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Published in: Fluids and Barriers of the CNS 1/2010

Open Access 01-12-2010 | Research

Microarray-based gene expression profiling and DNA copy number variation analysis of temporal fossa arachnoid cysts

Authors: Mads Aarhus, Christian A Helland, Morten Lund-Johansen, Knut Wester, Per M Knappskog

Published in: Fluids and Barriers of the CNS | Issue 1/2010

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Abstract

Background

Intracranial arachnoid cysts (AC) are membranous sacs filled with CSF-like fluid that are commonly found in the temporal fossa. The majority of ACs are congenital. Typical symptoms are headache, dizziness, and dyscognition. Little is known about genes that contribute to the formation of the cyst membranes.

Methods

In order to identify differences in gene expression between normal arachnoid membrane (AM) and cyst membrane, we have performed a high-resolution mRNA microarray analysis. In addition we have screened DNA from AC samples for chromosomal duplications or deletions using DNA microarray-based copy number variation analysis.

Results

The transcriptome consisting of 33096 gene probes showed a near-complete similarity in expression between AC and AM samples. Only nine genes differed in expression between the two tissues: ASGR1, DPEP2, SOX9, SHROOM3, A2BP1, ATP10D, TRIML1, NMU were down regulated, whereas BEND5 was up regulated in the AC samples. Three of the AC samples had unreported human DNA copy number variations, all DNA gains.

Conclusions

Extending results of previous anatomical studies, the present study has identified a small subset of differentially expressed genes and DNA alterations in arachnoid cysts compared to normal arachnoid membrane.
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Metadata
Title
Microarray-based gene expression profiling and DNA copy number variation analysis of temporal fossa arachnoid cysts
Authors
Mads Aarhus
Christian A Helland
Morten Lund-Johansen
Knut Wester
Per M Knappskog
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Fluids and Barriers of the CNS / Issue 1/2010
Electronic ISSN: 2045-8118
DOI
https://doi.org/10.1186/1743-8454-7-6

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