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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2012

Open Access 01-12-2012 | Research article

Pleurotus giganteus (Berk.) Karunarathna & K.D. Hyde: Nutritional value and in vitro neurite outgrowth activity in rat pheochromocytoma cells

Authors: Chia-Wei Phan, Wei-Lun Wong, Pamela David, Murali Naidu, Vikineswary Sabaratnam

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

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Abstract

Background

Drugs dedicated to alleviate neurodegenerative diseases like Parkinson’s and Alzheimer’s have always been associated with debilitating side effects. Medicinal mushrooms which harness neuropharmacological compounds offer a potential possibility for protection against such diseases. Pleurotus giganteus (formerly known as Panus giganteus) has been consumed by the indigenous people in Peninsular Malaysia for many years. Domestication of this wild mushroom is gaining popularity but to our knowledge, medicinal properties reported for this culinary mushroom are minimal.

Methods

The fruiting bodies P. giganteus were analysed for its nutritional values. Cytotoxicity of the mushroom’s aqueous and ethanolic extracts towards PC12, a rat pheochromocytoma cell line was assessed by using 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Neurite outgrowth stimulation assay was carried out with nerve growth factor (NGF) as control. To elucidate signaling mechanisms involved by mushroom extract-induced neurite outgrowth, treatment of specific inhibitor for MEK/ERK and PI3K signalling pathway was carried out.

Results

The fruiting bodies of P. giganteus were found to have high carbohydrate, dietary fibre, potassium, phenolic compounds and triterpenoids. Both aqueous and ethanolic extracts induced neurite outgrowth of PC12 cells in a dose- and time-dependant manner with no detectable cytotoxic effect. At day 3, 25 μg/ml of aqueous extract and 15 μg/ml of ethanolic extract showed the highest percentage of neurite-bearing cells, i.e. 31.7 ± 1.1% and 33.3 ± 0.9%; respectively. Inhibition treatment results suggested that MEK/ERK and PI3K/Akt are responsible for neurite outgrowth of PC12 cells stimulated by P. giganteus extract. The high potassium content (1345.7 mg/100 g) may be responsible for promoting neurite extension, too.

Conclusions

P. giganteus contains bioactive compounds that mimic NGF and are responsible for neurite stimulation. Hence, this mushroom may be developed as a nutraceutical for the mitigation of neurodegenerative diseases.
Appendix
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Literature
1.
Shulman JM, Jager PLD: Evidence for a common pathway linking neurodegenerative diseases. Nat Gent. 2009, 41 (12): 1261-1262. 10.1038/ng1209-1261.CrossRef
2.
Parkinson J: An essay on the shaking palsy. J Neuropsychiatry Clin Neurosci. 2002, 14 (2): 223-222. 10.1176/appi.neuropsych.14.2.223.CrossRefPubMed
3.
Miyasaki JM, Martin W, Suchowersky O, Weiner WJ, Lang AE: Practice parameter: initiation of treatment for Parkinson’s disease: an evidence-based review: report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology. 2002, 58: 11-17. 10.1212/WNL.58.1.11.CrossRefPubMed
4.
Suchowersky O, Gronseth G, Perlmutter J, Reich S, Zesiewicz T, Weiner WJ: Practice parameter: neuroprotective strategies and alternative therapies for Parkinson disease (an evidence-based review): report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology. 2006, 66 (7): 976-982. 10.1212/01.wnl.0000206363.57955.1b.CrossRefPubMed
5.
Wong KH, Sabaratnam V, Abdullah N, Naidu M, Keynes R: Activity of aqueous extracts of lions mane mushroom Hericium erinaceus (Bull.: Fr.) Pers. (Aphyllophoromycetideae) on the neural cell line NG108-15. Int J Med Mushrooms. 2007, 9 (1): 57-65. 10.1615/IntJMedMushr.v9.i1.70.CrossRef
6.
Wong KH, Naidu M, David RP, Abdulla MA, Abdullah N, Kuppusamy UR, Sabaratnam V: Functional recovery enhancement following injury to rodent peroneal nerve by lions mane mushroom, Hericium erinaceus (Bull.: Fr.) Pers. (Aphyllophoromycetideae). Int J Med Mushrooms. 2009, 11 (3): 225-236. 10.1615/IntJMedMushr.v11.i3.20.CrossRef
7.
Wong KH, Naidu M, David P, Abdulla MA, Abdullah N, Kuppusamy UR, Sabaratnam V: Peripheral nerve regeneration following crush injury to rat peroneal nerve by aqueous extract of medicinal mushroom Hericium Erinaceus (Bull.: Fr) Pers. (Aphyllophoromycetideae). Evid-Based Compl Alt. 2011, 2011 (Article ID 580752): 10-10.1093/ecam/neq062.
8.
Eik LF, Naidu M, David P, Wong KH, Tan YS, Sabaratnam V: Lignosus rhinocerus (Cooke) Ryvarden: A medicinal mushroom that stimulates neurite outgrowth in PC-12 cells. Evid-Based Compl Alt. 2012, 2012 (Article ID 320308): 7-10.1155/2012/320308.
9.
Milbrandt J: Nerve growth factor rapidly induces c-fos mRNA in PC12 rat pheochromocytoma cells. Proc Natl Acad Sci USA. 1986, 83 (13): 4789-4793. 10.1073/pnas.83.13.4789.CrossRefPubMedPubMedCentral
10.
Nishina A, Kimura H, Sekiguchi A, Fukumoto R, Nakajima S, Furukawa S: Lysophosphatidylethanolamine in Grifola frondosa as a neurotrophic activator via activation of MAPK. J Lipid Res. 2006, 47 (7): 1434-1443. 10.1194/jlr.M600045-JLR200.CrossRefPubMed
11.
Mori K, Obara Y, Hirota M, Azumi Y, Kinugasa S, Inatomi S, Nakahata N: Nerve growth factor-inducing activity of Hericium erinaceus in 1321 N1 human astrocytoma cells. Biol Pharm Bull. 2008, 31 (9): 1727-1732. 10.1248/bpb.31.1727.CrossRefPubMed
12.
Lee SS, Chang YS, Noraswati MNR: Utilization of macrofungi by some indigenous communities for food and medicine in Peninsular Malaysia. Forest Ecol Manag. 2009, 257 (10): 2062-2065. 10.1016/j.foreco.2008.09.044.CrossRef
13.
Deng W, Li T, Chen Z, Wu L, Yang W, Zhang G: A critical note on the scientific name of the cultivated edible fungus, Zhudugu. Acta Edulis Fungi. 2006, 13 (3): 75-79.
14.
Corner E: The Agaric genera Lentinus, Panus, and Pleurotus with particular reference to Malaysian species. 1981, Vaduz: J Cramer
15.
Pegler D: The classification of the genus Lentinus Fr. (Basidiomycota). Kavaka. 1975, 3: 11-20.
16.
Abdullah S, Abdullah N, Sabaratnam V, Grand E: Basidiomycota: Distribution and new records of Lentinus. Malaysian Fungal Diversity. Edited by: John EBG, Hyde KD, Sabaratnam V. 2007, Mushroom Research Centre, University of Malaya and Ministry of Natural Resources and Environment, 83-93.
17.
Karunarathna SC, Yang ZL, Ko TWK, Vellinga EC, Zhao RL, Bahkali AH, Chukeatirote E, Degreef J, Callac P, Hyde KD: Lentinus giganteus revisited: new collections from Sri Lanka and Thailand. Mycotaxon. 2012, 118: 57-71. 10.5248/118.57.CrossRef
18.
Walsh K, Megyesi J, Hammond R: Human central nervous system tissue culture: a historical review and examination of recent advances. Neurobiol Dis. 2005, 18 (1): 2-18. 10.1016/j.nbd.2004.09.002.CrossRefPubMed
19.
Radio NM, Mundy WR: Developmental neurotoxicity testing in vitro: models for assessing chemical effects on neurite outgrowth. Neurotoxicology. 2008, 29 (3): 361-376. 10.1016/j.neuro.2008.02.011.CrossRefPubMed
20.
Carmeliet P: Blood vessels and nerves: common signals, pathways and diseases. Nat Rev Genet. 2003, 4 (9): 710-720. 10.1038/nrg1158.CrossRefPubMed
21.
Das KP, Freudenrich TM, Mundy WR: Assessment of PC12 cell differentiation and neurite growth: a comparison of morphological and neurochemical measures. Neurotoxicol Teratol. 2004, 26 (3): 397-406. 10.1016/j.ntt.2004.02.006.CrossRefPubMed
22.
Greene LA, Tischler AS: PC12 pheochromocytoma cells in neurobiological research. Adv Cel. Neurobiology. 1982, 3: 373-414.CrossRef
23.
Wong WL, Abdulla MA, Chua KH, Rani U, Tan YS, Sabaratnam V: Hepatoprotective effects of Panus giganteus (Berk.) Corner against thioacetamide (TAA)-induced liver injury in rats. Evid-Based Compl Alt. 2012, 2012 (Article ID 170303): 10-10.1155/2012/170303.
24.
Weyermann J, Lochmann D, Zimmer A: A practical note on the use of cytotoxicity assays. Int J Pharmaceut. 2005, 288 (2): 369-376. 10.1016/j.ijpharm.2004.09.018.CrossRef
25.
Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells. Dev Growth Differ. 2006, 48 (8): 513-523. 10.1111/j.1440-169X.2006.00889.x.CrossRefPubMed
26.
Wu H, Ichikawa S, Tani C, Zhu B, Tada M, Shimoishi Y, Murata Y, Nakamura Y: Docosahexaenoic acid induces ERK1/2 activation and neuritogenesis via intracellular reactive oxygen species production in human neuroblastoma SH-SY5Y cells. Biochim Biophys Acta. 2009, 1791 (1): 8-16. 10.1016/j.bbalip.2008.10.004.CrossRefPubMed
27.
Zhang W, Liu HT: MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 2002, 12 (1): 9-18. 10.1038/sj.cr.7290105.CrossRefPubMed
28.
Omar NAM, Abdullah N, Kuppusamy UR, Abdulla MA, Sabaratnam V: Nutritional composition, antioxidant activities, and antiulcer potential of Lentinus squarrosulus (Mont.) mycelia extract. Evid-Based Compl Alt. 2011, 2011 (Article ID 539356): 8-10.1155/2011/539356.
29.
Bonatti M, Karnopp P, Soares HM, Furlan SA: Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. Food Chem. 2004, 88: 425-428. 10.1016/j.foodchem.2004.01.050.CrossRef
30.
Barros L, Cruz T, Baptista P, Estevinho LM, Ferreira ICFR: Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food Chem Toxicol. 2008, 46 (8): 2742-2747. 10.1016/j.fct.2008.04.030.CrossRefPubMed
31.
Reis FS, Barros L, Martins A, Ferreira ICFR: Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study. Food Chem Toxicol. 2012, 50 (2): 191-197. 10.1016/j.fct.2011.10.056.CrossRefPubMed
32.
Wang D, Sakoda A, Suzuki M: Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresource Technol. 2001, 78: 293-300. 10.1016/S0960-8524(01)00002-5.CrossRef
33.
Goyal R, Grewal RB, Goyal RK: Nutritional attributes of Agaricus bisporus and Pleurotus sajor caju mushrooms. Nutr Health. 2006, 18: 179-184. 10.1177/026010600601800209.CrossRefPubMed
34.
Alam N, Amin R, Khan A, Ara I, Shim MJ, Lee MW, Lee TS: Nutritional analysis of cultivated mushrooms in Bangladesh- Pleurotus ostreatus, Pleurotus sajor-caju, Pleurotus florida and Calocybe indica. Mycobiology. 2008, 36 (4): 228-232. 10.4489/MYCO.2008.36.4.228.CrossRefPubMedPubMedCentral
35.
Caglarirmak N: The nutrients of exotic mushrooms (Lentinula edodes and Pleurotus species) and an estimated approach to the volatile compounds. Food Chem. 2007, 105: 1188-1194. 10.1016/j.foodchem.2007.02.021.CrossRef
36.
