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Pediatric Rheumatology
Published in: Pediatric Rheumatology 1/2014

Open Access 01-12-2014 | Research

The involvement of the vasa vasorum in the development of vasculitis in animal model of Kawasaki disease

Authors: Akiko Hamaoka-Okamoto, Chinatsu Suzuki, Tomoyo Yahata, Kazuyuki Ikeda, Noriko Nagi-Miura, Naohito Ohno, Yoshinori Arai, Hideo Tanaka, Tetsuro Takamatsu, Kenji Hamaoka

Published in: Pediatric Rheumatology | Issue 1/2014

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Abstract

Background

Kawasaki Disease (KD) involves a diffuse and systemic vasculitis of unknown etiology that mainly affects infants and children. Although a considerable number of analyses of the clinical, histopathological and molecular biological details underlying the mechanism responsible for the development of coronary arterial lesions, it is still poorly understood.
The purpose of this study was to analyze the state of angiogenesis, vasculogenesis and the distribution of blood vessels using an animal model of KD like vasculitis. We investigated the involvement of the vasa vasorum from the adventitia in the vascular involvement and the development of the disease state by performing sequential histopathology, scanning electron microscopy (SEM) and micro computed tomography (CT) studies using a murine model of vasculitis induced by the Candida albicans water-soluble fraction (CAWS).

Methods

To prepare the animal model of KD like vasculitis, CAWS was intraperitoneally injected into C57BL/6N mice for five consecutive days as reported by Ohno et al. We observed the changes of the vasa vasorum at the aorta and the orifices of the coronary arteries by SEM and micro CT, and also compared the neovascularization at the media and adventitia of the aorta by an immunohistochemical analysis.

Results

As previously reported, obvious inflammation was detected two weeks after the injection of CAWS, and also intimal thickening was observed three weeks after the injection. We found that the vasa vasorum in the adventitia of the aorta was increased in the model mice. The vasa vasorum started increasing one week after the injection of CAWS, before any obvious vasculitis was microscopically detected.

Conclusion

The present results indicate that the vasculitis in Kawasaki disease starts as a disorder of the vasa vasorum.
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Metadata
Title
The involvement of the vasa vasorum in the development of vasculitis in animal model of Kawasaki disease
Authors
Akiko Hamaoka-Okamoto
Chinatsu Suzuki
Tomoyo Yahata
Kazuyuki Ikeda
Noriko Nagi-Miura
Naohito Ohno
Yoshinori Arai
Hideo Tanaka
Tetsuro Takamatsu
Kenji Hamaoka
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Pediatric Rheumatology / Issue 1/2014
Electronic ISSN: 1546-0096
DOI
https://doi.org/10.1186/1546-0096-12-12

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