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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2012

Open Access 01-12-2012 | Research

Dynamics of microRNAs in bull spermatozoa

Authors: Aruna Govindaraju, Alper Uzun, LaShonda Robertson, Mehmet O Atli, Abdullah Kaya, Einko Topper, Elizabeth A Crate, James Padbury, Andy Perkins, Erdogan Memili

Published in: Reproductive Biology and Endocrinology | Issue 1/2012

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Abstract

Background

MicroRNAs are small non-coding RNAs that regulate gene expression and thus play important roles in mammalian development. However, the comprehensive lists of microRNAs, as well as, molecular mechanisms by which microRNAs regulate gene expression during gamete and embryo development are poorly defined. The objectives of this study were to determine microRNAs in bull sperm and predict their functions.

Methods

To accomplish our objectives we isolated miRNAs from sperm of high and low fertility bulls, conducted microRNA microarray experiments and validated expression of a panel of microRNAs using real time RT-PCR. Bioinformatic approaches were carried out to identify regulated targets.

Results

We demonstrated that an abundance of microRNAs were present in bovine spermatozoa, however, only seven were differentially expressed; hsa-aga-3155, -8197, -6727, -11796, -14189, -6125, -13659. The abundance of miRNAs in the spermatozoa and the differential expression in sperm from high vs. low fertility bulls suggests that the miRNAs possibly play important functions in the regulating mechanisms of bovine spermatozoa.

Conclusion

Identification of specific microRNAs expressed in spermatozoa of bulls with different fertility phenotypes will help better understand mammalian gametogenesis and early development.
Appendix
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Metadata
Title
Dynamics of microRNAs in bull spermatozoa
Authors
Aruna Govindaraju
Alper Uzun
LaShonda Robertson
Mehmet O Atli
Abdullah Kaya
Einko Topper
Elizabeth A Crate
James Padbury
Andy Perkins
Erdogan Memili
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2012
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-10-82

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