Top

Journal of Ovarian Research

Published in:

Open Access 01-12-2013 | Research

Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation

Authors: Lisa Rooper, Hilal Gurler, Andre A Kajdacsy-Balla, Maria V Barbolina

Published in: Journal of Ovarian Research | Issue 1/2013

Abstract

Background

The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX₃CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation.

Methods

Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX₃CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation.

Results

We found that the majority of the specimens contained CX₃CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX₃CR1- positive. PA-1 cells with downregulated CX₃CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA.

Conclusions

Here we demonstrate that CX₃CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.

Available only for authorised users

Prat J: Female reproductive system. In Anderson’s pathology. Edited by: Damjanov ILJ. St. Louis: Mosby; 1996:2231–2309.

Gasim T, Al Dakhiel SA, Al Ghamdi AA, Al Ali M, Al Jama F, Rahman J, Al Suleiman SA, Rahman MS: Ovarian tumors associated with pregnancy: a 20-year experience in a teaching hospital. Arch Gynecol Obstet 2010,282(5):529–533. 10.1007/s00404-009-1346-zPubMedCrossRef

Fishman A, Edelstein E, Altaras M, Beyth Y, Bernheim J: Adenocarcinoma arising from the gastrointestinal epithelium in benign cystic teratoma of the ovary. Gynecol Oncol 1998,70(3):418–420. 10.1006/gyno.1998.5123PubMedCrossRef

Kido A, Togashi K, Konishi I, Kataoka ML, Koyama T, Ueda H, Fujii S, Konishi J: Dermoid cysts of the ovary with malignant transformation: MR appearance. AJR Am J Roentgenol 1999,172(2):445–449. 10.2214/ajr.172.2.9930800PubMedCrossRef

Parrington JM, West LF, Povey S: The origin of ovarian teratomas. J Med Genet 1984,21(1):4–12. 10.1136/jmg.21.1.4PubMedCentralPubMedCrossRef

Outwater EK, Siegelman ES, Hunt JL: Ovarian teratomas: tumor types and imaging characteristics. Radiographics 2001,21(2):475–490.PubMedCrossRef

Rha SE, Byun JY, Jung SE, Kim HL, Oh SN, Kim H, Lee H, Kim BK, Lee JM: Atypical CT and MRI manifestations of mature ovarian cystic teratomas. AJR Am J Roentgenol 2004,183(3):743–750. 10.2214/ajr.183.3.1830743PubMedCrossRef

Kim M, Rooper L, Xie J, Kajdacsy-Balla AA, Barbolina MV: Fractalkine receptor CX(3)CR1 is expressed in epithelial ovarian carcinoma cells and required for motility and adhesion to peritoneal mesothelial cells. Mol Cancer Res 2012,10(1):11–24. 10.1158/1541-7786.MCR-11-0256PubMedCentralPubMedCrossRef

D’Haese JG, Demir IE, Friess H, Ceyhan GO: Fractalkine/CX3CR1: why a single chemokine-receptor duo bears a major and unique therapeutic potential. Expert Opin Ther Targets 2010,14(2):207–219. 10.1517/14728220903540265PubMedCrossRef

10.

D’Haese JG, Friess H, Ceyhan GO: Therapeutic potential of the chemokine-receptor duo fractalkine/CX3CR1: an update. Expert Opin Ther Targets 2012,16(6):613–618. 10.1517/14728222.2012.682574PubMedCrossRef

11.

Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki M, Takagi S, Nomiyama H, Schall TJ, Yoshie O: Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 1997,91(4):521–530. 10.1016/S0092-8674(00)80438-9PubMedCrossRef

12.

Koizumi K, Saitoh Y, Minami T, Takeno N, Tsuneyama K, Miyahara T, Nakayama T, Sakurai H, Takano Y, Nishimura M, Imai T, Yoshie O, Saiki I: Role of CX3CL1/fractalkine in osteoclast differentiation and bone resorption. J Immunol 2009,183(12):7825–7831. 10.4049/jimmunol.0803627PubMedCrossRef

13.

Łyszkiewicz M, Witzlau K, Pommerencke J, Krueger A: Chemokine receptor CX3CR1 promotes dendritic cell development under steady-state conditions. Eur J Immunol 2012,41(5):1256–1265.CrossRef

14.

Barbolina MV, Kim M, Liu Y, Shepard J, Belmadani A, Miller RJ, Shea LD, Stack MS: Microenvironmental regulation of chemokine (C-X-C-motif) receptor 4 in ovarian carcinoma. Mol Cancer Res 2010,8(5):653–664. 10.1158/1541-7786.MCR-09-0463PubMedCentralPubMedCrossRef

15.

Kim M, Rooper L, Xie J, Rayahin J, Burdette JE, Kajdacsy-Balla AA, Barbolina MV: The lymphotactin receptor is expressed in epithelial ovarian carcinoma and contributes to cell migration and proliferation. Mol Cancer Res 2012,10(11):1419–1429. 10.1158/1541-7786.MCR-12-0361PubMedCrossRef

16.

Giovanella BC, Stehlin JS, Williams LJ Jr: Heterotransplantation of human malignant tumors in “nude” thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J Natl Cancer Inst 1974,52(3):921–930.PubMed

17.

Zeuthen J, Nørgaard JO, Avner P, Fellous M, Wartiovaara J, Vaheri A, Rosén A, Giovanella BC: Characterization of a human ovarian teratocarcinoma-derived cell line. Int J Cancer 1980,25(1):19–32. 10.1002/ijc.2910250104PubMedCrossRef

18.

Xia SL, Zhang X, Jing NH: The induction and differentiation of a human teratocarcinoma cell line (PA-1) in vitro]. Shi yan sheng wu xue bao 1995,28(4):397–407.PubMed

19.

Zeuthen J: Human teratocarcinoma cell lines. A review. Workshop on early detection of testicular cancer. Copenhagen, Denmark. Int J Androl 1981, Supplementum 4: 61–77.CrossRef

20.

Illmensee K, Mintz B: Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts. Proc Natl Acad Sci USA 1976,73(2):549–553. 10.1073/pnas.73.2.549PubMedCentralPubMedCrossRef

21.

Saba L, Guerriero S, Sulcis R, Virgilio B, Melis G, Mallarini G: Mature and immature ovarian teratomas: CT, US and MR imaging characteristics. Eur J Radiol 2009,72(3):454–463. 10.1016/j.ejrad.2008.07.044PubMedCrossRef

22.

Ishida Y, Gao JL, Murphy PM: Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function. J Immunol 2008,180(1):569–579.PubMedCrossRef

Title: Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation
Authors: Lisa Rooper
Hilal Gurler
Andre A Kajdacsy-Balla
Maria V Barbolina
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2013
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/1757-2215-6-57

At a glance: The STEP trials

Springer Medicine

Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2013

Antisense oligonucleotide suppression of human IGF-1R inhibits the growth and survival of in vitro cultured epithelial ovarian cancer cells

Ovarian stem cells: absence of evidence is not evidence of absence

Journal of Ovarian Research reviewer acknowledgement 2012

Pronuclear morphology evaluation for fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles: a systematic review

Molecular cytogenetics as a clinical test for prognostic and predictive biomarkers in newly diagnosed ovarian cancer

Mosaic pregnancy after transfer of a “euploid” blastocyst screened by DNA microarray