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Published in: Journal of the International Society of Sports Nutrition 1/2012

Open Access 01-12-2012 | Research article

β-alanine supplementation improves isometric endurance of the knee extensor muscles

Authors: Craig Sale, Chester A Hill, James Ponte, Roger C Harris

Published in: Journal of the International Society of Sports Nutrition | Issue 1/2012

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Abstract

Background

We examined the effect of four weeks of β-alanine supplementation on isometric endurance of the knee extensors at 45% maximal voluntary isometric contraction (MVIC).

Methods

Thirteen males (age 23 ± 6 y; height 1.80 ± 0.05 m; body mass 81.0 ± 10.5 kg), matched for pre-supplementation isometric endurance, were allocated to either a placebo (n = 6) or β-alanine (n = 7; 6.4 g·d-1 over 4 weeks) supplementation group. Participants completed an isometric knee extension test (IKET) to fatigue, at an intensity of 45% MVIC, before and after supplementation. In addition, two habituation tests were completed in the week prior to the pre-supplementation test and a further practice test was completed in the week prior to the post-supplementation test. MVIC force, IKET hold-time, and impulse generated were recorded.

Results

IKET hold-time increased by 9.7 ± 9.4 s (13.2%) and impulse by 3.7 ± 1.3 kN·s-1 (13.9%) following β-alanine supplementation. These changes were significantly greater than those in the placebo group (IKET: t(11) = 2.9, p ≤0.05; impulse: t(11) = 3.1, p ≤ 0.05). There were no significant changes in MVIC force in either group.

