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Published in: Trials 1/2011

Open Access 01-12-2011 | Research

Efficacy of essential oil mouthwash with and without alcohol: a 3-Day plaque accumulation model

Authors: Enrico Marchetti, Stefano Mummolo, Jonathan Di Mattia, Fabio Casalena, Salvatore Di Martino, Antonella Mattei, Giuseppe Marzo

Published in: Trials | Issue 1/2011

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Abstract

Background

The aim of this study was to evaluate the antiplaque effect of a new alcohol free essential oil mouthwash with respect to a control of an essential oil with alcohol mouthwash, using an in vivo plaque regrowth model of 3-days.

Methods

The study was designed as a double-masked, randomized, crossover clinical trial, involving 30 volunteers to compare two different essential oil containing mouthwashes, during a 3-day plaque accumulation model. After receiving a thorough professional prophylaxis at the baseline, over the next 3-days each volunteer refrained from all oral hygiene measures and had two daily rinses with 20 ml of the test mouthwash (alcohol free essential oil) or the control mouthwash (essential oil with alcohol). At the end of the each experimental period, plaque was assessed and the panelists filled out a questionnaire. Each subject underwent a 14 days washout period and there was a second allocation.

Results

The essential oil mouthwash with ethanol shows a better inhibitory effect of plaque regrowth in 3-days than the mouthwash test with only essential oil in the whole mouth (plaque index = 2.18 against 2.46, respectively, p < 0.05); for the lower jaw (plaque index = 2.28 against 2.57, respectively, p < 0.05); for the upper jaw (plaque index = 2.08 against 2.35, respectively, p < 0.05); for the incisors (plaque index = 1.93 against 2.27, respectively, p < 0.05); and the canines (plaque index = 1.99 against 2.47, respectively, p < 0.05).

Conclusion

The essential oil containing mouthwash without alcohol seems to have a less inhibiting effect on the plaque regrowth than the traditional alcoholic solution.

Trial Registration

ClinicalTrials.gov NCT01411618
Appendix
Available only for authorised users
Literature
1.
Beals D, Ngo T, Feng Y, Cook D, Grau DG, Weber DA: Development and laboratory evaluation of a new toothbrush with a novel brush head design. Am J Dent. 2000, 13: 5A-14A.PubMed
2.
Bader HI: Floss or die: implications for dental professionals. Dent Today. 1998, 17: 76-78. 80-82PubMed
3.
Stewart JE, Wolfe GR: The retention of newly-acquired brushing and flossing skills. J Clin Periodontol. 1989, 16: 331-332. 10.1111/j.1600-051X.1989.tb01664.x.CrossRefPubMed
4.
Lang NP, Hotz P, Graf H, Geering AH, Saxer UP, Sturzenberger OP, Mecker AH: Effects of supervised chlorhexidine mouthrinses in children. A longitudinal clinical trial. J Periodontal Res. 1982, 17: 101-111. 10.1111/j.1600-0765.1982.tb01135.x.CrossRefPubMed
5.
DePaola LG, Overholser CD, Meiller TF, Minah GE, Niehaus C: Chemotherapeutic inhibition of supragingival dental plaque and gingivitis development. J Clin Periodontol. 1989, 16: 311-315. 10.1111/j.1600-051X.1989.tb01661.x.CrossRefPubMed
6.
Fine DH, Markowitz K, Furgang D, Goldsmith D, Charles CH, Lisante TA, Lynch MC: Effect of an essential oil-containing antimicrobial mouthrinse on specific plaque bacteria in vivo. J Clin Periodontol. 2007, 34: 652-657. 10.1111/j.1600-051X.2007.01096.x.CrossRefPubMed
7.
Paraskevas S: Randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hyg. 2005, 3: 162-178. 10.1111/j.1601-5037.2005.00145.x.CrossRefPubMed
8.
Charles CH, Sharma NC, Galustians HJ, Qaqish J, McGuire JA, Vincent JW: Comparative efficacy of an antiseptic mouthrinse and an antiplaque/antigingivitis dentifrice. A six-month clinical trial. J Am Dent Assoc. 2001, 132: 670-675.CrossRefPubMed
9.
Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C: Comparative effects of 2 chemotherapeu tic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol. 1990, 17: 575-579. 10.1111/j.1600-051X.1990.tb01108.x.CrossRefPubMed
10.
Sharma NC, Charles CH, Qaqish JG, Galustians HJ, Zhao Q, Kumar LD: Comparative effectiveness of an essential oil mouthrinse and dental floss in controlling interproximal gingivitis and plaque. Am J Dent. 2002, 15: 351-355.PubMed
11.
Sharma N, Charles CH, Lynch MC, Qaqish JG, McGuire JA, Galustians HJ, Kumar LD: Adjunctive benefit of an essential oil-containing mouthrinse in reducing plaque and gingivitis in patients who brush and floss regularly. A six-month study. J Am Dent Assoc. 2004, 135: 496-504.CrossRefPubMed
12.
Charles CH, Mostler KM, Bartels LL, Mankodi SM: Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6-month clinical trial. J Clin Periodontol. 2004, 31: 878-884. 10.1111/j.1600-051X.2004.00578.x.CrossRefPubMed
13.
Pizzo G, La Cara M, Licata ME, Pizzo I, D'Angelo M: The effects of an essential oil and an amine fluoride/stannous fluoride mouthrinse on supragingival plaque regrowth. J Periodontology. 2008, 79: 1177-1183. 10.1902/jop.2008.070583.CrossRef
14.
Cortelli SC, Cortelli JR, Holzhausen M, Franco GCN, Rebelo RZ, Sonagere AS, Queiroz CS, Costa FO: Essential oils in one stage full-mouth disinfection: double- blind, randomized clinical trial of long-term clinical, microbial and salivary effects. J Clin Periodontol. 2009, 36: 333-342. 10.1111/j.1600-051X.2009.01376.x.CrossRefPubMed
15.
Fine DH: Mouthrinses as adjuncts for plaque and gingivitis management. A status report for the American Journal of Dentistry. Am J Dent. 1998, 1: 259-263.
16.
Kubert D, Rubin M, Barnett ML, Vincent JW: Antiseptic mouthrinse induced microbial cell surface alterations. Am J Dent. 1993, 6: 277-279.PubMed
17.
Dewhirst FE: Structure activity relationships for inhibition of prostaglandin cyclooxygenase by phenolic compounds. Prostaglandins. 1980, 20: 209-222. 10.1016/S0090-6980(80)80040-2.CrossRefPubMed
18.
Azuma Y, Ozasa N, Ueda Y, Takagi N: Pharmacological studies on the anti- inflammatory action of phenolic compounds. J Dent Res. 1986, 65: 53-56. 10.1177/00220345860650010901.CrossRefPubMed
19.
Pan P, Barnett ML, Coelho J, Brogdon C, Finnegan MB: Determination of the in situ bactericidal activity of an essential oil mouthrinse using a vital stain method. J Clin Periodontol. 2000, 27: 256-261. 10.1034/j.1600-051x.2000.027004256.x.CrossRefPubMed
20.
Fine DH, Furgang D, Barnett ML: Comparative antimicrobial activities of antiseptic mouthrinses against isogenic planktonic and biofilm forms of actinobacillus actinomycetemcomitans. J Clin Periodontol. 2001, 28: 697-700. 10.1034/j.1600-051x.2001.028007697.x.CrossRefPubMed
21.
Ouhayoun JP: Penetrating the plaque biofilm: impact of essential oil mouthwash. J Clin Periodont. 2003, 30 (Suppl 5): 10-12.CrossRef
22.
Sissons CH, Wong L, Cutress TW: Inhibition by ethanol of the growth of biofilm and dispersed microcosm dental plaques. Arch Oral Biol. 1996, 41: 27-34. 10.1016/0003-9969(95)00103-4.CrossRefPubMed
23.
Marchetti E, Mummulo S, Marzo G, Civisca A, Di Pietro C, Marinelli G: Effect of an essential-oil containing mouthrinse on the reduction of plaque and interproximal inflammation in comparison with an ethanol solution. Prev Assist Dent. 2009, 35 (4): 123-127. 10.1016/j.pad.2009.04.003.CrossRef
24.
Penugonda B, Settembrini L, Scherer W, Hittelmann E, Strassler H: Alcohol-containing mouthwashes: effect on composite hardness. J Clin Dent. 1994, 5: 60-62.PubMed
25.
