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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2013

Open Access 01-12-2013 | Research

Differential expression of beta-catenin and dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study

Authors: Zhan Zhang, He Li, Linlin Zhang, Liting Jia, Peng Wang

Published in: Reproductive Biology and Endocrinology | Issue 1/2013

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Abstract

Background

Beta-catenin is a key nuclear effector of Wnt signaling which could be antagonized by dickkopf-1(DKK1). Beta-catenin and DKK1 are involved in a variety of biological processes; however, their expression in the placenta with severe preeclampsia (PE) has not been elucidated. This study was aimed to detect the localization and compare the expression of beta-catenin and DKK1 in normal and preeclamptic placenta.

Methods

Sixty pregnant women who underwent cesarean section were enrolled in this study, including 30 healthy pregnant women in the control group and 30 preeclamptic women in the severe PE group. Real-time polymerase chain reaction (real-time-PCR) and western blot were employed to detect the beta-catenin and DKK1 mRNA and protein expression levels, respectively, and their locations were evaluated by immunohistochemistry (IHC).

Results

Our results indicated that beta-catenin and DKK1 were expressed predominantly in the syncytiotrophoblast and the extravillous trophoblast (EVT). The beta-catenin mRNA and protein expressions were significantly decreased, whereas the DKK1 significantly increased in preeclamptic placental tissues compared to normal placental controls.

