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BMC Urology

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Open Access 01-12-2012 | Research article

Expression of apoptosis-regulating genes in the rat prostate following botulinum toxin type a injection

Authors: Tiago Gorgal, Ana Charrua, João F Silva, António Avelino, Paulo Dinis, Francisco Cruz

Published in: BMC Urology | Issue 1/2012

Abstract

Background

Onabotulinumtoxin A (OnabotA) injection has been investigated as a novel treatment for benign prostatic enlargement caused by benign prostatic hyperplasia. An OnabotA - induced volume reduction caused by sympathetic fibers impairment has been proposed as a potential mechanism of action. Our aim was to investigate the expression of apoptosis-regulating proteins in the rat prostate following OnabotA intraprostatic injection.

Methods

Adult Wistar rats were injected in the ventral lobes of the prostate with 10 U of OnabotA or saline. A set of OnabotA-injected animals was further treated with 0.5 mg/kg of phenylephrine (PHE) subcutaneously daily. All animals were sacrificed after 1 week and had their prostates harvested. Immunohistochemical staining was performed for Bax, Bcl-xL and caspase-3 proteins and visualized by the avidin-biotin method. The optical density of the glandular cells was also determined, with measurement of differences between average optical densities for each group.

Results

Saline-treated animals showed intense epithelial staining for Bcl-xL and a faint labelling for both Bax and Caspase-3. OnabotA-treated rats showed a reduced epithelial staining of Bcl-xL and a consistently increased Bax and Caspase-3 staining when compared with saline-treated animals. PHE-treated animals showed a stronger Bcl-xL staining and reduced staining of both Bax and Caspase-3 when compared to the OnabotA group. Mean signal intensity measurements for each immunoreaction confirmed a significant decrease of the signal intensity for Bcl-xL and a significant increase of the signal intensity for Bax and Caspase 3 in OnabotA-injected animals when compared with the control group. In OnabotA+PHE treated animals mean signal intensity for Bcl-xL, Bax and Caspase 3 immunoreactions was identical to that of the control animals.

Conclusions

These results support the hypothesis that OnabotA activates apoptotic pathways in the rat prostate through a mechanism that involves sympathetic outflow impairment.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2490/12/1/prepub

Title: Expression of apoptosis-regulating genes in the rat prostate following botulinum toxin type a injection
Authors: Tiago Gorgal
Ana Charrua
João F Silva
António Avelino
Paulo Dinis
Francisco Cruz
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: BMC Urology / Issue 1/2012
Electronic ISSN: 1471-2490
DOI: https://doi.org/10.1186/1471-2490-12-1

At a glance: The STEP trials

Springer Medicine

Expression of apoptosis-regulating genes in the rat prostate following botulinum toxin type a injection

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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