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Open Access 01-12-2014 | Research

Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

Authors: Luciana Lamarão Damous, Juliana Sanajotti Nakamuta, José Maria Soares-Jr, Gustavo Arantes Rosa Maciel, Ricardo dos Santos Simões, Edna Frasson de Souza Montero, José Eduardo Krieger, Edmund Chada Baracat

Published in: Journal of Ovarian Research | Issue 1/2014

Abstract

Background

Cryopreservation of the ovarian tissue has shown promising results. However, there remain controversial issues such as the short half-life of grafts. In this aspect, there are some evidences that preconditioning the ovarian tissue before transplantation is beneficial.

Objective

To determine the effect of hypoxic preconditioning in vitro on ovarian tissue prior to transplantation.

Methods

Eighteen female adult Wistar rats, were sorted into three experimental groups. Ovaries were maintained in DMEM low glucose serum free at 37°C with 5% CO2, at atmospheric oxigen concentration (normoxia) or 1% O₂ (hypoxia) for 16 hours. Oxigen concentration was determined by injection of nitrogen in the incubator. Animals submitted to ovarian transplantation immediately after oophorectomy were the Control Group (C). After this, the ovaries were implanted in the retroperitoneum with nonabsorbable suture and animals evaluated for thirty days after transplantation. Beginning on postoperative (PO) day 11, a daily collection of vaginal smear was carried out. Analyses comprised morphological, morphometric (counting ovarian follicles and corpora lutea) and immunohistochemistry for cleaved caspase-3 (apoptosis).

Results

In normoxia and control groups all animals recovered their estrous cycles, while in the hypoxia group, two animals did not ovulate but, among those which did, resumption took longer than in the other groups (p < 0.05). The number of ovarian follicles and corpora lutea decreased significantly in the hypoxia group when compared to the other two groups (p < 0.001) and apoptosis was increased in the few ovarian follicles which remained viable (p < 0.001).

Conclusion

The hypoxic preconditioning in vitro was not beneficial to the graft and worsened their viability, compromising its functionality or delaying the return of this.

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Title: Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function
Authors: Luciana Lamarão Damous
Juliana Sanajotti Nakamuta
José Maria Soares-Jr
Gustavo Arantes Rosa Maciel
Ricardo dos Santos Simões
Edna Frasson de Souza Montero
José Eduardo Krieger
Edmund Chada Baracat
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2014
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/1757-2215-7-34

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function

Abstract

Background

Objective

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Objective

Methods

Results

Conclusion

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