19.PubMedCentralCrossRefPubMed Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J: qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol. 2007, 8: R19-10.1186/gb-2007-8-2-r19.PubMedCentralCrossRefPubMed
14.
go back to reference Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3: RESEARCH0034.PubMedCentralCrossRefPubMed Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3: RESEARCH0034.PubMedCentralCrossRefPubMed
15.
go back to reference Hoskins LC, Agustines M, McKee WB, Boulding ET, Kriaris M, Niedermeyer G: Mucin degradation in human colon ecosystems. Isolation and properties of fecal strains that degrade ABH blood group antigens and oligosaccharides from mucin glycoproteins. J Clin Invest. 1985, 75: 944-953. 10.1172/JCI111795.PubMedCentralCrossRefPubMed Hoskins LC, Agustines M, McKee WB, Boulding ET, Kriaris M, Niedermeyer G: Mucin degradation in human colon ecosystems. Isolation and properties of fecal strains that degrade ABH blood group antigens and oligosaccharides from mucin glycoproteins. J Clin Invest. 1985, 75: 944-953. 10.1172/JCI111795.PubMedCentralCrossRefPubMed
16.
go back to reference Dethlefsen L, Eckburg PB, Bik EM, Relman DA: Assembly of the human intestinal microbiota. Trends Ecol Evol. 2006, 21: 517-523. 10.1016/j.tree.2006.06.013.CrossRefPubMed Dethlefsen L, Eckburg PB, Bik EM, Relman DA: Assembly of the human intestinal microbiota. Trends Ecol Evol. 2006, 21: 517-523. 10.1016/j.tree.2006.06.013.CrossRefPubMed
17.
go back to reference Joossens M, Huys G, Cnockaert M, De Preter V, Verbeke K, Rutgeerts P, Vandamme P, Vermeire S: Dysbiosis of the faecal microbiota in patients with Crohn’s disease and their unaffected relatives. Gut. 2011, 60: 631-637. 10.1136/gut.2010.223263.CrossRefPubMed Joossens M, Huys G, Cnockaert M, De Preter V, Verbeke K, Rutgeerts P, Vandamme P, Vermeire S: Dysbiosis of the faecal microbiota in patients with Crohn’s disease and their unaffected relatives. Gut. 2011, 60: 631-637. 10.1136/gut.2010.223263.CrossRefPubMed
18.
go back to reference de Magistris L, Familiari V, Pascotto A, Sapone A, Frolli A, Iardino P, Carteni M, De Rosa M, Francavilla R, Riegler G, Militerni R, Bravaccio C: Alterations of the intestinal barrier in patients with autism spectrum disorders and in their first-degree relatives. J Pediatr Gastr Nutr. 2010, 51: 418-424. 10.1097/MPG.0b013e3181dcc4a5.CrossRef de Magistris L, Familiari V, Pascotto A, Sapone A, Frolli A, Iardino P, Carteni M, De Rosa M, Francavilla R, Riegler G, Militerni R, Bravaccio C: Alterations of the intestinal barrier in patients with autism spectrum disorders and in their first-degree relatives. J Pediatr Gastr Nutr. 2010, 51: 418-424. 10.1097/MPG.0b013e3181dcc4a5.CrossRef
19.
go back to reference D’Eufemia P, Celli M, Finocchiaro R, Pacifico L, Viozzi L, Zaccagnini M, Cardi E, Giardini O: Abnormal intestinal permeability in children with autism. Acta Paediatr. 1996, 85: 1076-1079. 10.1111/j.1651-2227.1996.tb14220.x.CrossRefPubMed D’Eufemia P, Celli M, Finocchiaro R, Pacifico L, Viozzi L, Zaccagnini M, Cardi E, Giardini O: Abnormal intestinal permeability in children with autism. Acta Paediatr. 1996, 85: 1076-1079. 10.1111/j.1651-2227.1996.tb14220.x.CrossRefPubMed
Metadata
Title
Increased abundance of Sutterella spp. and Ruminococcus torques in feces of children with autism spectrum disorder
Authors
Lv Wang
Claus T Christophersen
Michael J Sorich
Jacobus P Gerber
Manya T Angley
Michael A Conlon
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Molecular Autism / Issue 1/2013
Electronic ISSN: 2040-2392
DOI
https://doi.org/10.1186/2040-2392-4-42

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