Published in:
Open Access
01-11-2013 | Meeting abstract
PW02-036 - Thermosensitive CA2+ assay innate immune cells
Authors:
T Remijn, M Stoffels, A Simon, JW van der Meer
Published in:
Pediatric Rheumatology
|
Special Issue 1/2013
Login to get access
Excerpt
Temperature has immunomodulatory effects on innate immune cells: incubating monocytes at low temperatures for example increases pro-inflammatory responses after LPS-stimulation. The mechanisms mediating this effect are still unclear. The activity of several ion channels is sensitive to changes in temperature, leading to cytosolic calcium influx. Calcium is universally involved in cellular processes, its effects depending on the cellular context. Although not much is known about the role of calcium in immune cells, it has been shown that calcium is necessary for inflammasome assembly. Conventional methods of measuring calcium are not ideal to study the relation between temperature and intracellular calcium concentrations ([Ca2+]i). …