Conclusions

In conclusion, decreased beta-catenin expression, as well as DKK1 over-expression might be associated with the process of the pathogenesis of PE. Further studies would elucidate their exact roles in the pathogenesis of PE.
Appendix
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Literature
1.
Redman CW, Sargent IL: Latest advances in understanding preeclampsia. Science. 2005, 308: 1592-1594. 10.1126/science.1111726.CrossRefPubMed
2.
LaMarca BD, Gilbert J, Granger JP: Recent progress toward the understanding of the pathophysiology of hypertension during preeclampsia. Hypertension. 2008, 51: 982-988. 10.1161/HYPERTENSIONAHA.107.108837.PubMedCentralCrossRefPubMed
3.
Red-Horse K, Zhou Y, Genbacev O, Prakobphol A, Foulk R, McMaster M, Fisher SJ: Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface. J Clin Invest. 2004, 114: 744-754.PubMedCentralCrossRefPubMed
4.
Clevers H: Wnt/beta-catenin signaling in development and disease. Cell. 2006, 127: 469-480. 10.1016/j.cell.2006.10.018.CrossRefPubMed
5.
6.
7.
Willert K, Jones KA: Wnt signaling: is the party in the nucleus?. Genes Dev. 2006, 20: 1394-1404. 10.1101/gad.1424006.CrossRefPubMed
8.
Mao B, Wu W, Davidson G, Marhold J, Li M, Mechler BM, Delius H, Hoppe D, Stannek P, Walter C: Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling. Nature. 2002, 417: 664-667. 10.1038/nature756.CrossRefPubMed
9.
Niehrs C: Function and biological roles of the Dickkopf family of Wnt modulators. Oncogene. 2006, 25: 7469-7481. 10.1038/sj.onc.1210054.CrossRefPubMed
10.
Sonderegger S, Husslein H, Leisser C, Knofler M: Complex expression pattern of Wnt ligands and frizzled receptors in human placenta and its trophoblast subtypes. Placenta. 2007, 28 (Suppl A): S97-S102.PubMedCentralCrossRefPubMed
11.
Pollheimer J, Loregger T, Sonderegger S, Saleh L, Bauer S, Bilban M, Czerwenka K, Husslein P, Knofler M: Activation of the canonical wingless/T-cell factor signaling pathway promotes invasive differentiation of human trophoblast. Am J Pathol. 2006, 168: 1134-1147. 10.2353/ajpath.2006.050686.PubMedCentralCrossRefPubMed
12.
Fitzgerald JS, Germeyer A, Huppertz B, Jeschke U, Knofler M, Moser G, Scholz C, Sonderegger S, Toth B, Markert UR: Governing the invasive trophoblast: current aspects on intra- and extracellular regulation. Am J Reprod Immunol. 2010, 63: 492-505. 10.1111/j.1600-0897.2010.00824.x.CrossRefPubMed
13.
Sonderegger S, Pollheimer J, Knofler M: Wnt signalling in implantation, decidualisation and placental differentiation–review. Placenta. 2010, 31: 839-847. 10.1016/j.placenta.2010.07.011.PubMedCentralCrossRefPubMed
14.
ACOG practice bulletin: Diagnosis and management of preeclampsia and eclampsia. Number 33, January 2002. Obstet Gynecol. 2002, 99: 159-167. 10.1016/S0029-7844(01)01747-1.CrossRef
15.
Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.CrossRefPubMed
16.
Angers S, Moon RT: Proximal events in Wnt signal transduction. Nat Rev Mol Cell Biol. 2009, 10: 468-477.CrossRefPubMed
17.
Paul S, Dey A: Wnt signaling and cancer development: therapeutic implication. Neoplasma. 2008, 55: 165-176.PubMed
18.
Lantsov D, Meirmanov S, Nakashima M, Kondo H, Saenko V, Naruke Y, Namba H, Ito M, Abrosimov A, Lushnikov E: Cyclin D1 overexpression in thyroid papillary microcarcinoma: its association with tumour size and aberrant beta-catenin expression. Histopathology. 2005, 47: 248-256. 10.1111/j.1365-2559.2005.02218.x.CrossRefPubMed
19.
20.
Kinzler KW, Vogelstein B: Lessons from hereditary colorectal cancer. Cell. 1996, 87: 159-170. 10.1016/S0092-8674(00)81333-1.CrossRefPubMed
21.
Grisaru-Granovsky S, Maoz M, Barzilay O, Yin YJ, Prus D, Bar-Shavit R: Protease activated receptor-1, PAR1, promotes placenta trophoblast invasion and beta-catenin stabilization. J Cell Physiol. 2009, 218: 512-521. 10.1002/jcp.21625.CrossRefPubMed
22.
van der Horst PH, Wang Y, van der Zee M, Burger CW, Blok LJ: Interaction between sex hormones and WNT/beta-catenin signal transduction in endometrial physiology and disease. Mol Cell Endocrinol. 2012, 358: 176-184. 10.1016/j.mce.2011.06.010.CrossRefPubMed
23.
Cerdeira AS, Karumanchi SA: Angiogenic factors in preeclampsia and related disorders. Cold Spring Harb Perspect Med. 2012, 2 (11): pii: a006585-10.1101/cshperspect.a006585. ISSN: 2157-1422CrossRef
24.
Cattelino A, Liebner S, Gallini R, Zanetti A, Balconi G, Corsi A, Bianco P, Wolburg H, Moore R, Oreda B: The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. J Cell Biol. 2003, 162: 1111-1122. 10.1083/jcb.200212157.PubMedCentralCrossRefPubMed
25.
Wang H, Wen S, Bunnett NW, Leduc R, Hollenberg MD, MacNaughton WK: Proteinase-activated receptor-2 induces cyclooxygenase-2 expression through beta-catenin and cyclic AMP-response element-binding protein. J Biol Chem. 2008, 283: 809-815. 10.1074/jbc.M703021200.CrossRefPubMed
26.
Aguilera O, Fraga MF, Ballestar E, Paz MF, Herranz M, Espada J, Garcia JM, Munoz A, Esteller M, Gonzalez-Sancho JM: Epigenetic inactivation of the Wnt antagonist DICKKOPF-1 (DKK-1) gene in human colorectal cancer. Oncogene. 2006, 25: 4116-4121. 10.1038/sj.onc.1209439.CrossRefPubMed
27.
Lee TL, Yeh J, Van Waes C, Chen Z: Epigenetic modification of SOCS-1 differentially regulates STAT3 activation in response to interleukin-6 receptor and epidermal growth factor receptor signaling through JAK and/or MEK in head and neck squamous cell carcinomas. Mol Cancer Ther. 2006, 5: 8-19.CrossRefPubMed
28.
Liu Y, Kodithuwakku SP, Ng PY, Chai J, Ng EH, Yeung WS, Ho PC, Lee KF: Excessive ovarian stimulation up-regulates the Wnt-signaling molecule DKK1 in human endometrium and may affect implantation: an in vitro co-culture study. Hum Reprod. 2010, 25: 479-490. 10.1093/humrep/dep429.CrossRefPubMed
29.
Sonderegger S, Haslinger P, Sabri A, Leisser C, Otten JV, Fiala C, Knofler M: Wingless (Wnt)-3A induces trophoblast migration and matrix metalloproteinase-2 secretion through canonical Wnt signaling and protein kinase B/AKT activation. Endocrinology. 2010, 151: 211-220. 10.1210/en.2009-0557.PubMedCentralCrossRefPubMed
30.
Peng S, Li J, Miao C, Jia L, Hu Z, Zhao P, Zhang Y, Chen Q, Duan E: Dickkopf-1 secreted by decidual cells promotes trophoblast cell invasion during murine placentation. Reproduction. 2008, 135: 367-375. 10.1530/REP-07-0191.CrossRefPubMed
31.
von Dadelszen P, Magee LA, Roberts JM: Subclassification of preeclampsia. Hypertens Pregnancy. 2003, 22: 143-148. 10.1081/PRG-120021060.CrossRefPubMed
32.
Huppertz B: Placental origins of preeclampsia: challenging the current hypothesis. Hypertension. 2008, 51: 970-975. 10.1161/HYPERTENSIONAHA.107.107607.CrossRefPubMed
Metadata
Title
Differential expression of beta-catenin and dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study
Authors
Zhan Zhang
He Li
Linlin Zhang
Liting Jia
Peng Wang
Publication date
01-12-2013
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2013
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-11-17

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