Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2014

Open Access 01-12-2014 | Research

Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis

Authors: Yong Song, Li Xiao, Jing Fu, Wei Huang, Qiushi Wang, Xianghui Zhang, Shiyuan Yang

Published in: Reproductive Biology and Endocrinology | Issue 1/2014

Login to get access

Abstract

Background

The precise etiology of endometriosis is not fully understood; the involvement of stem cells theory is a new hypothesis. Related studies mainly focus on stemness-related genes, and pluripotency markers may play a role in the etiology of endometriosis. We aimed to analyze the transcription pluripotency factors sex-determining region Y-box 2 (SOX2), Nanog homeobox (NANOG), and octamer-binding protein 4 (OCT4) in the endometrium of reproductive-age women with and without ovarian endometriosis.

Methods

We recruited 26 women with laparoscopy-diagnosed ovarian endometriosis (endometriosis group) and 16 disease-free women (control group) to the study. Endometrial and endometriotic samples were collected. SOX2, NANOG, and OCT4 expression were analyzed with quantitative real-time polymerase chain reaction, western blotting, and immunohistochemistry.

Results

Compared to the control group, SOX2 mRNA and protein expression was significantly higher in the eutopic endometrium of participants in the endometriosis group. In the endometriosis group, SOX2 and NANOG mRNA and protein expression were significantly increased in ectopic endometrium compared with eutopic endometrium; there was a trend towards lower OCT4 mRNA expression and higher OCT4 protein expression in ectopic endometrium.

