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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2010

Open Access 01-12-2010 | Research

Membrane attack complex inhibitor CD59a protects against focal cerebral ischemia in mice

Authors: Denise Harhausen, Uldus Khojasteh, Philip F Stahel, B Paul Morgan, Wilfried Nietfeld, Ulrich Dirnagl, George Trendelenburg

Published in: Journal of Neuroinflammation | Issue 1/2010

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Abstract

Background

The complement system is a crucial mediator of inflammation and cell lysis after cerebral ischemia. However, there is little information about the exact contribution of the membrane attack complex (MAC) and its inhibitor-protein CD59.

Methods

Transient focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in young male and female CD59a knockout and wild-type mice. Two models of MCAO were applied: 60 min MCAO and 48 h reperfusion, as well as 30 min MCAO and 72 h reperfusion. CD59a knockout animals were compared to wild-type animals in terms of infarct size, edema, neurological deficit, and cell death.

Results and Discussion

CD59a-deficiency in male mice caused significantly increased infarct volumes and brain swelling when compared to wild-type mice at 72 h after 30 min-occlusion time, whereas no significant difference was observed after 1 h-MCAO. Moreover, CD59a-deficient mice had impaired neurological function when compared to wild-type mice after 30 min MCAO.

Conclusion

We conclude that CD59a protects against ischemic brain damage, but depending on the gender and the stroke model used.
Appendix
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Metadata
Title
Membrane attack complex inhibitor CD59a protects against focal cerebral ischemia in mice
Authors
Denise Harhausen
Uldus Khojasteh
Philip F Stahel
B Paul Morgan
Wilfried Nietfeld
Ulrich Dirnagl
George Trendelenburg
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2010
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/1742-2094-7-15

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