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Chinese Medicine

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Open Access 01-12-2007 | Research

Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography

Authors: Jingzheng Song, Quanbin Han, Chunfeng Qiao, Yuekeung Yip, Hongxi Xu

Published in: Chinese Medicine | Issue 1/2007

Abstract

Background

Concentrated Gegen Tang (GT) granule is a widely available traditional Chinese medicinal product for the treatment of cold and flu. There was no reliable analytical method available for the quality assessment of GT granules.

Methods

An HPLC method with an Agilent Zorbax SB-Phenyl Stablebond column (250 × 4.6 mm, 5 μm) was developed and validated. The mobile phase gradient was a mixture of 0.1% trifluoroacetic acid (TFA) in acetonitrile (ACN) and 0.1% TFA in water. The detection with a diode-array detector was set at 207, 230, 250 and 275 nm. Seven components, namely puerarin, daidzein, paeoniflorin, cinnamic acid, glycyrrhizin, ephedrine and pseudoephedrine were selected as marker compounds for the evaluation.

Results

The regression equations revealed good linear relationships (correlation coefficients: 0.9994–0.9998) between the peak areas and concentrations. The recovery was between 98.8% and 101.7% with good precision and accuracy. The quality of GT granule from four different manufacturers was evaluated with this newly developed method. Samples from four manufacturers showed similar profiles but contents of the detected markers varied significantly among manufacturers and batches.

Conclusion

A new method using high performance liquid chromatography (HPLC) has been developed for simple and reliable quality control of commercial concentrated GT granules. Sensitivity was increased by multi-wavelength detection. The contents of selected marker components in GT granules varied significantly among manufacturers and batches, making it necessary to evaluate the quality of concentrated GT granules in the market.

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Title: Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography
Authors: Jingzheng Song
Quanbin Han
Chunfeng Qiao
Yuekeung Yip
Hongxi Xu
Publication date: 01-12-2007
Publisher: BioMed Central
Published in: Chinese Medicine / Issue 1/2007
Electronic ISSN: 1749-8546
DOI: https://doi.org/10.1186/1749-8546-2-7

At a glance: The STEP trials

Springer Medicine

Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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