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Chinese Medicine
Published in: Chinese Medicine 1/2014

Open Access 01-12-2014 | Research

Anti-infective and cytotoxic properties of Bupleurum marginatum

Authors: Mohamed L Ashour, Mahmoud Z El-Readi, Razan Hamoud, Safaa Y Eid, Sherweit H El Ahmady, Endalkachew Nibret, Florian Herrmann, Mahmoud Youns, Ahmed Tahrani, Dorothea Kaufmann, Michael Wink

Published in: Chinese Medicine | Issue 1/2014

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Abstract

Background

Bupleurum marginatum Wall. ex DC (Apiaceae) is a perennial herb widely used in traditional Chinese and Kampo medicine for the treatment of various infectious diseases. The biological activities of B. marginatum have not been fully investigated. This study aims to investigate the antitrypanosomal, antimicrobial and antiviral activities of methanol (ME) and dichloromethane (DCM) extracts of B. marginatum aerial parts and the ability of both extracts to inhibit the growth of different cancer cell lines.

Methods

Phytochemical characterization of the extracts was performed by LC-MS profiling. The antitrypanosomal activity was evaluated using the resazurin method. The antimicrobial activity was assessed using agar diffusion and microdilution methods, and the minimum inhibitory concentration (MIC) values were determined. The antiviral activity was determined for 6.25, 12.5, and 50 μg/mL doses using a plaque reduction assay. Cytotoxicity was investigated in eight cancer cell lines (Caco-2, CCL-81, CCRF-CEM, COS-7, HL-60, MIA PaCa-2, MCF-7, and PANC-1) using the MTT assay and the caspase 3/7 activity was determined over the range of 62.5–1000 μg/mL.

Results

Phytochemical analyses resulted in the characterization of 15 components, mainly flavonoids and lignans. The DCM extract showed significant antitrypanosomal activity (IC50: 36.21 μg/mL) and moderate activity against Streptococcus pyogenes (MIC value: 0.25 mg/mL). At a dose of 12.5 μg/mL, the DCM extract inhibited 73.6% of the plaque production by hepatitis A virus. CCRF-CEM cells were the most sensitive to both extracts (IC50: 12.5–22.7 μg/mL). The cytotoxicity was mediated by induction of apoptosis (19-fold increase in the cellular caspase 3/7 level after treatment with the DCM extract at 1 mg/mL).

