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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2007

Open Access 01-12-2007 | Research article

Intrinsic differentiation potential of adolescent human tendon tissue: an in-vitro cell differentiation study

Authors: Marieke de Mos, Wendy JLM Koevoet, Holger Jahr, Monique MA Verstegen, Marinus P Heijboer, Nicole Kops, Johannes PTM van Leeuwen, Harrie Weinans, Jan AN Verhaar, Gerjo JVM van Osch

Published in: BMC Musculoskeletal Disorders | Issue 1/2007

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Abstract

Background

Tendinosis lesions show an increase of glycosaminoglycan amount, calcifications, and lipid accumulation. Therefore, altered cellular differentiation might play a role in the etiology of tendinosis. This study investigates whether adolescent human tendon tissue contains a population of cells with intrinsic differentiation potential.

Methods

Cells derived from adolescent non-degenerative hamstring tendons were characterized by immunohistochemistry and FACS-analysis. Cells were cultured for 21 days in osteogenic, adipogenic, and chondrogenic medium and phenotypical evaluation was carried out by immunohistochemical and qPCR analysis. The results were compared with the results of similar experiments on adult bone marrow-derived stromal cells (BMSCs).

Results

Tendon-derived cells stained D7-FIB (fibroblast-marker) positive, but α-SMA (marker for smooth muscle cells and pericytes) negative. Tendon-derived cells were 99% negative for CD34 (endothelial cell marker), and 73% positive for CD105 (mesenchymal progenitor-cell marker). In adipogenic medium, intracellular lipid vacuoles were visible and tendon-derived fibroblasts showed upregulation of adipogenic markers FABP4 (fatty-acid binding protein 4) and PPARG (peroxisome proliferative activated receptor γ). In chondrogenic medium, some cells stained positive for collagen 2 and tendon-derived fibroblasts showed upregulation of collagen 2 and collagen 10. In osteogenic medium Von Kossa staining showed calcium deposition although osteogenic markers remained unaltered. Tendon-derived cells and BMCSs behaved largely comparable, although some distinct differences were present between the two cell populations.

Conclusion

This study suggests that our population of explanted human tendon cells has an intrinsic differentiation potential. These results support the hypothesis that there might be a role for altered tendon-cell differentiation in the pathophysiology of tendinosis.
Appendix
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Metadata
Title
Intrinsic differentiation potential of adolescent human tendon tissue: an in-vitro cell differentiation study
Authors
Marieke de Mos
Wendy JLM Koevoet
Holger Jahr
Monique MA Verstegen
Marinus P Heijboer
Nicole Kops
Johannes PTM van Leeuwen
Harrie Weinans
Jan AN Verhaar
Gerjo JVM van Osch
Publication date
01-12-2007
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2007
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/1471-2474-8-16

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