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Molecular Pain
Published in: Molecular Pain 1/2008

Open Access 01-12-2008 | Research

Effect of excitatory and inhibitory agents and a glial inhibitor on optically-recorded primary-afferent excitation

Authors: Hiroshi Ikeda, Takaki Kiritoshi, Kazuyuki Murase

Published in: Molecular Pain | Issue 1/2008

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Abstract

The effects of GABA, excitatory amino-acid receptors antagonists and a glial metabolism inhibitor on primary-afferent excitation in the spinal dorsal horn were studied by imaging the presynaptic excitation of high-threshold afferents in cord slices from young rats with a voltage-sensitive dye. Primary afferent fibers and terminals were anterogradely labeled with a voltage-sensitive dye from the dorsal root attached to the spinal cord slice. Single-pulse stimulation of C fiber-activating strength to the dorsal root elicited compound action potential-like optical responses in the superficial dorsal horn. The evoked presynaptic excitation was increased by the GABAA receptor antagonists picrotoxin and bicuculline, by glutamate receptor antagonists D-AP5 and CNQX, and by the glial metabolism inhibitor mono-fluoroacetic acid (MFA). The increase in presynaptic excitation by picrotoxin was inhibited in the presence of D-AP5, CNQX and MFA. Presynaptic modulation in the central terminal of fine primary afferents by excitatory and inhibitory amino acids may represent a mechanism that regulates the transmission of pain.
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Metadata
Title
Effect of excitatory and inhibitory agents and a glial inhibitor on optically-recorded primary-afferent excitation
Authors
Hiroshi Ikeda
Takaki Kiritoshi
Kazuyuki Murase
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Molecular Pain / Issue 1/2008
Electronic ISSN: 1744-8069
DOI
https://doi.org/10.1186/1744-8069-4-39

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