Conclusion

Four weeks of β-alanine supplementation at 6.4 g·d-1 improved endurance capacity of the knee extensors at 45% MVIC, which most likely results from improved pH regulation within the muscle cell as a result of elevated muscle carnosine levels.
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Literature
1.
go back to reference Hultman E, Sahlin K: Acid–base balance during exercise. Exerc Sport Sci Rev. 1980, 8: 41-128.PubMed Hultman E, Sahlin K: Acid–base balance during exercise. Exerc Sport Sci Rev. 1980, 8: 41-128.PubMed
2.
go back to reference Sahlin K, Harris RC, Nylind B, Hultman E: Lactate content and pH in muscle obtained after dynamic exercise. Pflugers Archives. 1976, 367: 143-149. 10.1007/BF00585150.CrossRef Sahlin K, Harris RC, Nylind B, Hultman E: Lactate content and pH in muscle obtained after dynamic exercise. Pflugers Archives. 1976, 367: 143-149. 10.1007/BF00585150.CrossRef
3.
go back to reference Pan JW, Hamm JR, Hetherington HP, Rothman DL, Shulman RG: Correlation of lactate and pH in human skeletal muscle after exercise by 1H NMR. Magn Reson Med Sci. 1991, 20: 57-65. 10.1002/mrm.1910200107.CrossRef Pan JW, Hamm JR, Hetherington HP, Rothman DL, Shulman RG: Correlation of lactate and pH in human skeletal muscle after exercise by 1H NMR. Magn Reson Med Sci. 1991, 20: 57-65. 10.1002/mrm.1910200107.CrossRef
4.
go back to reference Spriet LL, Lindinger MI, McKelvie RS, Heigenhauser GJF, Jones NL: Muscle glycogenolysis and H+ concentration during maximal intermittent cycling. J Appl Physiol. 1989, 66: 8-13.PubMed Spriet LL, Lindinger MI, McKelvie RS, Heigenhauser GJF, Jones NL: Muscle glycogenolysis and H+ concentration during maximal intermittent cycling. J Appl Physiol. 1989, 66: 8-13.PubMed
5.
go back to reference Harris RC, Edwards RHT, Hultman E, Nordesjo LO, Nylind B: The time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man. Pflugers Archives. 1976, 367: 137-142. 10.1007/BF00585149.CrossRef Harris RC, Edwards RHT, Hultman E, Nordesjo LO, Nylind B: The time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man. Pflugers Archives. 1976, 367: 137-142. 10.1007/BF00585149.CrossRef
6.
go back to reference Sahlin K, Harris RC: The creatine kinase reaction: a simple reaction with functional complexity. Amino Acids. 2011, 40: 1363-1367. 10.1007/s00726-011-0856-8.CrossRefPubMed Sahlin K, Harris RC: The creatine kinase reaction: a simple reaction with functional complexity. Amino Acids. 2011, 40: 1363-1367. 10.1007/s00726-011-0856-8.CrossRefPubMed
7.
go back to reference Wallimann T, Tokarska-Schlattner M, Schlattner U: The creatine kinase system and pleiotropic effects of creatine. Amino Acids. 2011, 40: 1271-1296. 10.1007/s00726-011-0877-3.PubMedCentralCrossRefPubMed Wallimann T, Tokarska-Schlattner M, Schlattner U: The creatine kinase system and pleiotropic effects of creatine. Amino Acids. 2011, 40: 1271-1296. 10.1007/s00726-011-0877-3.PubMedCentralCrossRefPubMed
8.
go back to reference Trivedi B, Daniforth WH: Effect of pH on the kinetics of frog muscle phosphofructokinase. J Biol Chem. 1966, 241: 4110-4112.PubMed Trivedi B, Daniforth WH: Effect of pH on the kinetics of frog muscle phosphofructokinase. J Biol Chem. 1966, 241: 4110-4112.PubMed
9.
go back to reference Donaldson SKB, Hermansen L: Differential direct effects of H + and Ca2+ − activated force of skinned fibres from the soleus, cardiac, adductor magnus muscle of rabbits. Pflugers Archive. 1978, 376: 55-65. 10.1007/BF00585248.CrossRef Donaldson SKB, Hermansen L: Differential direct effects of H + and Ca2+ − activated force of skinned fibres from the soleus, cardiac, adductor magnus muscle of rabbits. Pflugers Archive. 1978, 376: 55-65. 10.1007/BF00585248.CrossRef
10.
go back to reference Fabiato A, Fabiato F: Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiac and skeletal muscles. J Physiol. 1978, 276: 233-235.PubMedCentralCrossRefPubMed Fabiato A, Fabiato F: Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiac and skeletal muscles. J Physiol. 1978, 276: 233-235.PubMedCentralCrossRefPubMed
11.
go back to reference Mannion AF, Jakeman PM, Dunnett M, Harris RC, Willian PL: Carnosine and anserine concentrations in the quadriceptsfemoris muscle of healthy humans. Eur J Appl Physiol. 1992, 64: 47-50. 10.1007/BF00376439.CrossRef Mannion AF, Jakeman PM, Dunnett M, Harris RC, Willian PL: Carnosine and anserine concentrations in the quadriceptsfemoris muscle of healthy humans. Eur J Appl Physiol. 1992, 64: 47-50. 10.1007/BF00376439.CrossRef
12.
go back to reference Bate-Smith EC: The buffering of muscle in rigour: protein, phosphate and carnosine. J Physiol. 1938, 92: 336-343.CrossRef Bate-Smith EC: The buffering of muscle in rigour: protein, phosphate and carnosine. J Physiol. 1938, 92: 336-343.CrossRef
13.
go back to reference Harris RC, Marlin DJ, Dunnett M, Snow DH, Hultman E: Muscle buffering capacity and dipeptide content in the thoroughbred horse, greyhound dog and man. Comp Physiol Biochem. 1990, 97A: 249-251.CrossRef Harris RC, Marlin DJ, Dunnett M, Snow DH, Hultman E: Muscle buffering capacity and dipeptide content in the thoroughbred horse, greyhound dog and man. Comp Physiol Biochem. 1990, 97A: 249-251.CrossRef
