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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2009

Open Access 01-01-2009 | Research

Effect of sirolimus on urinary bladder cancer T24 cell line

Authors: Rosario Pinto-Leite, Pedro Botelho, Eufemia Ribeiro, Paula A Oliveira, Lucios Santos

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2009

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Abstract

Background

Sirolimus is recently reported to have antitumour effects on a large variety of cancers. The present study was performed to investigate sirolimus's ability to inhibit growth in T24 bladder cancer cells.

Methods

T24 bladder cancer cells were treated with various concentrations of sirolimus. MTT assay was used to evaluate the proliferation inhibitory effect on T24 cell line. The viability of T24 cell line was determined by Trypan blue exclusion analysis.

Results

Sirolimus inhibits the growth of bladder carcinoma cells and decreases their viability. Significant correlations were found between cell proliferation and sirolimus concentration (r = 0.830; p < 0.01) as well as between cell viability and sirolimus concentration (r = -0.896; p < 0.01).

Conclusion

Sirolimus has an anti-proliferation effect on the T24 bladder carcinoma cell line. The information from our results is useful for a better understanding sirolimus's anti-proliferative activity in the T24 bladder cancer cell line.
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Metadata
Title
Effect of sirolimus on urinary bladder cancer T24 cell line
Authors
Rosario Pinto-Leite
Pedro Botelho
Eufemia Ribeiro
Paula A Oliveira
Lucios Santos
Publication date
01-01-2009
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2009
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/1756-9966-28-3

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