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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2009

Open Access 01-12-2009 | Research

Immunomodulatory responses of peripheral blood mononuclear cells from multiple sclerosis patients upon in vitro incubation with the flavonoid luteolin: additive effects of IFN-β

Authors: Zohara Sternberg, Kailash Chadha, Alicia Lieberman, Allison Drake, David Hojnacki, Bianca Weinstock-Guttman, Frederick Munschauer

Published in: Journal of Neuroinflammation | Issue 1/2009

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Abstract

The study is aimed to determine the role of luteolin (3',4',5,7-tetrahydroxyflavone), alone and in combination with human interferon-beta (IFN-β), in modulating the immune response(s) of peripheral blood mononuclear cells (PBMCs) isolated from multiple sclerosis (MS) patients. PBMC proliferation in the presence or absence of these drugs was determined and the production of pro-inflammatory cytokines (IL-1β, TNF-α), and the ratio of cell migration mediator MMP-9, and its inhibitor, TIMP-1 was assessed in the culture supernatants. Luteolin reduced, in a dose-dependent manner, the proliferation of PBMCs, and modulated the levels of IL-1β and TNF-α released by PBMCs in the culture supernatants. Luteolin reduced the MMP-9/TIMP-1 ratio via lowering MMP-9 production. In the majority of cases, luteolin, when combined with IFN-β, had additive effects in modulating cell proliferation, IL-1β, TNF-α, MMP-9 and TIMP-1.
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Metadata
Title
Immunomodulatory responses of peripheral blood mononuclear cells from multiple sclerosis patients upon in vitro incubation with the flavonoid luteolin: additive effects of IFN-β
Authors
Zohara Sternberg
Kailash Chadha
Alicia Lieberman
Allison Drake
David Hojnacki
Bianca Weinstock-Guttman
Frederick Munschauer
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2009
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/1742-2094-6-28

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