14.
go back to reference Harris RC, Tallon MJ, Dunnett M, Boobis LH, Coakley J, Kim HJ, Fallowfield JL, Hill CA, Sale C, Wise JA: The absorption of orally supplied β-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino Acids. 2006, 30: 279-289. 10.1007/s00726-006-0299-9.CrossRefPubMed Harris RC, Tallon MJ, Dunnett M, Boobis LH, Coakley J, Kim HJ, Fallowfield JL, Hill CA, Sale C, Wise JA: The absorption of orally supplied β-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino Acids. 2006, 30: 279-289. 10.1007/s00726-006-0299-9.CrossRefPubMed
15.
go back to reference Hobson RM, Saunders B, Ball G, Harris RC, Sale C: Effects of beta-alanine supplementation on exercise performance: a review by meta-analysis. Amino Acids. 2012, 43: 25-37. 10.1007/s00726-011-1200-z.PubMedCentralCrossRefPubMed Hobson RM, Saunders B, Ball G, Harris RC, Sale C: Effects of beta-alanine supplementation on exercise performance: a review by meta-analysis. Amino Acids. 2012, 43: 25-37. 10.1007/s00726-011-1200-z.PubMedCentralCrossRefPubMed
16.
go back to reference Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH, Kim CK, Wise JA: Influence of β-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity. Amino Acids. 2007, 32: 225-233. 10.1007/s00726-006-0364-4.CrossRefPubMed Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH, Kim CK, Wise JA: Influence of β-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity. Amino Acids. 2007, 32: 225-233. 10.1007/s00726-006-0364-4.CrossRefPubMed
17.
go back to reference Sale C, Saunders B, Hudson S, Wise JA, Harris RC, Sunderland CD: Effect of beta-alanine plus sodium bicarbonate on high-intensity cycling capacity. Med Sci Sports Exerc. 2011, 43: 1972-1978.PubMed Sale C, Saunders B, Hudson S, Wise JA, Harris RC, Sunderland CD: Effect of beta-alanine plus sodium bicarbonate on high-intensity cycling capacity. Med Sci Sports Exerc. 2011, 43: 1972-1978.PubMed
18.
go back to reference Sale C, Saunders B, Harris RC: Effect of beta-alanine supplementation on muscle carnosine concentrations and exercise performance. Amino Acids. 2010, 39: 321-333. 10.1007/s00726-009-0443-4.CrossRefPubMed Sale C, Saunders B, Harris RC: Effect of beta-alanine supplementation on muscle carnosine concentrations and exercise performance. Amino Acids. 2010, 39: 321-333. 10.1007/s00726-009-0443-4.CrossRefPubMed
19.
go back to reference Bonde-Petersen F, Mork Al, Nielsen E: Local muscle blood flow and sustained contractions of human arm and back muscles. Eur J Appl Physiol. 1975, 34: 43-50. 10.1007/BF00999914.CrossRef Bonde-Petersen F, Mork Al, Nielsen E: Local muscle blood flow and sustained contractions of human arm and back muscles. Eur J Appl Physiol. 1975, 34: 43-50. 10.1007/BF00999914.CrossRef
20.
go back to reference Sjogaard G, Savard G, Juel C: Muscle blood flow during isometric activity and its relation to muscle fatigue. Eur J Appl Physiol. 1988, 57: 327-335. 10.1007/BF00635992.CrossRef Sjogaard G, Savard G, Juel C: Muscle blood flow during isometric activity and its relation to muscle fatigue. Eur J Appl Physiol. 1988, 57: 327-335. 10.1007/BF00635992.CrossRef
21.
go back to reference De Ruitter CJ, Goudsmit JFA, Van Tricht JA, De Haan A: The isometric torque at which knee-extensor muscle reoxygenation stops. Med Sci Sports Exerc. 2007, 39: 443-452. 10.1249/mss.0b013e31802dd3cc.CrossRef De Ruitter CJ, Goudsmit JFA, Van Tricht JA, De Haan A: The isometric torque at which knee-extensor muscle reoxygenation stops. Med Sci Sports Exerc. 2007, 39: 443-452. 10.1249/mss.0b013e31802dd3cc.CrossRef
22.
go back to reference Rohmert W: Determination of the recovery pause for static work of man. Int Z Angew Physiol. 1960, 18: 123-164.PubMed Rohmert W: Determination of the recovery pause for static work of man. Int Z Angew Physiol. 1960, 18: 123-164.PubMed
23.
go back to reference Harris RC: Muscle energy metabolism in man in response to isometric contraction: A biopsy study. 1981, M.Sc. thesis, University of Bangor, Wales Harris RC: Muscle energy metabolism in man in response to isometric contraction: A biopsy study. 1981, M.Sc. thesis, University of Bangor, Wales
24.
go back to reference Ahlborg B, Bergström J, Ekelund L, Guarnieri G, Harris RC, Hultman E, Nordesjö L: Muscle metabolism during isometric exercise performed at constant force. J Appl Physiol. 1972, 33: 224-228.PubMed Ahlborg B, Bergström J, Ekelund L, Guarnieri G, Harris RC, Hultman E, Nordesjö L: Muscle metabolism during isometric exercise performed at constant force. J Appl Physiol. 1972, 33: 224-228.PubMed
25.
go back to reference Baguet A, Reyngoudt H, Pottier A, Everaert I, Callens S, Achten E, Derave W: Carnosine loading and washout in human skeletal muscles. J Appl Physiol. 2009, 106: 837-842. 10.1152/japplphysiol.91357.2008.CrossRefPubMed Baguet A, Reyngoudt H, Pottier A, Everaert I, Callens S, Achten E, Derave W: Carnosine loading and washout in human skeletal muscles. J Appl Physiol. 2009, 106: 837-842. 10.1152/japplphysiol.91357.2008.CrossRefPubMed
