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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2006

Open Access 01-12-2006 | Research article

Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs

Authors: Aaron M Stoker, James L Cook, Keiichi Kuroki, Derek B Fox

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2006

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Abstract

Background

Osteoarthritis (OA) is a progressive and debilitating disease that often develops from a focal lesion and may take years to clinically manifest to a complete loss of joint structure and function. Currently, there is not a cure for OA, but early diagnosis and initiation of treatment may dramatically improve the prognosis and quality of life for affected individuals. This study was designed to determine the feasibility of analyzing changes in gene expression of articular cartilage using the Pond-Nuki model two weeks after ACL-transection in dogs, and to characterize the changes observed at this time point.

Methods

The ACL of four dogs was completely transected arthroscopically, and the contralateral limb was used as the non-operated control. After two weeks the dogs were euthanatized and tissues harvested from the tibial plateau and femoral condyles of both limbs. Two dogs were used for histologic analysis and Mankin scoring. From the other two dogs the surface of the femoral condyle and tibial plateau were divided into four regions each, and tissues were harvested from each region for biochemical (GAG and HP) and gene expression analysis. Significant changes in gene expression were determined using REST-XL, and Mann-Whitney rank sum test was used to analyze biochemical data. Significance was set at (p < 0.05).

Results

Significant differences were not observed between ACL-X and control limbs for Mankin scores or GAG and HP tissue content. Further, damage to the tissue was not observed grossly by India ink staining. However, significant changes in gene expression were observed between ACL-X and control tissues from each region analyzed, and indicate that a unique regional gene expression profile for impending ACL-X induced joint pathology may be identified in future studies.

Conclusion

The data obtained from this study lend credence to the research approach and model for the characterization of OA, and the identification and validation of future diagnostic modalities. Further, the changes observed in this study may reflect the earliest changes in AC reported during the development of OA, and may signify pathologic changes within a stage of disease that is potentially reversible.
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Metadata
Title
Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs
Authors
Aaron M Stoker
James L Cook
Keiichi Kuroki
Derek B Fox
Publication date
01-12-2006
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2006
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/1749-799X-1-8

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