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Molecular Brain
Published in: Molecular Brain 1/2011

Open Access 01-12-2011 | Research

Genome-wide screen for modifiers of Parkinson's disease genes in Drosophila

Authors: Caroline Fernandes, Yong Rao

Published in: Molecular Brain | Issue 1/2011

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Abstract

Background

Mutations in parkin and PTEN-induced kinase 1 (Pink1) lead to autosomal recessive forms of Parkinson's disease (PD). parkin and Pink1 encode a ubiquitin-protein ligase and a mitochondrially localized serine/threonine kinase, respectively. Recent studies have implicated Parkin and Pink1 in a common and evolutionarily conserved pathway for protecting mitochondrial integrity.

Results

To systematically identify novel components of the PD pathways, we generated a genetic background that allowed us to perform a genome-wide F1 screen for modifiers of Drosophila parkin (park) and Pink1 mutant phenotype. From screening ~80% of the fly genome, we identified a number of cytological regions that interact with park and/or Pink1. Among them, four cytological regions were selected for identifying corresponding PD-interacting genes. By analyzing smaller deficiency chromosomes, available transgenic RNAi lines, and P-element insertions, we identified five PD-interacting genes. Among them, opa1 and drp1 have been previously implicated in the PD pathways, whereas debra (dbr), Pi3K21B and β4GalNAcTA are novel PD-interacting genes.

Conclusions

We took an unbiased genetic approach to systematically isolate modifiers of PD genes in Drosophila. Further study of novel PD-interacting genes will shed new light on the function of PD genes and help in the development of new therapeutic strategies for treating Parkinson's disease.
Appendix
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Metadata
Title
Genome-wide screen for modifiers of Parkinson's disease genes in Drosophila
Authors
Caroline Fernandes
Yong Rao
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Molecular Brain / Issue 1/2011
Electronic ISSN: 1756-6606
DOI
https://doi.org/10.1186/1756-6606-4-17

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