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Open Access 01-12-2011 | Short report

Gene expression profiles in BCL11B-siRNA treated malignant T cells

Authors: Xin Huang, Qi Shen, Si Chen, Shaohua Chen, Lijian Yang, Jianyu Weng, Xin Du, Piotr Grabarczyk, Grzegorz K Przybylski, Christian A Schmidt, Yangqiu Li

Published in: Journal of Hematology & Oncology | Issue 1/2011

Abstract

Background

Downregulation of the B-cell chronic lymphocytic leukemia (CLL)/lymphoma11B (BCL11B) gene by small interfering RNA (siRNA) leads to growth inhibition and apoptosis of the human T-cell acute lymphoblastic leukemia (T-ALL) cell line Molt-4. To further characterize the molecular mechanism, a global gene expression profile of BCL11B-siRNA -treated Molt-4 cells was established. The expression profiles of several genes were further validated in the BCL11B-siRNA -treated Molt-4 cells and primary T-ALL cells.

Results

142 genes were found to be upregulated and 109 genes downregulated in the BCL11B-siRNA -treated Molt-4 cells by microarray analysis. Among apoptosis-related genes, three pro-apoptotic genes, TNFSF 10, BIK, BNIP 3, were upregulated and one anti-apoptotic gene, BCL2L 1 was downregulated. Moreover, the expression of SPP 1 and CREBBP genes involved in the transforming growth factor (TGF-β) pathway was down 16-fold. Expression levels of TNFSF 10, BCL2L 1, SPP 1, and CREBBP were also examined by real-time PCR. A similar expression pattern of TNFSF 10, BCL2L 1, and SPP 1 was identified. However, CREBBP was not downregulated in the BLC11B-siRNA -treated Molt-4 cells.

Conclusion

BCL11B-siRNA treatment altered expression profiles of TNFSF 10, BCL2L 1, and SPP 1 in both Molt-4 T cell line and primary T-ALL cells.

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Title: Gene expression profiles in BCL11B-siRNA treated malignant T cells
Authors: Xin Huang
Qi Shen
Si Chen
Shaohua Chen
Lijian Yang
Jianyu Weng
Xin Du
Piotr Grabarczyk
Grzegorz K Przybylski
Christian A Schmidt
Yangqiu Li
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Journal of Hematology & Oncology / Issue 1/2011
Electronic ISSN: 1756-8722
DOI: https://doi.org/10.1186/1756-8722-4-23

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