Conclusions

The transcription pluripotency factors SOX2 and NANOG were overexpression in ovarian endometriosis, their role in pathogenesis of endometriosis should be further studied.
Appendix
Available only for authorised users
Literature
1.
2.
Macer ML, Taylor HS: Endometriosis and infertility: a review of the pathogenesis and treatment of endometriosis-associated infertility. Obstet Gynecol Clin North Am. 2012, 39: 535-549. 10.1016/j.ogc.2012.10.002.PubMedCentralCrossRefPubMed
3.
Chan RW, Schwab KE, Gargett CE: Clonogenicity of human endometrial epithelial and stromal cells. Biol Reprod. 2004, 70: 1738-1750. 10.1095/biolreprod.103.024109.CrossRefPubMed
4.
Schwab KE, Chan RW, Gargett CE: Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle. Fertil Steril. 2005, 84: 1124-1130.CrossRefPubMed
5.
6.
Oliveira FR, Dela Cruz C, Del Puerto HL, Vilamil QT, Reis FM, Camargos AF: Stem cells: are they the answer to the puzzling etiology of endometriosis?. Histol Histopathol. 2012, 27: 23-29.PubMed
7.
Forte A, Schettino MT, Finicelli M, Cipollaro M, Colacurci N, Cobellis L, Galderisi U: Expression pattern of stemness-related genes in human endometrial and endometriotic tissues. Mol Med. 2009, 15: 392-401.PubMedCentralCrossRefPubMed
8.
Gotte M, Wolf M, Staebler A, Buchweitz O, Kiesel L, Schuring AN: Aberrant expression of the pluripotency marker SOX2 in endometriosis. Fertil Steril. 2011, 95: 338-341. 10.1016/j.fertnstert.2010.08.006.CrossRefPubMed
9.
Chang JH, Au HK, Lee WC, Chi CC, Ling TY, Wang LM, Kao SH, Huang YH, Tzeng CR: Expression of the pluripotent transcription factor OCT4 promotes cell migration in endometriosis. Fertil Steril. 2013, 99: 1332-1339. 10.1016/j.fertnstert.2012.11.033.CrossRefPubMed
10.
Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell-Badge R: Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev. 2003, 17: 126-140. 10.1101/gad.224503.PubMedCentralCrossRefPubMed
11.
Fang X, Yu W, Li L, Shao J, Zhao N, Chen Q, Ye Z, Lin SC, Zheng S, Lin B: ChIP-seq and functional analysis of the SOX2 gene in colorectal cancers. OMICS. 2010, 14: 369-384. 10.1089/omi.2010.0053.CrossRefPubMed
12.
Kehler J, Tolkunova E, Koschorz B, Pesce M, Gentile L, Boiani M, Lomeli H, Nagy A, McLaughlin KJ, Scholer HR, Tomilin A: Oct4 is required for primordial germ cell survival. EMBO Rep. 2004, 5: 1078-1083. 10.1038/sj.embor.7400279.PubMedCentralCrossRefPubMed
13.
Siu MK, Wong ES, Kong DS, Chan HY, Jiang L, Wong OG, Lam EW, Chan KK, Ngan HY, Le XF, Cheung AN: Stem cell transcription factor NANOG controls cell migration and invasion via dysregulation of E-cadherin and FoxJ1 and contributes to adverse clinical outcome in ovarian cancers. Oncogene. 2013, 32: 3500-3509. 10.1038/onc.2012.363.CrossRefPubMed
14.
Chiou SH, Wang ML, Chou YT, Chen CJ, Hong CF, Hsieh WJ, Chang HT, Chen YS, Lin TW, Hsu HS, Wu CW: Coexpression of Oct4 and Nanog enhances malignancy in lung adenocarcinoma by inducing cancer stem cell-like properties and epithelial-mesenchymal transdifferentiation. Cancer Res. 2010, 70: 10433-10444. 10.1158/0008-5472.CAN-10-2638.CrossRefPubMed
15.
Han X, Fang X, Lou X, Hua D, Ding W, Foltz G, Hood L, Yuan Y, Lin B: Silencing SOX2 induced mesenchymal epithelial transition and its expression predicts liver and lymph node metastasis of CRC patients. PLoS One. 2012, 7: e41335-10.1371/journal.pone.0041335.PubMedCentralCrossRefPubMed
16.
Noyes RW, Hertig AT, Rock J: Dating the endometrial biopsy. Am J Obstet Gynecol. 1975, 122: 262-263.PubMed
17.
Fong H, Hohenstein KA, Donovan PJ: Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cells. 2008, 26: 1931-1938. 10.1634/stemcells.2007-1002.CrossRefPubMed
18.
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007, 131: 861-872. 10.1016/j.cell.2007.11.019.CrossRefPubMed
19.
Rodda DJ, Chew JL, Lim LH, Loh YH, Wang B, Ng HH, Robson P: Transcriptional regulation of nanog by OCT4 and SOX2. J Biol Chem. 2005, 280: 24731-24737. 10.1074/jbc.M502573200.CrossRefPubMed
20.
Dai X, Ge J, Wang X, Qian X, Zhang C, Li X: OCT4 regulates epithelial-mesenchymal transition and its knockdown inhibits colorectal cancer cell migration and invasion. Oncol Rep. 2013, 29: 155-160.PubMed
21.
Zhou X, Zhou YP, Huang GR, Gong BL, Yang B, Zhang DX, Hu P, Xu SR: Expression of the stem cell marker, Nanog, in human endometrial adenocarcinoma. Int J Gynecol Pathol. 2011, 30: 262-270.PubMedCentralCrossRefPubMed
22.
Dmowski WP, Ding J, Shen J, Rana N, Fernandez BB, Braun DP: Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis. Hum Reprod. 2001, 16: 1802-1808. 10.1093/humrep/16.9.1802.CrossRefPubMed
23.
Taniguchi F, Kaponis A, Izawa M, Kiyama T, Deura I, Ito M, Iwabe T, Adonakis G, Terakawa N, Harada T: Apoptosis and endometriosis. Front Biosci (Elite Ed). 2011, 3: 648-662. 10.2741/e277.CrossRef
24.
Lin F, Lin P, Zhao D, Chen Y, Xiao L, Qin W, Li D, Chen H, Zhao B, Zou H, Zheng X, Yu X: Sox2 targets cyclinE, p27 and survivin to regulate androgen-independent human prostate cancer cell proliferation and apoptosis. Cell Prolif. 2012, 45: 207-216. 10.1111/j.1365-2184.2012.00812.x.CrossRefPubMed
25.
Feng R, Zhou S, Liu Y, Song D, Luan Z, Dai X, Li Y, Tang N, Wen J, Li L: Sox2 protects neural stem cells from apoptosis via up-regulating survivin expression. Biochem J. 2013, 450: 459-468. 10.1042/BJ20120924.CrossRefPubMed
26.
Zhang Y, Eades G, Yao Y, Li Q, Zhou Q: Estrogen receptor alpha signaling regulates breast tumor-initiating cells by down-regulating miR-140 which targets the transcription factor SOX2. J Biol Chem. 2012, 287: 41514-41522. 10.1074/jbc.M112.404871.PubMedCentralCrossRefPubMed
27.
Yang L, Luo L, Ji W, Gong C, Wu D, Huang H, Liu Q, Xia B, Hu G, Zhang W, Zhang Q, Liu J, Zhang W, Zhuang Z: Effect of low dose bisphenol A on the early differentiation of human embryonic stem cells into mammary epithelial cells. Toxicol Lett. 2013, 218: 187-193. 10.1016/j.toxlet.2013.01.026.CrossRefPubMed
28.
Borgfeldt C, Andolf E: Cancer risk after hospital discharge diagnosis of benign ovarian cysts and endometriosis. Acta Obstet Gynecol Scand. 2004, 83: 395-400. 10.1080/j.0001-6349.2004.00305.x.CrossRefPubMed
29.
Kobayashi H, Sumimoto K, Moniwa N, Imai M, Takakura K, Kuromaki T, Morioka E, Arisawa K, Terao T: Risk of developing ovarian cancer among women with ovarian endometrioma: a cohort study in Shizuoka, Japan. Int J Gynecol Cancer. 2007, 17: 37-43. 10.1111/j.1525-1438.2006.00754.x.CrossRefPubMed
30.
Ye F, Li Y, Hu Y, Zhou C, Chen H: Expression of Sox2 in human ovarian epithelial carcinoma. J Cancer Res Clin Oncol. 2011, 137: 131-137. 10.1007/s00432-010-0867-y.CrossRefPubMed
31.
Zhang J, Chang DY, Mercado-Uribe I, Liu J: Sex-determining region Y-box2 expression predicts poor prognosis in human ovarian carcinoma. Hum Pathol. 2012, 43: 1405-1412. 10.1016/j.humpath.2011.10.016.PubMedCentralCrossRefPubMed
Metadata
Title
Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis
Authors
Yong Song
Li Xiao
Jing Fu
Wei Huang
Qiushi Wang
Xianghui Zhang
Shiyuan Yang
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2014
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-12-42

Other articles of this Issue 1/2014

Reproductive Biology and Endocrinology 1/2014 Go to the issue

Research

A large, multicentre, observational, post-marketing surveillance study of the 2:1 formulation of follitropin alfa and lutropin alfa in routine clinical practice for assisted reproductive technology

Research

MicroRNA-34 family expression in bovine gametes and preimplantation embryos

Review

A critical review of bi-dimensional and three-dimensional ultrasound techniques to monitor follicle growth: do they help improving IVF outcome?

Research

Endometrial thickness affects the outcome of in vitro fertilization and embryo transfer in normal responders after GnRH antagonist administration

Research

Characterizing semen parameters and their association with reactive oxygen species in infertile men

Research

Evaluation of atorvastatin efficacy and toxicity on spermatozoa, accessory glands and gonadal hormones of healthy men: a pilot prospective clinical trial