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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2010

Open Access 01-12-2010 | Research article

Curcumin supplementation could improve diabetes-induced endothelial dysfunction associated with decreased vascular superoxide production and PKC inhibition

Authors: Sirada Rungseesantivanon, Naris Thenchaisri, Preecha Ruangvejvorachai, Suthiluk Patumraj

Published in: BMC Complementary Medicine and Therapies | Issue 1/2010

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Abstract

Background

Curcumin, an Asian spice and food-coloring agent, is known for its anti-oxidant properties. We propose that curcumin can improve diabetes-induced endothelial dysfunction through superoxide reduction.

Methods

Diabetes (DM) was induced in rats by streptozotocin (STZ). Daily curcumin oral feeding was started six weeks after the STZ injection. Twelve weeks after STZ injection, mesenteric arteriolar responses were recorded in real time using intravital fluorescence videomicroscopy. Superoxide and vascular protein kinase C (PKC-βII) were examined by hydroethidine and immunofluorescence, respectively.

Results

The dilatory response to acetylcholine (ACh) significantly decreased in DM arterioles as compared to control arterioles. There was no difference among groups when sodium nitroprusside (SNP) was used. ACh responses were significantly improved by both low and high doses (30 and 300 mg/kg, respectively) of curcumin supplementation. An oxygen radical-sensitive fluorescent probe, hydroethidine, was used to detect intracellular superoxide anion (O2 ●-) production. O2 ●- production was markedly increased in DM arterioles, but it was significantly reduced by supplementation of either low or high doses of curcumin. In addition, with a high dose of curcumin, diabetes-induced vascular PKC-βII expression was diminished.

Conclusion

Therefore, it is suggested that curcumin supplementation could improve diabetes-induced endothelial dysfunction significantly in relation to its potential to decrease superoxide production and PKC inhibition.
Appendix
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Metadata
Title
Curcumin supplementation could improve diabetes-induced endothelial dysfunction associated with decreased vascular superoxide production and PKC inhibition
Authors
Sirada Rungseesantivanon
Naris Thenchaisri
Preecha Ruangvejvorachai
Suthiluk Patumraj
Publication date
01-12-2010
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2010
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-10-57

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