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Diagnostic Pathology
Published in: Diagnostic Pathology 1/2013

Open Access 01-12-2013 | Research

Upregulation of SOX9 in osteosarcoma and its association with tumor progression and patients’ prognosis

Authors: Haibo Zhu, Jie Tang, Mingjie Tang, Haikang Cai

Published in: Diagnostic Pathology | Issue 1/2013

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Abstract

Objective

SOX9 plays an important role in bone formation and tumorigenesis. However, its involvement in osteosarcoma is still unclear. The aim of this study was to investigate the expression pattern and the clinical significance of SOX9 in human osteosarcoma.

Methods

SOX9 mRNA and protein expression levels were detected by RT-PCR and Western blot assays, respectively, using 30 pairs of osteosarcoma and noncancerous bone tissues. Then, immunohistochemistry was performed to analyze the association of SOX9 expression in 166 osteosarcoma tissues with clinicopathological factors or survival of patients.

Results

SOX9 expression at mRNA and protein levels were both significantly higher in osteosarcoma tissues than those in corresponding noncancerous bone tissues (both P < 0.001). Immunohistochemical staining indicated that SOX9 localized to the nucleus and high SOX9 expression was observed in 120 of 166 (72.3%) osteosarcoma specimens. In addition, high SOX9 expression was more frequently occurred in osteosarcoma tissues with advanced clinical stage (P = 0.02), positive distant metastasis (P = 0.008) and poor response to chemotherapy (P = 0.02). Osteosarcoma patients with high SOX9 expression had shorter overall survival and disease-free survival (both P < 0.001). Furthermore, the multivariate analysis confirmed that upregulation of SOX9 was an independent and significant prognostic factor to predict poor overall survival and disease-free survival (both P = 0.006).

Conclusions

Our data show for the first time that SOX9 is upregulated in aggressive osteosarcoma tissues indicating that SOX9 may participate in the osteosarcoma progression. More importantly, SOX9 status is a useful prognostic factor for predicting the prognosis of osteosarcoma, suggesting that SOX9 may contribute to the optimization of clinical treatments for osteosarcoma patients.

Virtual slides

The virtual slides for this article can be found here: http://​www.​diagnosticpathol​ogy.​diagnomx.​eu/​vs/​1318085636110837​.
Appendix
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Literature
1.
Gill J, Ahluwalia MK, Geller D, Gorlick R: New targets and approaches in osteosarcoma. Pharmacol Ther. 2013, 137: 89-99.CrossRefPubMed
2.
Bielack SS, Kempf-Bielack B, Delling G, Exner GU, Flege S, Helmke K, Kotz R, Salzer-Kuntschik M, Werner M, Winkelmann W, Zoubek A, Jürgens H, Winkler K: Prognostic factors in high-grade osteosarcoma of the extremities or trunk: an analysis of 1,702 patients treated on neoadjuvant cooperative osteosarcoma study group protocols. J Clin Oncol. 2002, 20: 776-790.CrossRefPubMed
3.
Jaffe N: Adjuvant chemotherapy in osteosarcoma: An odyssey of rejection and vindication. Pediatric and Adolescent Osteosarcoma, Cancer Treatment and Research. Edited by: Jaffe N, Bielack SS, Bruland OS. 2009, New York: Springer, 152-
4.
Bakhshi S, Radhakrishnan V: Prognostic markers in osteosarcoma. Expert Rev Anticancer Ther. 2010, 10: 271-287.CrossRefPubMed
5.
Magnan H, Chou AJ, Chou JF, Yeung HW, Healey JH, Meyers PA: Noninvasive imaging with thallium-201 scintigraphy may not correlate with survival in patients with osteosarcoma. Cancer. 2010, 116: 4147-4151.CrossRefPubMed
6.
Jakob S, Lovell-Badge R: Sex determination and the control of Sox9 expression in mammals. FEBS J. 2011, 278: 1002-1009.CrossRefPubMed
7.
Barrionuevo F, Scherer G: SOX E genes: SOX9 and SOX8 in mammalian testis development. Int J Biochem Cell Biol. 2010, 42: 433-436.CrossRefPubMed
8.
Gordon CT, Tan TY, Benko S, Fitzpatrick D, Lyonnet S, Farlie PG: Long-range regulation at the SOX9 locus in development and disease. J Med Genet. 2009, 46: 649-656.CrossRefPubMed
9.
Zhou CJ, Guo JQ, Zhu KX, Zhang QH, Pan CR, Xu WH, Wang HJ, Liu B: Elevated expression of SOX9 is related with the progression of gastric carcinoma. Diagn Cytopathol. 2011, 39: 105-109.CrossRefPubMed
10.
Chakravarty G, Moroz K, Makridakis NM, Lloyd SA, Galvez SE, Canavello PR, Lacey MR, Agrawal K, Mondal D: Prognostic significance of cytoplasmic SOX9 in invasive ductal carcinoma and metastatic breast cancer. Exp Biol Med (Maywood). 2011, 236: 145-155.CrossRef
11.
Vidal VP, Ortonne N, Schedl A: SOX9 expression is a general marker of basal cell carcinoma and adnexal-related neoplasms. J Cutan Pathol. 2008, 35: 373-379.CrossRefPubMed
12.
Lü B, Fang Y, Xu J, Wang L, Xu F, Xu E, Huang Q, Lai M: Analysis of SOX9 expression in colorectal cancer. Am J Clin Pathol. 2008, 130: 897-904.CrossRefPubMed
13.
Huang Z, Hurley PJ, Simons BW, Marchionni L, Berman DM, Ross AE, Schaeffer EM: Sox9 is required for prostate development and prostate cancer initiation. Oncotarget. 2012, 3: 651-663.PubMedCentralCrossRefPubMed
14.
Zhou CH, Ye LP, Ye SX, Li Y, Zhang XY, Xu XY, Gong LY: Clinical significance of SOX9 in human non-small cell lung cancer progression and overall patient survival. J Exp Clin Cancer Res. 2012, 31: 18-PubMedCentralCrossRefPubMed
15.
Tanaka T, Kuroki T, Adachi T, Ono S, Hirabaru M, Soyama A, Kitasato A, Takatsuki M, Hayashi T, Eguchi S: Evaluation of SOX9 expression in pancreatic ductal adenocarcinoma and intraductal papillary mucinous neoplasm. Pancreas. 2013, 42: 488-493.CrossRefPubMed
16.
Wang L, He S, Yuan J, Mao X, Cao Y, Zong J, Tu Y, Zhang Y: Oncogenic role of SOX9 expression in human malignant glioma. Med Oncol. 2012, 29: 3484-3490.CrossRefPubMed
17.
Passeron T, Valencia JC, Namiki T, Vieira WD, Passeron H, Miyamura Y, Hearing VJ: Upregulation of SOX9 inhibits the growth of human and mouse melanomas and restores their sensitivity to retinoic acid. J Clin Invest. 2009, 119: 954-963.PubMedCentralPubMed
18.
Tang J, Cai H, Lin L, Xie P, Zhong W, Tang M: Increased expression of CD24 is associated with tumor progression and prognosis in patients suffering osteosarcoma. Clin Transl Oncol. 2013, 15: 541-547.CrossRefPubMed
19.
Won KY, Park HR, Park YK: Prognostic implication of immunohistochemical Runx2 expression in osteosarcoma. Tumori. 2009, 95: 311-316.PubMed
20.
Sarkar A, Hochedlinger K: The sox family of transcription factors: versatile regulators of stem and progenitor cell fate. Cell Stem Cell. 2013, 12: 15-30.PubMedCentralCrossRefPubMed
21.
22.
Knower KC, Kelly S, Harley VR: Turning on the male–SRY, SOX9 and sex determination in mammals. Cytogenet Genome Res. 2003, 101: 185-198.CrossRefPubMed
23.
Castillo SD, Sanchez-Cespedes M: The SOX family of genes in cancer development: biological relevance and opportunities for therapy. Expert Opin Ther Targets. 2012, 16: 903-919.CrossRefPubMed
Metadata
Title
Upregulation of SOX9 in osteosarcoma and its association with tumor progression and patients’ prognosis
Authors
Haibo Zhu
Jie Tang
Mingjie Tang
Haikang Cai
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Diagnostic Pathology / Issue 1/2013
Electronic ISSN: 1746-1596
DOI
https://doi.org/10.1186/1746-1596-8-183

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