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BMC Oral Health
Published in: BMC Oral Health 1/2015

Open Access 01-12-2015 | Research article

Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis

Authors: Satosu Onozawa, Yuichiro Kikuchi, Kazuko Shibayama, Eitoyo Kokubu, Masaaki Nakayama, Tetsuyoshi Inoue, Keisuke Nakano, Yukinaga Shibata, Naoya Ohara, Koji Nakayama, Kazuyuki Ishihara, Toshiyuki Kawakami, Hiromasa Hasegawa

Published in: BMC Oral Health | Issue 1/2015

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Abstract

Background

Porphyromonas gingivalis has been implicated as a major pathogen in the development and progression of chronic periodontitis. P. gingivalis biofilm formation in the subgingival crevice plays an important role in the ability of the bacteria to tolerate stress signals outside the cytoplasmic membrane. Some bacteria use a distinct subfamily of sigma factors to regulate their extracytoplasmic functions (the ECF subfamily). The objective of this study was to determine if P. gingivalis ECF sigma factors affect P. gingivalis biofilm formation.

Methods

To elucidate the role of ECF sigma factors in P. gingivalis, chromosomal mutants carrying a disruption of each ECF sigma factor-encoding gene were constructed. Bacterial growth curves were measured by determining the turbidity of bacterial cultures. The quantity of biofilm growing on plates was evaluated by crystal violet staining.

Results

Comparison of the growth curves of wild-type P. gingivalis strain 33277 and the ECF mutants indicated that the growth rate of the mutants was slightly lower than that of the wild-type strain. The PGN_0274- and PGN_1740-defective mutants had increased biofilm formation compared with the wild-type (p < 0.001); however, the other ECF sigma factor mutants or the complemented strains did not enhance biofilm formation.

Conclusion

These results suggest that PGN_0274 and PGN_1740 play a key role in biofilm formation by P. gingivalis.
Appendix
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Metadata
Title
Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis
Authors
Satosu Onozawa
Yuichiro Kikuchi
Kazuko Shibayama
Eitoyo Kokubu
Masaaki Nakayama
Tetsuyoshi Inoue
Keisuke Nakano
Yukinaga Shibata
Naoya Ohara
Koji Nakayama
Kazuyuki Ishihara
Toshiyuki Kawakami
Hiromasa Hasegawa
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2015
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/1472-6831-15-4

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