Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2013

Open Access 01-12-2013 | Research

Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis

Authors: Rosella Locci, Michelle Nisolle, Stefano Angioni, Jean-Michel Foidart, Carine Munaut

Published in: Reproductive Biology and Endocrinology | Issue 1/2013

Login to get access

Abstract

Background

Endometrial cells, which are shed by retrograde menstruation, may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of the laminin gamma 2 chain (LAMC2) in the tissues of women with and without endometriosis.

Methods

Endometrial biopsy specimens were collected from healthy volunteers and from endometriosis patients. Biopsy specimens from the corresponding endometriotic lesions were also collected. The expression of laminin gamma 2 chain was evaluated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).

Results

Endometrial tissue from women with or without endometriosis showed constitutive expression of LAMC2 mRNA throughout the menstrual cycle. A higher mRNA level was observed in ectopic endometrium (Ec) from women with endometriosis compared with eutopic endometrium (Eu) from women with endometriosis. Immunohistochemistry revealed a varied pattern of laminin gamma 2 chain expression, with increased epithelial expression in eutopic endometrium from women with endometriosis compared with those without endometriosis.

Conclusions

The altered expression of laminin gamma 2 chain in eutopic endometrium from women with endometriosis may provide new opportunities for diagnosis and treatment.
Appendix
Available only for authorised users
Literature
1.
2.
3.
Rousselle P, Lunstrum G, Keene D, Burgeson R: Kalinin: an epithelium-specific basement membrane adhesion molecule that is a component of anchoring filaments. J Cell Biol. 1991, 114: 567-576. 10.1083/jcb.114.3.567.CrossRefPubMed
4.
Carter WG, Ryan MC, Gahr PJ: Epiligrin, a New cell adhesion ligand for integrin alpha 3 beta 1 in epithelial basement membranes. Cell. 1991, 65: 599-610. 10.1016/0092-8674(91)90092-D.CrossRefPubMed
5.
Kikkawa Y, Umeda M, Miyazaki K: Marked Stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. J Biochem. 1994, 116: 862-869.PubMed
6.
Rousselle P, Aumailley M: Kalinin Is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and Has a different requirement for integrin receptors. J Cell Biol. 1994, 125: 205-214. 10.1083/jcb.125.1.205.CrossRefPubMed
7.
Fukushima Y, Ohnishi T, Arita N, Hayakawa T, Sekiguchi K: Integrin Alpha3beta1-mediated interaction with laminin-5 stimulates adhesion, migration and invasion of malignant glioma cells. Int J Cancer. 1998, 76: 63-72. 10.1002/(SICI)1097-0215(19980330)76:1<63::AID-IJC11>3.0.CO;2-H.CrossRefPubMed
8.
Pyke C, Salo S, Ralfkiaer E, Romer J, Dano K, Tryggvason K: Laminin-5 Is a marker of invading cancer cells in some human carcinomas and is co expressed with the receptor for urokinase plasminogen activator in budding cancer cells in colon adenocarcinomas. Cancer Res. 1995, 55: 4132-4139.PubMed
9.
Sordat I, Bosman F, Dorta G, Rousselle P, Aberdam D, Blum A, Sordat B: Differential expression of laminin-5 subunits and integrin receptors in human colorectal neoplasia. J Pathol. 1998, 185: 44-52. 10.1002/(SICI)1096-9896(199805)185:1<44::AID-PATH46>3.0.CO;2-A.CrossRefPubMed
10.
Noyes RW, Hertig AT, Rock J: Dating the endometrial biopsy. Am J Obstet Gynecol. 1975, 122: 262-263.PubMed
11.
Tsatsaris V, Goffin F, Munaut C, Brichant J, Pignon M, Noel A, Schaaps J, Cabrol D, Frankenne F, Foidart J: Overexpression of the soluble vascular endothelial growth factor receptor in preeclamptic patients: pathophysiological consequences. J Clin Endocrinol Metab. 2003, 88: 5555-5563. 10.1210/jc.2003-030528.CrossRefPubMed
12.
Goffin F, Munaut C, Frankenne F, Perrier D’hauterive S, Beliard A, Fridman V, Nervo P, Colige A, Foidart J: Expression pattern of metalloproteinases and tissue inhibitors of matrix-metalloproteinases in cycling human endometrium. Biol Reprod. 2003, 69: 976-984. 10.1095/biolreprod.103.015933.CrossRefPubMed
13.
Nisolle M, Alvarez M, Colombo M, Foidart J: Pathogenesis of endometriosis. Gynecol Obstet Fertil. 2007, 35: 898-903. 10.1016/j.gyobfe.2007.07.021.CrossRefPubMed
14.
Beliard A, Donnez J, Nisolle M, Foidart J: Localization of laminin, fibronectin, E-cadherin, and integrins in endometrium and endometriosis. Fertil Steril. 1997, 67: 266-272. 10.1016/S0015-0282(97)81909-7.CrossRefPubMed
15.
Harrington D, Lessey B, Rai V, Bergqvist A, Kennedy S, Manek S, Barlow D, Mardon H: Tenascin is differentially expressed in endometrium and endometriosis. J Pathol. 1999, 187: 242-248. 10.1002/(SICI)1096-9896(199901)187:2<242::AID-PATH221>3.0.CO;2-T.CrossRefPubMed
