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Cancer Cell International
Published in: Cancer Cell International 1/2012

Open Access 01-12-2012 | Primary research

Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells

Authors: Wissem Bhouri, Jihed Boubaker, Ines Skandrani, Kamel Ghedira, Leila Chekir Ghedira

Published in: Cancer Cell International | Issue 1/2012

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Abstract

Background

The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.

Methods

We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.

Results

The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.

Conclusion

In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.
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Metadata
Title
Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells
Authors
Wissem Bhouri
Jihed Boubaker
Ines Skandrani
Kamel Ghedira
Leila Chekir Ghedira
Publication date
01-12-2012
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2012
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/1475-2867-12-26

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