Conclusions

ME and DCM extract of B. marginatum showed anti-infective and antiproliferative effects.
Appendix
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Literature
1.
Cordell GA: Natural products in drug discovery – Creating a new vision. Phytochem Rev. 2002, 1: 261-273. 10.1023/A:1026094701495.CrossRef
2.
Yaniv Z, Bachrach U: Handbook of medicinal plants. 2005, New York: Food Products Press : Haworth Medical Press
3.
Mabberley DJ: Mabberley's plant-book: a portable dictionary of plants, their classification and uses. 3rd edn. Cambridge, UK. 2008, New York: Cambridge University Press
4.
Pan S-L: Bupleurum species: scientific evaluation and clinical applications. 2006, Boca Raton: CRC/Taylor & FrancisCrossRef
5.
World Health Organization: WHO Monographs on Selected Medicinal Plants Vol 1. 1999, Geneva: World Health Organization
6.
Ashour ML, Wink M: Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action. J Pharm Pharmacol. 2011, 63: 305-321. 10.1111/j.2042-7158.2010.01170.x.CrossRefPubMed
7.
Wu J-N: An illustrated Chinese materia medica. 2005, New York: Oxford University Press
8.
Fundukian LJ: The Gale encyclopedia of alternative medicine. 2009, Gale, Cengage Learning: Detroit, 3
9.
Zhou J: Encyclopedia of traditional Chinese medicines vol 1 - molecular structures, pharmacological activities, natural sources and applications. 2011, New York: SpringerCrossRef
10.
Huang HQ, Zhang X, Lin M, Shen YH, Yan SK, Zhang WD: Characterization and identification of saikosaponins in crude extracts from three Bupleurum species using LC-ESI-MS. J Sep Sci. 2008, 31: 3190-3201. 10.1002/jssc.200800120.CrossRefPubMed
11.
Ashour ML, El-Readi MZ, Tahrani A, Eid SY, Wink M: A novel cytotoxic aryltetraline lactone from Bupleurum marginatum (Apiaceae). Phytochem Lett. 2012, 5: 387-392. 10.1016/j.phytol.2012.03.009.CrossRef
12.
Zhitao L, Minjian Q, Zhengtao W: Study on the constituent s of the roots of Bupleurum Marginatum. J China Pharmaceut Uni. 2003, 34: 305-308. [In Chinese]
13.
Liu Y, Zhang T-T, Zhou J-S, Wang Q: Three new arylnaphthalide lignans from the aerial parts of Bupleurum marginatum WALL. ex DC. Helv Chim Acta. 2008, 91: 2316-2320. 10.1002/hlca.200890252.CrossRef
14.
Ashour ML, El-Readi M, Youns M, Mulyaningsih S, Sporer F, Efferth T, Wink M: Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae). J Pharm Pharmacol. 2009, 61: 1079-1087.CrossRefPubMed
15.
Hamdan D, El-Readi MZ, Tahrani A, Herrmann F, Kaufmann D, Farrag N, El-Shazly A, Wink M: Chemical composition and biological activity of Citrus jambhiri Lush. Food Chem. 2011, 127: 394-403. 10.1016/j.foodchem.2010.12.129.CrossRefPubMed
16.
Nibret E, Ashour ML, Rubanza CD, Wink M: Screening of some Tanzanian medicinal plants for their trypanocidal and cytotoxic activities. Phytother Res. 2010, 24: 945-947.PubMed
17.
Huber W, Koella JC: A comparison of three methods of estimating EC50 in studies of drug resistance of malaria parasites. Acta Trop. 1993, 55: 257-261. 10.1016/0001-706X(93)90083-N.CrossRefPubMed
18.
Nibret E, Sporer F, Asres K, Wink M: Antitrypanosomal and cytotoxic activities of pyrrolizidine alkaloid-producing plants of Ethiopia. J Pharm Pharmacol. 2009, 61: 801-808. 10.1211/jpp.61.06.0014.CrossRefPubMed
19.
Mulyaningsih S, Sporer F, Reichling J, Wink M: Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharm Biol. 2011, 49: 893-899. 10.3109/13880209.2011.553625.CrossRefPubMed
20.
Abou-Karam M, Shier WT: A simplified plaque reduction assay for antiviral agents from plants. Demonstration of frequent occurrence of antiviral activity in higher plants. J Nat Prod. 1990, 53: 340-344. 10.1021/np50068a011.CrossRefPubMed
