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Molecular Cancer
Published in: Molecular Cancer 1/2011

Open Access 01-12-2011 | Research

The human RECQ1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferation

Authors: Ramiro Mendoza-Maldonado, Valentina Faoro, Sailesh Bajpai, Matteo Berti, Federico Odreman, Marco Vindigni, Tamara Ius, Abdollah Ghasemian, Serena Bonin, Miran Skrap, Giorgio Stanta, Alessandro Vindigni

Published in: Molecular Cancer | Issue 1/2011

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Abstract

Background

RecQ helicases play an essential role in the maintenance of genome stability. In humans, loss of RecQ helicase function is linked with predisposition to cancer and/or premature ageing. Current data show that the specific depletion of the human RECQ1 helicase leads to mitotic catastrophe in cancer cells and inhibition of tumor growth in mice.

Results

Here, we show that RECQ1 is highly expressed in various types of solid tumors. However, only in the case of brain gliomas, the high expression of RECQ1 in glioblastoma tissues is paralleled by a lower expression in the control samples due to the poor expression of RECQ1 in non-dividing tissues. This conclusion is validated by immunohistochemical analysis of a tissue microarray containing 63 primary glioblastomas and 19 perilesional tissue samples, as control. We also show that acute depletion of RECQ1 by RNAi results in a significant reduction of cellular proliferation, perturbation of S-phase progression, and spontaneous γ-H2AX foci formation in T98G and U-87 glioblastoma cells. Moreover, RECQ1 depleted T98G and U-87 cells are hypersensitive to HU or temozolomide treatment.

Conclusions

Collectively, these results indicate that RECQ1 has a unique and important role in the maintenance of genome integrity. Our results also suggest that RECQ1 might represent a new suitable target for anti cancer therapies aimed to arrest cell proliferation in brain gliomas.
Appendix
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Metadata
Title
The human RECQ1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferation
Authors
Ramiro Mendoza-Maldonado
Valentina Faoro
Sailesh Bajpai
Matteo Berti
Federico Odreman
Marco Vindigni
Tamara Ius
Abdollah Ghasemian
Serena Bonin
Miran Skrap
Giorgio Stanta
Alessandro Vindigni
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Molecular Cancer / Issue 1/2011
Electronic ISSN: 1476-4598
DOI
https://doi.org/10.1186/1476-4598-10-83

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