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Open Access 01-12-2014 | Brief communication

New construction of an animal model for the orthotopic transplantation of an ovarian tumor

Authors: Hui Zhang, Xinping Gao, Yongan Yang, Weiming Wang, Jin Liu, Yijuan Liang, Hongli Wu, Jinjin Qin, Kun Pan, Yifeng Wang, Junrong Shi, Youju Ma

Published in: Journal of Ovarian Research | Issue 1/2014

Abstract

A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2K^d/H-2D^d) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.

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Title: New construction of an animal model for the orthotopic transplantation of an ovarian tumor
Authors: Hui Zhang
Xinping Gao
Yongan Yang
Weiming Wang
Jin Liu
Yijuan Liang
Hongli Wu
Jinjin Qin
Kun Pan
Yifeng Wang
Junrong Shi
Youju Ma
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2014
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/1757-2215-7-64

At a glance: The STEP trials

Springer Medicine

New construction of an animal model for the orthotopic transplantation of an ovarian tumor

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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