Published in:
Open Access
01-12-2011 | Research
Construction and characterisation of a recombinant fowlpox virus that expresses the human papilloma virus L1 protein
Authors:
Carlo Zanotto, Eleana Pozzi, Sole Pacchioni, Massimiliano Bissa, Carlo De Giuli Morghen, Antonia Radaelli
Published in:
Journal of Translational Medicine
|
Issue 1/2011
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Abstract
Background
Human papilloma virus (HPV)-16 is the most prevalent high-risk mucosal genotype. Virus-like-particle (VLP)-based immunogens developed recently have proven to be successful as prophylactic HPV vaccines, but are still too expensive for developing countries. Although vaccinia viruses expressing the HPV-16 L1 protein (HPV-L1) have been studied, fowlpox-based recombinants represent efficient and safer vectors for immunocompromised hosts due to their ability to elicit a complete immune response and their natural host-range restriction to avian species.
Methods
A new fowlpox virus recombinant encoding HPV-L1 (FPL1) was engineered and evaluated for the correct expression of HPV-L1 in vitro, using RT-PCR, immunoprecipitation, Western blotting, electron microscopy, immunofluorescence, and real-time PCR assays.
Results
The FPL1 recombinant correctly expresses HPV-L1 in mammalian cells, which are non-permissive for the replication of this vector.
Conclusion
This FPL1 recombinant represents an appropriate immunogen for expression of HPV-L1 in human cells. The final aim is to develop a safe, immunogenic, and less expensive prophylactic vaccine against HPV.