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Open Access 01-12-2010 | Research article

The effect of three hemostatic agents on early bone healing in an animal model

Authors: Jonathan K Armstrong, Bo Han, Kenrick Kuwahara, Zhi Yang, Clara E Magyar, Sarah M Dry, Elisa Atti, Sotirios Tetradis, Timothy C Fisher

Published in: BMC Surgery | Issue 1/2010

Abstract

Background

Resorbable bone hemostasis materials, oxidized regenerated cellulose (ORC) and microfibrillar collagen (MFC), remain at the site of application for up to 8 weeks and may impair osteogenesis. Our experimental study compared the effect of a water-soluble alkylene oxide copolymer (AOC) to ORC and MFC versus no hemostatic material on early bone healing.

Methods

Two circular 2.7 mm non-critical defects were made in each tibia of 12 rabbits. Sufficient AOC, ORC or MFC was applied to achieve hemostasis, and effectiveness recorded. An autologous blood clot was applied to control defects. Rabbits were sacrificed at 17 days, tibiae excised and fixed. Bone healing was quantitatively measured by micro-computed tomography (micro-CT) expressed as fractional bone volume, and qualitatively assessed by histological examination of decalcified sections.

Results

Hemostasis was immediate after application of MFC and AOC, after 1-2 minutes with ORC, and >5 minutes for control. At 17 days post-surgery, micro-CT analysis showed near-complete healing in control and AOC groups, partial healing in the ORC group and minimal healing in the MFC group. Fractional bone volume was 8 fold greater in the control and AOC groups than in the MFC group (0.42 ± 0.06, 0.40 ± 0.03 vs 0.05 ± 0.01, P < 0.001) and over 1.5-fold greater than in the ORC group (0.25 ± 0.03, P < 0.05). By histology, MFC remained at the application site with minimal healing at the defect margins and early fibrotic tissue within the defect. ORC-treated defects showed partial healing but with early fibrotic tissue in the marrow space. Conversely, control and AOC-treated defects demonstrated newly formed woven bone rich in cellular activity with no evidence of AOC remaining at the application site.

Conclusions

Early healing appeared to be impaired by the presence of MFC and impeded by the presence of ORC. In contrast, AOC did not inhibit bone healing and suggest that AOC may be a better bone hemostatic material for procedures where bony fusion is critical and immediate hemostasis required.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2482/10/37/prepub

Title: The effect of three hemostatic agents on early bone healing in an animal model
Authors: Jonathan K Armstrong
Bo Han
Kenrick Kuwahara
Zhi Yang
Clara E Magyar
Sarah M Dry
Elisa Atti
Sotirios Tetradis
Timothy C Fisher
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: BMC Surgery / Issue 1/2010
Electronic ISSN: 1471-2482
DOI: https://doi.org/10.1186/1471-2482-10-37

At a glance: The STEP trials

Springer Medicine

The effect of three hemostatic agents on early bone healing in an animal model

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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