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Open Access 01-12-2006 | Research

Effect of ret/PTC 1 rearrangement on transcription and post-transcriptional regulation in a papillary thyroid carcinoma model

Authors: Susanne Cahill, Paul Smyth, Stephen P Finn, Karen Denning, Richard Flavin, Esther M O'Regan, Jinghuan Li, Astrid Potratz, Simone M Guenther, Richard Henfrey, John J O'Leary, Orla Sheils

Published in: Molecular Cancer | Issue 1/2006

Abstract

Background

microRNAs (miRNAs) are a group of non-coding single stranded RNAs measuring approximately 22 nt in length that have been found to control cell growth, differentiation and apoptosis. miRNAs negatively regulate their target genes and recently have been implicated in tumourigenesis. Furthermore, miRNA expression profiling correlates with various cancers, with these genes thought to act as both tumour suppressors and oncogenes. ret/PTC 1 is an oncogene with constitutive kinase activity implicated in the development of papillary thyroid carcinoma (PTC). This rearrangement leads to aberrant MAPK activation that is implicated in PTC tumourigenesis.

Aim

The aim of this study was to identify the effect that ret/PTC 1 has on transcription and post-transcriptional regulation in PTC by using DNA microarray and microRNA analysis.

Results

DNA microarray analysis revealed a group of genes differentially expressed between normal thyroid cell lines and those harbouring a ret/PTC 1 rearrangement.

Furthermore, a unique miRNA expression signature differentiated between PTC cell lines with ret/PTC 1 and a normal thyroid cell line. 21 miRNAs showed significant overexpression and 14 miRNAs showed underexpression in these cell lines when compared to normal thyroid. Several of these up/down regulated miRNAs may be involved in PTC pathogenesis.

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Title: Effect of ret/PTC 1 rearrangement on transcription and post-transcriptional regulation in a papillary thyroid carcinoma model
Authors: Susanne Cahill
Paul Smyth
Stephen P Finn
Karen Denning
Richard Flavin
Esther M O'Regan
Jinghuan Li
Astrid Potratz
Simone M Guenther
Richard Henfrey
John J O'Leary
Orla Sheils
Publication date: 01-12-2006
Publisher: BioMed Central
Published in: Molecular Cancer / Issue 1/2006
Electronic ISSN: 1476-4598
DOI: https://doi.org/10.1186/1476-4598-5-70

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