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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2008

Open Access 01-12-2008 | Research

Hematopoietic cell activation in the subventricular zone after Theiler's virus infection

Authors: Gwendolyn E Goings, Adriana Greisman, Rachel E James, Leanne KF Abram, Wendy Smith Begolka, Stephen D Miller, Francis G Szele

Published in: Journal of Neuroinflammation | Issue 1/2008

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Abstract

Background

The periventricular subventricular zone (SVZ) contains stem cells and is an area of active neurogenesis and migration. Since inflammation can reduce neurogenesis, we tested whether Theiler's murine encephalomyelitis virus (TMEV) induces inflammation and reduces neurogenesis in the SVZ.

Methods

We performed immmunohistochemistry for the hematopoietic cell marker CD45 throughout the central nervous system and then examined neuroblasts in the SVZ.

Results

CD45+ activation (inflammation) occurred early in the forebrain and preceded cerebellar and spinal cord inflammation. Inflammation in the brain was regionally stochastic except for the SVZ and surrounding periventricular regions where it was remarkably pronounced and consistent. In preclinical mice, SVZ neuroblasts emigrated into inflamed periventricular regions. The number of proliferating phoshpohistone3+ cells and Doublecortin+ (Dcx) SVZ neuroblasts was overall unaffected during the periods of greatest inflammation. However the number of Dcx+ and polysialylated neural cell adhesion molecule (PSA-NCAM+) SVZ neuroblasts decreased only after periventricular inflammation abated.

Conclusion

Our results suggest that after TMEV infection, the SVZ may mount an attempt at neuronal repair via emigration, a process dampened by decreases in neuroblast numbers.
Appendix
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Metadata
Title
Hematopoietic cell activation in the subventricular zone after Theiler's virus infection
Authors
Gwendolyn E Goings
Adriana Greisman
Rachel E James
Leanne KF Abram
Wendy Smith Begolka
Stephen D Miller
Francis G Szele
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2008
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/1742-2094-5-44

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