Smigel K: High alcohol mouthwashes are under scrutiny. J Natl Cancer Inst. 1991, 83: 751-10.1093/jnci/83.11.751.CrossRefPubMed
26.
27.
La Vecchia C: Mouthwash and oral cancer risk: An update. Oral Oncol. 2009, 45: 198-200. 10.1016/j.oraloncology.2008.08.012.CrossRefPubMed
28.
Turesky S, Gilmore ND, Glickman I: Reduced plaque formation by the chloromethyl analogue of vitamin C. J Periodontol. 1970, 41: 41-43.CrossRefPubMed
29.
Paraskevas S, Rosema NA, Versteeg P, Van der Velden U, Van der Weijden GA: Chlorine dioxide and chlorhexidine mouthrinses compared in a 3-day plaque accumulation model. J Periodontol. 2008, 79: 1395-1400. 10.1902/jop.2008.070630.CrossRefPubMed
30.
Brecx M, Netuschil L, Reichert B, Schreil G: Efficacy of Listerine, Meridol and chlorhexidine mouthrinses on plaque, gingivitis and bacteria vitality. J Clin Periodontol. 1990, 17: 292-297. 10.1111/j.1600-051X.1990.tb01092.x.CrossRefPubMed
31.
Brecx M, Brownstone E, MacDonald L, Gelskey S, Cheang M: Efficacy of Listerine, Meridol and chlorhexidine mouthrinses as supplements to regular tooth-cleaning measures. J Clin Periodontol. 1992, 19: 202-207. 10.1111/j.1600-051X.1992.tb00640.x.CrossRefPubMed
32.
Moran J, Addy M, Newcombe RG, Warren P: The comparative effects on plaque regrowth of phenolic chlorhexidine and anti-adhesive mouthrinses. J Clin Periodontol. 1995, 22: 929-934. 10.1111/j.1600-051X.1995.tb01797.x.CrossRefPubMed
33.
Moran J, Addy M, Kohut B, Hovliaras CA, Newcombe RG: Efficacy of mouthrinses in inhibiting the development of supragingival plaque over a 4-day period of no oral hygiene. J Periodontol. 1994, 65: 904-907.CrossRefPubMed
34.
Rosin M, Welk A, Kocher T, Majic-Todt A, Kramer A, Pitten FA: The effect of a polyhexamethylene biguanide mouthrinse compared to an essential oil rinse and a chlorhexidine rinse on bacterial counts and 4-day plaque regrowth. J Clin Periodontol. 2002, 29: 392-399. 10.1034/j.1600-051X.2002.290503.x.CrossRefPubMed
35.
Riep BG, Bernimoulin JP, Barnett ML: Comparative antiplaque effectiveness of an essential oil and an amine fluoride/stannous fluoride mouthrinse. J Clin Periodontol. 1999, 26: 164-168. 10.1034/j.1600-051X.1999.260306.x.CrossRefPubMed
36.
Gordon JM, Lamster IB, Geiger MC: Efficacy of Listerine antiseptic in inhibiting the development of plaque and gingivitis. J Clin Periodontol. 1985, 12: 697-704. 10.1111/j.1600-051X.1985.tb00941.x.CrossRefPubMed
37.
Barnett ML: The role of therapeutic antimicrobial mouthrinses in clinical practice. Control of supragingival plaque and gingivitis. J Am Dent Assoc. 2003, 134: 699-704.CrossRefPubMed
38.
Van Leeuwen MP, Slot DE, Van der Weijden GA: Essential oils compared to chlorhexidine with respect to plaque and parameters of gingival inflammation: a systematic review. J Periodontology. 2011, 82: 174-194. 10.1902/jop.2010.100266.CrossRef
39.
Squier CA, Cox P, Hall BK: Enhanced penetration of nitroso-nornicotine across oral mucosa in the presence of ethanol. J Oral Pathol. 1986, 15: 276-279. 10.1111/j.1600-0714.1986.tb00623.x.CrossRefPubMed
40.
Howie NM, Trigkas TK, Cruchley AT, Wertz PW, Squier CA, Williams DM: Short-term exposure to alcohol increases the permeability of human oral mucosa. Oral Dis. 2001, 7: 349-354. 10.1034/j.1601-0825.2001.00731.x.CrossRefPubMed
41.
WHO International Agency for Research on Cancer: Re-evaluation of some organic chemicals, hydrazine and hydrogen peroxide. IARC Monografh on the Evaluation of Carcinogenic Risk to Humans. 1999, Lyon: IARC, 71 (Part 1): 1-315.
42.
Elmore JG, Horwitz RI: Oral cancer and mouthwash use: Evaluation of the epidemiologic evidence. Otolaryngol Head Neck Surg. 1995, 113: 253-261. 10.1016/S0194-5998(95)70114-1.CrossRefPubMed
43.
Lemos-Júnior CA, Villoria GEM: Reviewed evidence about the safety of the daily use of alcohol-based mouthrinses. Braz Oral Res. 2008, 22 (Suppl): 24S-31S.CrossRef
44.
Werner CW, Seymour RA: Are alcohol containing mouthwashes safe?. Br Dent J. 2009, 28: 207-E19
45.
McCullough MJ, Farah CS: The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes. Aust Dent J. 2008, 53: 302-305. 10.1111/j.1834-7819.2008.00070.x.CrossRefPubMed
46.
Simonsson T: Aspects of dental plaque formation with special reference to colloid-chemical phenomena. Swed Dent J Suppl. 1989, 58: 1-67.PubMed
47.
Zee KY, Samaranayake LP, Attstrom R: Scanning electron microscopy of microbial colonization of 'rapid' and 'slow' dental-plaque formers in vivo. Arch Oral Biol. 1997, 42: 735-742. 10.1016/S0003-9969(97)00059-9.CrossRefPubMed
48.
Van der Weijden GA, Timmerman MF, Novotny AGA, Rosema NAM, Verkerk AAJ: Three different rinsing times and inhibition of plaque accumulation with chlorhexidine. J Clin Periodontol. 2005, 32: 89-92. 10.1111/j.1600-051X.2004.00640.x.CrossRefPubMed
49.
Van Strydonck DAC, Timmerman MF, van der Velden U, van der Weijden GA: Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol. 2005, 32: 305-309. 10.1111/j.1600-051X.2005.00681.x.CrossRefPubMed
Metadata
Title
Efficacy of essential oil mouthwash with and without alcohol: a 3-Day plaque accumulation model
Authors
Enrico Marchetti
Stefano Mummolo
Jonathan Di Mattia
Fabio Casalena
Salvatore Di Martino
Antonella Mattei
Giuseppe Marzo
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Trials / Issue 1/2011
Electronic ISSN: 1745-6215
DOI
https://doi.org/10.1186/1745-6215-12-262

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