16.
Sundqvist J, Andersson K, Scarselli G, Gemzell-Danielsson K, Lalitkumar P: Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: a link to the aetiology of endometriosis. Hum Reprod. 2012, 27: 2737-2746. 10.1093/humrep/des220.CrossRefPubMed
17.
Umezawa M, Saito Y, Tanaka-Hattori N, Takeda K, Ihara T, Sugamata M: Expression profile of extracellular matrix and adhesion molecules in the development of endometriosis in a mouse model. Reprod Sci. 2012, 19: 1365-1372. 10.1177/1933719112450340.CrossRefPubMed
18.
Greenbaum D, Colangelo C, Williams K, Gerstein M: Comparing Protein abundance and mRNA expression levels on a genomic scale. Genome Biol. 2003, 4: 117-10.1186/gb-2003-4-9-117.PubMedCentralCrossRefPubMed
19.
Vogel C, Marcotte E: Insights Into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat Rev Genet. 2012, 13: 227-232.PubMedCentralPubMed
20.
Pan Q, Luo X, Toloubeydokhti T, Chegini N: The Expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression. Mol Hum Reprod. 2007, 13: 797-806. 10.1093/molehr/gam063.CrossRefPubMed
21.
Gilabert-Estelles J, Braza-Boils A, Ramon L, Zorio E, Medina P, Espana F, Estelles A: Role Of microRNAs in gynecological pathology. Curr Med Chem. 2012, 19: 2406-2413. 10.2174/092986712800269362.CrossRefPubMed
22.
Giannelli G, Sgarra C, Di Naro E, Lavopa C, Angelotti U, Tartagni M, Simone O, Trerotoli P, Antonaci S, Loverro G: Endometriosis is characterized by an impaired localization of laminin-5 and alpha3 beta1 integrin receptor. Int J Gynecol Cancer. 2007, 17: 242-247. 10.1111/j.1525-1438.2006.00750.x.CrossRefPubMed
23.
24.
Martin K, Kwan C, Nagasaki K, Zhang X, O’hare M, Kaelin C, Burgeson R, Pardee A, Sager R: Down-regulation of laminin-5 in breast carcinoma cells. Mol Med. 1998, 4: 602-613.PubMedCentralPubMed
25.
Soini Y, Maatta M, Salo S, Tryggvason K, Autio-Harmainen H: Expression of the laminin gamma 2 chain in pancreatic adenocarcinoma. J Pathol. 1996, 180: 290-294. 10.1002/(SICI)1096-9896(199611)180:3<290::AID-PATH661>3.0.CO;2-6.CrossRefPubMed
26.
Koshikawa N, Moriyama K, Takamura H, Mizushima H, Nagashima Y, Yanoma S, Miyazaki K: Overexpression of laminin gamma 2 chain monomer in invading gastric carcinoma cells. Cancer Res. 1999, 59: 5596-5601.PubMed
27.
Kagesato Y, Mizushima H, Koshikawa N, Kitamura H, Hayashi H, Ogawa N, Tsukuda M, Miyazaki K: Sole Expression of laminin gamma 2 chain in invading tumor cells and its association with stromal fibrosis in lung adenocarcinomas. Jpn J Cancer Res. 2001, 92: 184-192. 10.1111/j.1349-7006.2001.tb01081.x.CrossRefPubMed
28.
Giannelli G, Fransvea E, Bergamini C, Marinosci F, Antonaci S: Laminin-5 chains are expressed differentially in metastatic and nonmetastatic hepatocellular carcinoma. Clin Cancer Res. 2003, 9: 3684-3691.PubMed
29.
Gilles C, Polette M, Coraux C, Tournier J, Meneguzzi G, Munaut C, Volders L, Rousselle P, Birembaut P, Foidart J: Contribution of MT1-MMP and of human laminin-5 gamma 2 chain degradation to mammary epithelial cell migration. J Cell Sci. 2001, 114: 2967-2976.PubMedCentralPubMed
30.
Kuratomi Y, Sato S, Monji M, Shimazu R, Tanaka G, Yokogawa K, Inoue A, Inokuchi A, Katayama M: Serum concentrations of laminin gamma 2 fragments in patients with head and neck squamous cell carcinoma. Head Neck. 2008, 30: 1058-1063. 10.1002/hed.20838.CrossRefPubMed
Metadata
Title
Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis
Authors
Rosella Locci
Michelle Nisolle
Stefano Angioni
Jean-Michel Foidart
Carine Munaut
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2013
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-11-94

Other articles of this Issue 1/2013

Reproductive Biology and Endocrinology 1/2013 Go to the issue

Research

Fertility in a high-altitude environment is compromised by luteal dysfunction: the relative roles of hypoxia and oxidative stress

Short communication

Occurrence of multi-oocyte follicles in aquaporin 8-deficient mice

Research

Protective effects of nuclear factor erythroid 2-related factor 2 on whole body heat stress-induced oxidative damage in the mouse testis

Research

Castration-induced testosterone deficiency increases fasting glucose associated with hepatic and extra-hepatic insulin resistance in adult male rats

Research

Effects of corticotrophin releasing hormone (CRH) on cell viability and differentiation in the human BeWo choriocarcinoma cell line: a potential syncytialisation inducer distinct from cyclic adenosine monophosphate (cAMP)

Research

Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation