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BMC Urology

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Open Access 01-12-2007 | Research article

Molecular and cellular characterization of ABCG2 in the prostate

Authors: Laura E Pascal, Asa J Oudes, Timothy W Petersen, Young Ah Goo, Laura S Walashek, Lawrence D True, Alvin Y Liu

Published in: BMC Urology | Issue 1/2007

Abstract

Background

Identification and characterization of the prostate stem cell is important for understanding normal prostate development and carcinogenesis. The flow cytometry-based side population (SP) technique has been developed to isolate putative adult stem cells in several human tissue types including the prostate. This phenotype is mainly mediated by the ATP-binding cassette membrane transporter ABCG2.

Methods

Immunolocalization of ABCG2 was performed on normal prostate tissue obtained from radical prostatectomies. Normal human prostate SP cells and ABCG2⁺ cells were isolated and gene expression was determined with DNA array analysis and RT-PCR. Endothelial cells were removed by pre-sorting with CD31.

Results

ABCG2 positive cells were localized to the prostate basal epithelium and endothelium. ABCG2⁺ cells in the basal epithelium constituted less than 1% of the total basal cell population. SP cells constituted 0.5–3% of the total epithelial fraction. The SP transcriptome was essentially the same as ABCG2⁺ and both populations expressed genes indicative of a stem cell phenotype, however, the cells also expressed many genes in common with endothelial cells.

Conclusion

These results provide gene expression profiles for the prostate SP and ABCG2⁺ cells that will be critical for studying normal development and carcinogenesis, in particular as related to the cancer stem cell concept.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2490/7/6/prepub

Title: Molecular and cellular characterization of ABCG2 in the prostate
Authors: Laura E Pascal
Asa J Oudes
Timothy W Petersen
Young Ah Goo
Laura S Walashek
Lawrence D True
Alvin Y Liu
Publication date: 01-12-2007
Publisher: BioMed Central
Published in: BMC Urology / Issue 1/2007
Electronic ISSN: 1471-2490
DOI: https://doi.org/10.1186/1471-2490-7-6

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Springer Medicine

Molecular and cellular characterization of ABCG2 in the prostate

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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