26.
go back to reference Derave W, Ozdemir MS, Harris RC, Pottier A, Reyngoudt H, Koppo K, Wise JA, Achten E: β-alanine supplementation augments muscle carnosine content and attenuates fatigue during repeated isokinetic contraction bouts in trained sprinters. J Appl Physiol. 2007, 103: 1736-1743. 10.1152/japplphysiol.00397.2007.CrossRefPubMed Derave W, Ozdemir MS, Harris RC, Pottier A, Reyngoudt H, Koppo K, Wise JA, Achten E: β-alanine supplementation augments muscle carnosine content and attenuates fatigue during repeated isokinetic contraction bouts in trained sprinters. J Appl Physiol. 2007, 103: 1736-1743. 10.1152/japplphysiol.00397.2007.CrossRefPubMed
27.
go back to reference Dutka TL, Lamb GD: Effect of carnosine on excitation-contraction coupling in mechanically-skinned rat skeletal muscle. J Muscle Res Cell Motil. 2004, 25: 203-213.CrossRefPubMed Dutka TL, Lamb GD: Effect of carnosine on excitation-contraction coupling in mechanically-skinned rat skeletal muscle. J Muscle Res Cell Motil. 2004, 25: 203-213.CrossRefPubMed
28.
go back to reference Lamont C, Miller DJ: Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle. J Physiol. 1992, 454: 421-434.PubMedCentralCrossRefPubMed Lamont C, Miller DJ: Calcium sensitizing action of carnosine and other endogenous imidazoles in chemically skinned striated muscle. J Physiol. 1992, 454: 421-434.PubMedCentralCrossRefPubMed
29.
go back to reference Batrukova MA, Rubtsov AM: Histidine-containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels. Biochim Biophys Acta. 1997, 1324: 142-150. 10.1016/S0005-2736(96)00216-7.CrossRefPubMed Batrukova MA, Rubtsov AM: Histidine-containing dipeptides as endogenous regulators of the activity of sarcoplasmic reticulum Ca-release channels. Biochim Biophys Acta. 1997, 1324: 142-150. 10.1016/S0005-2736(96)00216-7.CrossRefPubMed
30.
go back to reference Roberts PR, Zaloga GP: Cardiovascular Effects of Carnosine. Biochem Mosc. 2000, 65: 856-861. Roberts PR, Zaloga GP: Cardiovascular Effects of Carnosine. Biochem Mosc. 2000, 65: 856-861.
31.
go back to reference Katz AM: Contractile proteins of the heart. Physiol Rev. 1970, 50: 63-158.PubMed Katz AM: Contractile proteins of the heart. Physiol Rev. 1970, 50: 63-158.PubMed
32.
go back to reference Westerblad H, Allen DG: The influence of intracellular pH on contraction, relaxation and [Ca2+] in intact single fibres from mouse muscle. J Physiol. 1993, 466: 611-628.PubMedCentralPubMed Westerblad H, Allen DG: The influence of intracellular pH on contraction, relaxation and [Ca2+] in intact single fibres from mouse muscle. J Physiol. 1993, 466: 611-628.PubMedCentralPubMed
33.
go back to reference Harris RC, Dunnett M, Greenhaff PL: Carnosine and Taurine contents in individual fibres of human vastuslateralis muscle. J Sport Sci. 1998, 16: 639-643. 10.1080/026404198366443.CrossRef Harris RC, Dunnett M, Greenhaff PL: Carnosine and Taurine contents in individual fibres of human vastuslateralis muscle. J Sport Sci. 1998, 16: 639-643. 10.1080/026404198366443.CrossRef
34.
go back to reference Sewell DA, Harris RC, Marlin DJ, Dunnett M: Estimation of the carnosine content of different fibre types in the middle gluteal muscle of the thoroughbred horse. J Physiol. 1992, 455: 447-453.PubMedCentralCrossRefPubMed Sewell DA, Harris RC, Marlin DJ, Dunnett M: Estimation of the carnosine content of different fibre types in the middle gluteal muscle of the thoroughbred horse. J Physiol. 1992, 455: 447-453.PubMedCentralCrossRefPubMed
35.
go back to reference Beltman JG, de Haan A, Haan H, Gerrits HL, van Mechelen W, Sargeant AJ: Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities. Eur J Appl Physiol. 2004, 92: 485-492.CrossRefPubMed Beltman JG, de Haan A, Haan H, Gerrits HL, van Mechelen W, Sargeant AJ: Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities. Eur J Appl Physiol. 2004, 92: 485-492.CrossRefPubMed
36.
go back to reference Kendrick IP, Kim HJ, Harris RC, Kim CK, Dang VH, Lam TQ, Bui TT, Wise JA: The effect of 4 weeks beta-alanine supplementation and isokinetic training on carnosine concentrations in type I and II human skeletal muscle fibres. Eur J Appl Physiol. 2009, 106: 131-138. 10.1007/s00421-009-0998-5.CrossRefPubMed Kendrick IP, Kim HJ, Harris RC, Kim CK, Dang VH, Lam TQ, Bui TT, Wise JA: The effect of 4 weeks beta-alanine supplementation and isokinetic training on carnosine concentrations in type I and II human skeletal muscle fibres. Eur J Appl Physiol. 2009, 106: 131-138. 10.1007/s00421-009-0998-5.CrossRefPubMed
Metadata
Title
β-alanine supplementation improves isometric endurance of the knee extensor muscles
Authors
Craig Sale
Chester A Hill
James Ponte
Roger C Harris
Publication date
01-12-2012
Publisher
BioMed Central
DOI
https://doi.org/10.1186/1550-2783-9-26

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