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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2007

Open Access 01-12-2007 | Research

Microglia activation in sepsis: a case-control study

Authors: Afina W Lemstra, Jacqueline CM Groen in't Woud, Jeroen JM Hoozemans, Elise S van Haastert, Annemiek JM Rozemuller, Piet Eikelenboom, Willem A van Gool

Published in: Journal of Neuroinflammation | Issue 1/2007

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Abstract

Background

infection induces an acute phase response that is accompanied by non-specific symptoms collectively named sickness behavior. Recent observations suggest that microglial cells play a role in mediating behavioral changes in systemic infections. In animal models for sepsis it has been shown that after inducing lipopolysaccharide, LPS, microglia in the brain were activated. The aim of this study was to investigate whether activation of microglia can be detected in patients who died of sepsis.

Methods

in a case-control study brain tissue of 13 patients who died with sepsis was compared with that of 17 controls. Activated microglia were identified by expression of MHC-class II antigens and CD68. Microglia activation was analyzed by a semiquantitative score combining both the number of the immunoreactive cells and their morphology.

Results

in patients who died with sepsis there was a significant increase in activated microglia in the grey matter when stained with CD68 compared to controls. This effect was independent of the effect of age.

Conclusion

this study shows for the first time in human brain tissue an association between a systemic infection and activation of microglia in the brain. Activated microglia during sepsis could play a role in behavioral changes associated with systemic infection.
Appendix
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Metadata
Title
Microglia activation in sepsis: a case-control study
Authors
Afina W Lemstra
Jacqueline CM Groen in't Woud
Jeroen JM Hoozemans
Elise S van Haastert
Annemiek JM Rozemuller
Piet Eikelenboom
Willem A van Gool
Publication date
01-12-2007
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2007
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/1742-2094-4-4

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