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Molecular Cancer
Published in: Molecular Cancer 1/2006

Open Access 01-12-2006 | Research

Proteomic alteration in gastic adenocarcinomas from Japanese patients

Authors: Takahiro Yoshihara, Yoshito Kadota, Yoshiyuki Yoshimura, Yutaka Tatano, Naohiro Takeuchi, Hiroshi Okitsu, Atsushi Umemoto, Takashi Yamauchi, Kohji Itoh

Published in: Molecular Cancer | Issue 1/2006

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Abstract

Background

Gastric adenocarcinomas comprise one of the common types of cancers in Asian countries including Japan. Comprehensive protein profiling of paired surgical specimens of primary gastric adenocarcinomas and nontumor mucosae derived from Japanese patients was carried out by means of two-dimensional gel electrophoresis (2D-EP) and liquid chromatography-electrospray ionic tandem mass spectrometry (LC-ESI-MS) to establish gastric cancer-specific proteins as putative clinical biomarkers and molecular targets for chemotherapy.

Results

Relatively common alterations in protein expression were revealed in the tumor tissues. Increases in manganese dismutase and nonhistone chromosomal protein HMG-1 (HMG-1) were observed, while decreases in carbonic anhydrases I and II, glutatione-S-transferase and foveolin precursor (gastrokine-1) (FOV), an 18-kDa stomach-specific protein with putative tumor suppressor activity, were detected. RT-PCR analysis also revealed significant down-regulation of FOV mRNA expression in tumor tissues.

Conclusion

A possible pathological role for down-regulation of FOV in gastric carcinogenesis was demonstrated. Evaluation of the specific decreases in gene and protein expression of FOV in patients may be utilized as clinical biomarkers for effective diagnosis and assessment of gastric cancer.
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Metadata
Title
Proteomic alteration in gastic adenocarcinomas from Japanese patients
Authors
Takahiro Yoshihara
Yoshito Kadota
Yoshiyuki Yoshimura
Yutaka Tatano
Naohiro Takeuchi
Hiroshi Okitsu
Atsushi Umemoto
Takashi Yamauchi
Kohji Itoh
Publication date
01-12-2006
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2006
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/1476-4598-5-75

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