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BMC Oral Health
Published in: BMC Oral Health 1/2014

Open Access 01-12-2014 | Research article

Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study

Authors: Andrea Pilloni, Giorgio Pompa, Matteo Saccucci, Gabriele Di Carlo, Lia Rimondini, Marina Brama, Blerina Zeza, Francesca Wannenes, Silvia Migliaccio

Published in: BMC Oral Health | Issue 1/2014

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Abstract

Background

Nano-hydroxyapatite (nHA) is a potential ideal biomaterial for bone regeneration. However, studies have yet to characterize the behavior of human osteoblasts derived from alveolar bone on nHA. Thus, the aim of the present study was to evaluate the influence of nHA on the adhesion, proliferation and differentiation of these alveolar bone-derived cells.

Methods

Primary human alveolar osteoblasts were collected from the alveolar ridge of a male periodontal patient during osseous resective surgery and grown on culture plates coated with either polylysine or polylysine with nano-hydroxyapatite (POL/nHA) composite. The cells were grown and observed for 14 days, and then assessed for potential modifications to osteoblasts homeostasis as evaluated by quantitative reverse transcriptase-polymerase chain reaction (real time RT-PCR), scanning electron microscopy and atomic force microscopy.

Results

Real time PCR revealed a significant increase in the expression of the selected markers of osteoblast differentiation (bone morphogenetic protein (BMP)-2,-5,-7, ALP, COLL-1A2, OC, ON) in cells grown on the POL/nHA substrate. In addition, as compared with the POL surface, cells grown on the POL/nHA substrate demonstrated better osteoconductive properties, as demonstrated by the increase in adhesion and spreading, likely as a result of the increased surface roughness of the composite.

Conclusions

The increased expression of BMPs and osteoinductive biomarkers suggest that nano-hydroxyapatite may stimulate the proliferation and differentiation of local alveolar osteoblasts and thus encourage bone regeneration at sites of alveolar bone regeneration.
Appendix
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Metadata
Title
Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study
Authors
Andrea Pilloni
Giorgio Pompa
Matteo Saccucci
Gabriele Di Carlo
Lia Rimondini
Marina Brama
Blerina Zeza
Francesca Wannenes
Silvia Migliaccio
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2014
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/1472-6831-14-22

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