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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2012

Open Access 01-12-2012 | Research

Human prolyl hydroxylase expression in uterine leiomyoma during the menstrual cycle

Authors: Masaaki Iwahashi, Yasuteru Muragaki, Kazuhiko Ino

Published in: Reproductive Biology and Endocrinology | Issue 1/2012

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Abstract

Background

To investigate the role of prolyl hydroxylase (PH), a key enzyme of collagen synthesis, in human uterine leiomyoma, PH expression was determined in the normal uterine myometrium and the leiomyoma tissues during the menstrual cycle.

Methods

The tissues were obtained from 40 regularly cycling women (aged 29 to 53 yr) who were undergoing abdominal hysterectomy for symptomatic uterine leiomyoma. Immunohistochemistry for human PH with specific monoclonal antibody was used for analysis.

Results

Immunohistochemical staining for PH revealed intense staining of leiomyoma cells in the uterine leiomyoma throughout the menstrual cycle, as compared with the adjacent normal myometrium. In the secretory phase, weak or no immunostaining for PH was detected in the normal myometrial tissues.

Conclusions

These results suggest that increased expression of PH might play an role in the physiology of uterine leiomyoma during the menstrual cycle.
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Metadata
Title
Human prolyl hydroxylase expression in uterine leiomyoma during the menstrual cycle
Authors
Masaaki Iwahashi
Yasuteru Muragaki
Kazuhiko Ino
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2012
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-10-111

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