21.
Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983, 65: 55-63. 10.1016/0022-1759(83)90303-4.CrossRefPubMed
22.
Reimer TA, Anagnostopoulos I, Erdmann B, Lehmann I, Stein H, Daniel P, Dorken B, Rehm A: Reevaluation of the 22-1-1 antibody and its putative antigen, EBAG9/RCAS1, as a tumor marker. BMC Cancer. 2005, 5: 47-10.1186/1471-2407-5-47.PubMedCentralCrossRefPubMed
23.
Tasdemir D, Kaiser M, Brun R, Yardley V, Schmidt TJ, Tosun F, Ruedi P: Antitrypanosomal and antileishmanial activities of flavonoids and their analogues: in vitro, in vivo, structure-activity relationship, and quantitative structure-activity relationship studies. Antimicrob Agents Chemother. 2006, 50: 1352-1364. 10.1128/AAC.50.4.1352-1364.2006.PubMedCentralCrossRefPubMed
24.
Wink M: Medicinal plants: source of anti-parasitic secondary metabolites. Molecules. 2012, 17: 12771-12791. 10.3390/molecules171112771.CrossRefPubMed
25.
Mamadalieva NZ, Herrmann F, El-Readi MZ, Tahrani A, Hamoud R, Egamberdieva DR, Azimova SS, Wink M: Flavonoids in Scutellaria immaculata and S. ramosissima (Lamiaceae) and their biological activity. J Pharm Pharmacol. 2011, 63: 1346-1357. 10.1111/j.2042-7158.2011.01336.x.CrossRefPubMed
26.
Hoet S, Opperdoes F, Brun R, Quetin-Leclercq J: Natural products active against African trypanosomes: a step towards new drugs. Nat Prod Rep. 2004, 21: 353-364. 10.1039/b311021b.CrossRefPubMed
27.
Gertsch J, Tobler RT, Brun R, Sticher O, Heilmann J: Antifungal, antiprotozoal, cytotoxic and piscicidal properties of Justicidin B and a new arylnaphthalide lignan from Phyllanthus piscatorum. Planta Med. 2003, 69: 420-424.CrossRefPubMed
28.
Wink M: Evolutionary advantage and molecular modes of action of multi-component mixtures used in phytomedicine. Curr Drug Metab. 2008, 9: 996-1009. 10.2174/138920008786927794.CrossRefPubMed
29.
Ushio Y, Abe H: Inactivation of measles virus and herpes simplex virus by saikosaponin d. Planta Med. 1992, 58: 171-173. 10.1055/s-2006-961422.CrossRefPubMed
30.
Chiang LC, Ng LT, Liu LT, Shieh DE, Lin CC: Cytotoxicity and anti-hepatitis B virus activities of saikosaponins from Bupleurum species. Planta Med. 2003, 69: 705-709.CrossRefPubMed
31.
Cheng PW, Chiang LC, Yen MH, Lin CC: Bupleurum kaoi inhibits Coxsackie B virus type 1 infection of CCFS-1 cells by induction of type I interferons expression. Food Chem Toxicol. 2007, 45: 24-31. 10.1016/j.fct.2006.06.007.CrossRefPubMed
32.
33.
Wink M, Van Wyk B-E: Mind-altering and poisonous plants of the world. 2008, Portland: Timber Press
34.
Bedows E, Hatfield GM: An investigation of the antiviral activity of Podophyllum peltatum. J Nat Prod. 1982, 45: 725-729. 10.1021/np50024a015.CrossRefPubMed
35.
MacRae WD, Hudson JB, Towers GH: The antiviral action of lignans. Planta Med. 1989, 55: 531-535. 10.1055/s-2006-962087.CrossRefPubMed
36.
Jassim SA, Naji MA: Novel antiviral agents: a medicinal plant perspective. Appl Microbiol. 2003, 95: 412-427. 10.1046/j.1365-2672.2003.02026.x.CrossRef
37.
Cushnie TP, Lamb AJ: Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005, 26: 343-356. 10.1016/j.ijantimicag.2005.09.002.CrossRefPubMed
38.
Naithani R, Huma LC, Holland LE, Shukla D, McCormick DL, Mehta RG, Moriarty RM: Antiviral activity of phytochemicals: a comprehensive review. Mini-Rev Med Chem. 2008, 8: 1106-1133. 10.2174/138955708785909943.CrossRefPubMed
39.
Kaul TN, Middleton E, Ogra PL: Antiviral effect of flavonoids on human viruses. J Med Virol. 1985, 15: 71-79. 10.1002/jmv.1890150110.CrossRefPubMed