Mattila P, Salo-Vaananen P, Konko K, Aro H, Jalava T: Basic composition and amino acid contents of mushrooms cultivated in Finland. J Agr Food Chem. 2002, 50: 6419-6422. 10.1021/jf020608m.CrossRef
37.
Manzi P, Aguzzi A, Pizzoferrato L: Nutritional value of mushrooms widely consumed in Italy. Food Chem. 2001, 73 (3): 321-325. 10.1016/S0308-8146(00)00304-6.CrossRef
38.
Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983, 65: 55-63. 10.1016/0022-1759(83)90303-4.CrossRefPubMed
39.
Cheung WMW, Hui WS, Chu PWK, Chiu SW, Ip NY: Ganoderma extract activates MAP kinases and induces the neuronal differentiation of rat pheochromocytoma PC12 cells. FEBS Lett. 2000, 486 (3): 291-296. 10.1016/S0014-5793(00)02317-6.CrossRefPubMed
40.
Zhang X, Ip FCF, Zhang D, Chen L, Zhang W, Li Y, Ip NY, Ye W: Triterpenoids with neurotrophic activity from Ganoderma lucidum. Nat Prod Res. 2011, 25 (17): 1607-1613. 10.1080/14786419.2010.496367.CrossRefPubMed
41.
Kalac P: Chemical composition and nutritional value of European species of wild growing mushrooms: A review. Food Chem. 2009, 113 (1): 9-16. 10.1016/j.foodchem.2008.07.077.CrossRef
42.
Cohen-Cory S, Dreyfus CF, Black IB: NGF and excitatory neurotransmitters morphogenesis of cultured cerebellar regulate survival and Purkinje cells. J Neurosci. 1991, 11 (2): 462-471.PubMed
43.
Wong KH, Sabaratnam V, Abdullah N, Kuppusamy UR, Naidu M: Effects of cultivation techniques and processing on antimicrobial and antioxidant activities of Hericium erinaceus (Bull.: Fr.) Pers. extracts. Food Technol Biotech. 2009, 47 (1): 47-55.
44.
Tsukamoto S, Macabalang AD, Nakatani K, Obara Y, Nakahata N, Ohta T: Tricholomalides A-C, new neurotrophic diterpenes from the mushroom Tricholoma sp. J Nat Prod. 2003, 66 (12): 1578-1581. 10.1021/np030140x.CrossRefPubMed
45.
Qi J, Ojika M, Sakagami Y: Termitomycesphins A–D, novel neuritogenic cerebrosides from the edible Chinese mushroom Termitomyces albuminosus. Tetrahedron. 2000, 56 (32): 5835-5841. 10.1016/S0040-4020(00)00548-2.CrossRef
46.
Qi J, Ojika M, Sakagami Y: Neuritogenic cerebrosides from an edible Chinese mushroom. Part 2: Structures of two additional termitomycesphins and activity enhancement of an inactive cerebroside by hydroxylation. Bioorgan Med Chem. 2001, 9 (8): 2171-2177. 10.1016/S0968-0896(01)00125-0.CrossRef
47.
Lee IK, Yun BS, Han GH, Cho D, Kim Y, Yoo I: Dictyoquinazols A, B, and C, new neuroprotective compounds from the mushroom Dictyophora indusiata. J Nat Prod. 2002, 65 (12): 1769-1772. 10.1021/np020163w.CrossRefPubMed
48.
Kim JH, Ha H, Lee M, Kang J, Kim H, Lee S, Pyun K, Shim I: Effect of Tremella fuciformis on the neurite outgrowth of PC12h cells and the improvement of memory in rats. Biol Pharm Bull. 2007, 30 (4): 708-714. 10.1248/bpb.30.708.CrossRefPubMed
49.
Tsuji M, Inanami O, Kuwabara M: Induction of neurite outgrowth in PC12 Cells by α-phenyl-N-tert- butylnitron through activation of protein kinase C and the Ras-extracellular signal-regulated kinase pathway. J Biol Chem. 2001, 276 (35): 32779-32785. 10.1074/jbc.M101403200.CrossRefPubMed
50.
Sweatt JD: The neuronal MAP kinase cascade: a biochemical signal integration system subserving synaptic plasticity and memory. J Neurochem. 2001, 76 (1): 1-10.CrossRefPubMed
51.
Kim Y, Seger R, Suresh Babu C, Hwang S, Yoo Y: A positive role of the PI3-K/Akt signaling pathway in PC12 cell differentiation. Mol Cells. 2004, 18 (3): 353-359.PubMed
52.
Naidu M, David RP, Asher R, Fawcett J: Expression of Akt and MAPK in the normal and regenerating peripheral nerves and their dorsal root ganglia. Malaysian Journal of Biochemistry and Molecular Biology. 2009, 17: 16-19.
Metadata
Title
Pleurotus giganteus (Berk.) Karunarathna & K.D. Hyde: Nutritional value and in vitro neurite outgrowth activity in rat pheochromocytoma cells
Authors
Chia-Wei Phan
Wei-Lun Wong
Pamela David
Murali Naidu
Vikineswary Sabaratnam
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-12-102

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