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Cancer Cell International
Published in: Cancer Cell International 1/2008

Open Access 01-12-2008 | Primary research

Release of volatile organic compounds (VOCs) from the lung cancer cell line CALU-1 in vitro

Authors: Wojciech Filipiak, Andreas Sponring, Tomas Mikoviny, Clemens Ager, Jochen Schubert, Wolfram Miekisch, Anton Amann, Jakob Troppmair

Published in: Cancer Cell International | Issue 1/2008

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Abstract

Background

The aim of this work was to confirm the existence of volatile organic compounds (VOCs) specifically released or consumed by lung cancer cells.

Methods

50 million cells of the human non-small cell lung cancer (NSCLC) cell line CALU-1 were incubated in a sealed fermenter for 4 h or over night (18 hours). Then air samples from the headspace of the culture vessel were collected and preconcentrated by adsorption on solid sorbents with subsequent thermodesorption and analysis by means of gas chromatography mass spectrometry (GC-MS). Identification of altogether 60 compounds in GCMS measurement was done not only by spectral library match, but also by determination of retention times established with calibration mixtures of the respective pure compounds.

Results

The results showed a significant increase in the concentrations of 2,3,3-trimethylpentane, 2,3,5-trimethylhexane, 2,4-dimethylheptane and 4-methyloctane in the headspace of CALU-1 cell culture as compared to medium controls after 18 h. Decreased concentrations after 18 h of incubation were found for acetaldehyde, 3-methylbutanal, butyl acetate, acetonitrile, acrolein, methacrolein, 2-methylpropanal, 2-butanone, 2-methoxy-2-methylpropane, 2-ethoxy-2-methylpropane, and hexanal.