40.
Ohnishi E, Bannai H: Quercetin potentiates TNF-induced antiviral activity. Antiviral Res. 1993, 22: 327-331. 10.1016/0166-3542(93)90041-G.CrossRefPubMed
41.
Kim HJ, Woo ER, Shin CG, Park H: A new flavonol glycoside gallate ester from Acer okamotoanum and its inhibitory activity against human immunodeficiency virus-1 (HIV-1) integrase. J Nat Prod. 1998, 61: 145-148. 10.1021/np970171q.CrossRefPubMed
42.
Hsu YL, Kuo PL, Weng TC, Yen MH, Chiang LC, Lin CC: The antiproliferative activity of saponin-enriched fraction from Bupleurum Kaoi is through Fas-dependent apoptotic pathway in human non-small cell lung cancer A549 cells. Biol Pharm Bull. 2004, 27: 1112-1115. 10.1248/bpb.27.1112.CrossRefPubMed
43.
Cheng YL, Lee SC, Lin SZ, Chang WL, Chen YL, Tsai NM, Liu YC, Tzao C, Yu DS, Harn HJ: Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. Cancer Lett. 2005, 222: 183-193. 10.1016/j.canlet.2004.10.015.CrossRefPubMed
44.
Fujioka T, Yoshida K, Shibao H, Nagao T, Yoshida M, Matsunaga K, Takata J, Karube Y, Iwase Y, Okabe H, Mihashi K: Antiproliferative constituents from umbelliferae plants. IX. New triterpenoid glycosides from the fruits of Bupleurum rotundifolium. Chem Pharm Bull. 2006, 54: 1694-1704. 10.1248/cpb.54.1694.CrossRefPubMed
45.
Chen YL, Lin SZ, Chang WL, Cheng YL, Harn HJ: Requirement for ERK activation in acetone extract identified from Bupleurum scorzonerifolium induced A549 tumor cell apoptosis and keratin 8 phosphorylation. Life Sci. 2005, 76: 2409-2420. 10.1016/j.lfs.2004.09.044.CrossRefPubMed
46.
Csokay B, Prajda N, Weber G, Olah E: Molecular mechanisms in the antiproliferative action of quercetin. Life Sci. 1997, 60: 2157-2163. 10.1016/S0024-3205(97)00230-0.CrossRefPubMed
47.
Xavier CP, Lima CF, Preto A, Seruca R, Fernandes-Ferreira M, Pereira-Wilson C: Luteolin, quercetin and ursolic acid are potent inhibitors of proliferation and inducers of apoptosis in both KRAS and BRAF mutated human colorectal cancer cells. Cancer Lett. 2009, 281: 162-170. 10.1016/j.canlet.2009.02.041.CrossRefPubMed
48.
MacRae WD, Towers GHN: Biological activities of lignans. Phytochemistry. 1984, 23: 1207-1220. 10.1016/S0031-9422(00)80428-8.CrossRef
49.
Wink M: Plant secondary metabolism: diversity, function and its evolution. Nat Prod Commun. 2008, 3: 1205-1216.
50.
Seithel A, Karlsson J, Hilgendorf C, Bjorquist A, Ungell AL: Variability in mRNA expression of ABC- and SLC-transporters in human intestinal cells: comparison between human segments and Caco-2 cells. Eur J Pharm Sci. 2006, 28: 291-299. 10.1016/j.ejps.2006.03.003.CrossRefPubMed
51.
Wink M, Ashour ML, El-Readi MZ: Secondary metabolites from plants inhibiting ABC transporters and reversing resistance of cancer cells and microbes to cytotoxic and antimicrobial agents. Front Microbiol. 2012, 3: 130-PubMedCentralCrossRefPubMed
52.
El-Readi MZ, Hamdan D, Farrag N, El-Shazly A, Wink M: Inhibition of P-glycoprotein activity by limonin and other secondary metabolites from Citrus species in human colon and leukaemia cell lines. Eur J Pharmacol. 2010, 626: 139-145. 10.1016/j.ejphar.2009.09.040.CrossRefPubMed
Metadata
Title
Anti-infective and cytotoxic properties of Bupleurum marginatum
Authors
Mohamed L Ashour
Mahmoud Z El-Readi
Razan Hamoud
Safaa Y Eid
Sherweit H El Ahmady
Endalkachew Nibret
Florian Herrmann
Mahmoud Youns
Ahmed Tahrani
Dorothea Kaufmann
Michael Wink
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2014
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/1749-8546-9-4

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