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BMC Complementary Medicine and Therapies

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Open Access 01-12-2014 | Research article

The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats

Authors: Ze-Jun Huo, Quan Li, Gui-Hua Tian, Chang-Man Zhou, Xiao-Hong Wei, Chun-Shui Pan, Lei Yang, Yan Bai, You-Yi Zhang, Ke He, Chuan-She Wang, Zhi-Gang Li, Jing-Yan Han

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

Abstract

Background

The purpose of this study was to investigate the inhibitory effects of long-term electroacupuncture at BaiHui (DU20) and ZuSanLi (ST36) on cardiovascular remodeling in spontaneously hypertensive rats (SHR) and underlying mechanisms.

Methods

6-weeks-old SHR or Wistar male rats were randomly, divided into 6 groups: the control group (SHR/Wistar), the non-acupoint electroacupuncture stimulation group (SHR-NAP/Wistar-NAP) and the electroacupuncture stimulation at DU20 and ST36 group (SHR-AP/Wistar-AP), 24 rats in each group. Rats were treated with or without electroacupuncture at DU20 and ST36, once every other day for a period of 8 weeks. The mean arterial pressure (MAP) was measured once every 2 weeks. By the end of the 8th week, the left ventricular structure and function were assessed by echocardiography. The content of angiotensin II (Ang II), endothelin-1 (ET-1) and nitric oxide (NO) in the plasma was determined using enzyme-linked immunosorbent assay. Histological studies on the heart and the ascending aorta were performed. The expression of angiotensin II type 1 receptor (AT1R), endothelin-1 type A receptor (ETAR), eNOS and iNOS in rat myocardium and ascending aorta was investigated by Western blotting.

Results

The MAP in SHR increased linearly over the observation period and significantly reduced following electroacupuncture as compared with sham control SHR rats, while no difference in MAP was observed in Wistar rats between electroacupuncture and sham control. The aortic wall thickness, cardiac hypertrophy and increased collagen level in SHR were attenuated by long term electroacupuncture. The content of Ang II, ET-1 in the plasma decreased, but the content of NO increased after electroacupuncture stimulation in SHR. Long term electroacupuncture significantly inhibited the expression of AT1R, ETAR and iNOS, whereas increased eNOS expression, in myocardium and ascending aorta of SHR.

Conclusions

The long term electroacupuncture stimulation at DU20 and ST36 relieves the increased MAP and cardiovascular abnormality in both structure and function in SHR, this beneficial action is most likely mediated via modulation of AT1R-AT1R-ET-1-ETAR and NOS/NO pathway.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/118/prepub

Title: The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats
Authors: Ze-Jun Huo
Quan Li
Gui-Hua Tian
Chang-Man Zhou
Xiao-Hong Wei
Chun-Shui Pan
Lei Yang
Yan Bai
You-Yi Zhang
Ke He
Chuan-She Wang
Zhi-Gang Li
Jing-Yan Han
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-14-118

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The ameliorating effects of long-term electroacupuncture on cardiovascular remodeling in spontaneously hypertensive rats

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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