Conclusion

Our findings demonstrate that certain volatile compounds can be cancer-cell derived and thus indicative of the presence of a tumor, whereas other compounds are not released but seem to be consumed by CALU-1 cells.
Appendix
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Literature
1.
Amann A, Smith D, (eds): Breath Analysis for Clinical Diagnosis and Therapeutic Monitoring. 2005, Singapore: World Scientific
2.
Amann A, Spanel P, Smith D: Breath analysis: the approach towards clinical applications. Mini Rev Med Chem. 2007, 7 (2): 115-129.CrossRefPubMed
3.
Schubert J, Miekisch W, Nöldge-Schomburg G: VOC breath markers in critically ill patients: potentials and limitations. Breath Analysis for Clinical Diagnosis and Therapeutic Monitoring. Edited by: Amann A, Smith D. 2005, Singapore: World Scientific, 267-292.CrossRef
4.
Risby T: Current status of clinical breath analysis. Breath Analysis for Clinical Diagnosis and Therapeutic Monitoring. Edited by: Amann A, Smith D. 2005, Singapore: World Scientific, 251-265.CrossRef
5.
Schubert JK, Miekisch W, Geiger K, Noldge-Schomburg GF: Breath analysis in critically ill patients: potential and limitations. Expert Rev Mol Diagn. 2004, 4 (5): 619-629.CrossRefPubMed
6.
Davies S, Spanel P, Smith D: A new 'online' method to measure increased exhaled isoprene in end-stage renal failure. Nephrol Dial Transplant. 2001, 16 (4): 836-839.CrossRefPubMed
7.
Davies S, Spanel P, Smith D: Quantitative analysis of ammonia on the breath of patients in end-stage renal failure. Kidney Int. 1997, 52 (1): 223-228.CrossRefPubMed
8.
Davies SJ, Spanel P, Smith D: Quantitative analysis of metabolites on the breath of patients in renal failure. Journal of the American Society of Nephrology. 1996, 7 (9): A0352-A0352.
9.
Perri F, Marras RM, Ricciardi R, Quitadamo M, Andriulli A: 13C-breath tests in hepatology (cytosolic liver function). Eur Rev Med Pharmacol Sci. 2004, 8 (1): 47-49.PubMed
10.
Candelli M, Armuzzi A, Nista EC, Fini L, Gasbarrini G, Gasbarrini A: 13C-methacetin breath test for monitoring hepatic function in cirrhotic patients before and after liver transplantation. Aliment Pharmacol Ther. 2004, 19 (2): 243-CrossRefPubMed
11.
Phillips M, Altorki N, Austin JH, Cameron RB, Cataneo RN, Greenberg J, Kloss R, Maxfield RA, Munawar MI, Pass HI, Rashid A, Rom WN, Schmitt P: Prediction of lung cancer using volatile biomarkers in breath. Cancer Biomark. 2007, 3 (2): 95-109.PubMed
12.
Phillips M, Cataneo RN, Cummin AR, Gagliardi AJ, Gleeson K, Greenberg J, Maxfield RA, Rom WN: Detection of lung cancer with volatile markers in the breath. Chest. 2003, 123 (6): 2115-2123.CrossRefPubMed
13.
Phillips M, Cataneo RN, Ditkoff BA, Fisher P, Greenberg J, Gunawardena R, Kwon CS, Rahbari-Oskoui F, Wong C: Volatile markers of breast cancer in the breath. Breast J. 2003, 9 (3): 184-191.CrossRefPubMed
14.
Wehinger A, Schmid A, Mechtcheriakov S, Ledochowski M, Grabmer C, Gastl G, Amann A: Lung cancer detection by proton transfer reaction mass spectrometric analysis of human breath gas. Int J Mass Spec. 2007, 265: 49-59.CrossRef
15.
Machado RF, Laskowski D, Deffenderfer O, Burch T, Zheng S, Mazzone PJ, Mekhail T, Jennings C, Stoller JK, Pyle J, Duncan J, Dweik RA, Erzurum SC: Detection of lung cancer by sensor array analyses of exhaled breath. Am J Respir Crit Care Med. 2005, 171 (11): 1286-1291.PubMedCentralCrossRefPubMed
16.
Di Natale C, Macagnano A, Martinelli E, Paolesse R, D'Arcangelo G, Roscioni C, Finazzi-Agro A, D'Amico A: Lung cancer identification by the analysis of breath by means of an array of non-selective gas sensors. Biosens Bioelectron. 2003, 18 (10): 1209-1218.CrossRefPubMed
17.
Poli D, Carbognani P, Corradi M, Goldoni M, Acampa O, Balbi B, Bianchi L, Rusca M, Mutti A: Exhaled volatile organic compounds in patients with non-small cell lung cancer: cross sectional and nested short-term follow-up study. Respir Res. 2005, 6 (1): 71-PubMedCentralCrossRefPubMed
18.
Amann A, Telser S, Hofer L, Schmid A, Hinterhuber H: Exhaled breath as a biochemical probe during sleep. Breath Analysis for Clinical Diagnosis and Therapeutic Monitoring. Edited by: Amann A, Smith D. 2005, Singapore: World Scientific, 305-316.CrossRef
19.
Smith D, Spanel P: On-line determination of the deuterium abundance in breath water vapour by flowing afterglow mass spectrometry with applications to measurements of total body water. Rapid Commun Mass Spectrom. 2001, 15 (1): 25-32.CrossRefPubMed
20.
Smith D, Spanel P: On-line determination of the deuterium abundance in breath water vapour by flowing afterglow mass spectrometry, FA-MS, with applications to measurements of total body water. Rapid Commun Mass Spectrom. 2001, 15 (1): 25-32.CrossRefPubMed
21.
Smith D, Wang TS, Spanel P: On-line, simultaneous quantification of ethanol, some metabolites and water vapour in breath following the ingestion of alcohol. Physiological Measurement. 2002, 23 (3): 477-489.CrossRefPubMed
22.
Smith D, Wang T, Sule-Suso J, Spanel P, Haj AE: Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry. Rapid Commun Mass Spectrom. 2003, 17 (8): 845-850.CrossRefPubMed
23.
Homann N: Alcohol and upper gastrointestinal tract cancer: the role of local acetaldehyde production. Addiction biology. 2001, 6 (4): 309-323.CrossRefPubMed
24.
Balicki D: Moving forward in human mammary stem cell biology and breast cancer prognostication using ALDH1. Cell Stem Cell. 2007, 1 (5): 485-487.CrossRefPubMed
25.
Chang JW, Jeon HB, Lee JH, Yoo JS, Chun JS, Kim JH, Yoo YJ: Augmented expression of peroxiredoxin I in lung cancer. Biochem Biophys Res Commun. 2001, 289 (2): 507-512.CrossRefPubMed
26.
Patel M, Lu L, Zander DS, Sreerama L, Coco D, Moreb JS: ALDH1A1 and ALDH3A1 expression in lung cancers: correlation with histologic type and potential precursors. Lung Cancer. 2008, 59 (3): 340-349.CrossRefPubMed
Metadata
Title
Release of volatile organic compounds (VOCs) from the lung cancer cell line CALU-1 in vitro
Authors
Wojciech Filipiak
Andreas Sponring
Tomas Mikoviny
Clemens Ager
Jochen Schubert
Wolfram Miekisch
Anton Amann
Jakob Troppmair
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2008
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/1475-2867-8-17

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