Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2014

Open Access 01-12-2014 | Research article

Acute cell viability and nitric oxide release in lateral menisci following closed-joint knee injury in a lapine model of post-traumatic osteoarthritis

Authors: Megan L Killian, Roger C Haut, Tammy L Haut Donahue

Published in: BMC Musculoskeletal Disorders | Issue 1/2014

Login to get access

Abstract

Background

Traumatic impaction is known to cause acute cell death and macroscopic damage to cartilage and menisci in vitro. The purpose of this study was to investigate cell viability and macroscopic damage of the medial and lateral menisci using an in situ model of traumatic loading. Furthermore, the release of nitric oxide from meniscus, synovium, cartilage, and subchondral bone was also documented.

Methods

The left limbs of five rabbits were subjected to tibiofemoral impaction resulting in anterior cruciate ligament (ACL) rupture and meniscal damage. Meniscal tear morphology was assessed immediately after trauma and cell viability of the lateral and medial menisci was assessed 24 hrs post-injury. Nitric oxide (NO) released from joint tissues to the media was assayed at 12 and 24 hrs post injury.

Results

ACL and meniscal tearing resulted from the traumatic closed joint impact. A significant decrease in cell viability was observed in the lateral menisci following traumatic impaction compared to the medial menisci and control limbs. While NO release was greater in the impacted joints, this difference was not statistically significant.

Conclusion

This is the first study to investigate acute meniscal viability following an in situ traumatic loading event that results in rupture of the ACL. The change in cell viability of the lateral menisci may play a role in the advancement of joint degeneration following traumatic knee joint injury.
Appendix
Available only for authorised users
Literature
1.
Frobell RB, Le Graverand MP, Buck R, Roos EM, Roos HP, Tamez-Pena J, Totterman S, Lohmander LS: The acutely ACL injured knee assessed by MRI: changes in joint fluid, bone marrow lesions, and cartilage during the first year. Osteoarthr Cartil. 2009, 17: 161-167.CrossRefPubMed
2.
Johnson DL, Urban WP, Caborn DN, Vanarthos WJ, Carlson CS: Articular cartilage changes seen with magnetic resonance imaging-detected bone bruises associated with acute anterior cruciate ligament rupture. Am J Sports Med. 1998, 26: 409-414.PubMed
3.
Isaac DI, Meyer EG, Haut RC: Development of a traumatic anterior cruciate ligament and meniscal rupture model with a pilot in vivo study. J Biomech Eng. 2010, 132: 064501-CrossRefPubMed
4.
Killian ML, Isaac DI, Haut RC, Déjardin LM, Leetun D, Haut Donahue TL: Traumatic anterior cruciate ligament tear and its implications on meniscal degradation: a preliminary novel lapine osteoarthritis model. J Surg Res. 2010, 164: 234-241.CrossRefPubMed
5.
Ewers BJ, Dvoracek-Driksna D, Orth MW, Haut RC: The extent of matrix damage and chondrocyte death in mechanically traumatized articular cartilage explants depends on rate of loading. J Orthop Res. 2001, 19: 779-784.CrossRefPubMed
6.
Kisiday JD, Vanderploeg EJ, McIlwraith CW, Grodzinsky AJ, Frisbie DD: Mechanical injury of explants from the articulating surface of the inner meniscus. Arch Biochem Biophys. 2010, 494: 138-144.CrossRefPubMed
7.
Hashimoto S, Takahashi K, Amiel D, Coutts RD, Lotz M: Chondrocyte apoptosis and nitric oxide production during experimentally induced osteoarthritis. Arthritis Rheum. 1998, 41: 1266-1274.CrossRefPubMed
8.
Cillero-Pastor B, Martin MA, Arenas J, López-Armada MJ, Blanco FJ: Effect of nitric oxide on mitochondrial activity of human synovial cells. BMC Musculoskelet Disord. 2011, 12: 42-CrossRefPubMedPubMedCentral
9.
Gupta T, Zielinska B, McHenry J, Kadmiel M, Haut Donahue TL: IL-1 and iNOS gene expression and NO synthesis in the superior region of meniscal explants are dependent on the magnitude of compressive strains. Osteoarthr Cartil. 2008, 16: 1213-1219.CrossRefPubMed
10.
Fermor B, Weinberg JB, Pisetsky DS, Misukonis MA, Banes AJ, Guilak F: The effects of static and intermittent compression on nitric oxide production in articular cartilage explants. J Orthop Res. 2001, 19: 729-737.CrossRefPubMed
11.
Hashimoto S, Takahashi K, Ochs RL, Coutts RD, Amiel D, Lotz M: Nitric oxide production and apoptosis in cells of the meniscus during experimental osteoarthritis. Arthritis Rheum. 1999, 42: 2123-2131.CrossRefPubMed
12.
Kobayashi K, Mishima H, Harwood F, Hashimoto S, Toyoguchi T, Goomer R, Moriya H, Amiel D: The suppressive effect of hyaluronan on nitric oxide production and cell apoptosis in the central region of meniscus following partial meniscectomy. Iowa Orthop J. 2002, 22: 39-41.PubMedPubMedCentral
13.
Isaac DI, Meyer EG, Haut RC: Chondrocyte damage and contact pressures following impact on the rabbit tibiofemoral joint. J Biomech Eng. 2008, 130: 41018-CrossRef
14.
Selinummi J, Seppälä J, Yli-Harja O, Puhakka JA: Software for quantification of labeled bacteria from digital microscope images by automated image analysis. Biotechniques. 2005, 39: 859-863.CrossRefPubMed
15.
Hufeland M, Schünke M, Grodzinsky AJ, Imgenberg J, Kurz B: Response of mature meniscal tissue to a single injurious compression and interleukin-1 in vitro. Osteoarthritis Cartilage. 2013, 21 (1): 209-216.CrossRefPubMed
16.
Natoli RM, Athanasiou KA: P188 reduces cell death and IGF-I reduces GAG release following single-impact loading of articular cartilage. J Biomech Eng. 2008, 130: 41012-CrossRef
17.
Serbest G, Horwitz J, Jost M, Barbee K: Mechanisms of cell death and neuroprotection by poloxamer 188 after mechanical trauma. FASEB J. 2006, 20: 308-310.PubMed
18.
Phillips DM, Haut RC: The use of a non-ionic surfactant (P188) to save chondrocytes from necrosis following impact loading of chondral explants. J Orthop Res. 2004, 22: 1135-1142.CrossRefPubMed
19.
Isaac DI, Golenberg N, Haut RC: Acute repair of chondrocytes in the rabbit tibiofemoral joint following blunt impact using P188 surfactant and a preliminary investigation of its long-term efficacy. J Orthop Res. 2010, 28: 553-558.PubMed
20.
Zhao D, Banks SA, D’Lima DD, Colwell CW, Fregly BJ: In vivo medial and lateral tibial loads during dynamic and high flexion activities. J Orthop Res. 2007, 25: 593-602.CrossRefPubMed
21.
Mundermann A, Dyrby CO, D’Lima DD, Colwell CW, Andriacchi TP: In vivo knee loading characteristics during activities of daily living as measured by an instrumented total knee replacement. J Orthop Res. 2008, 26: 1167-1172.CrossRefPubMed
22.
Isaac DI, Meyer EG, Kopke KS, Haut RC: Chronic changes in the rabbit tibial plateau following blunt trauma to the tibiofemoral joint. J Biomech. 2010, 43: 1682-1688.CrossRefPubMed
23.
Levine JW, Kiapour AM, Quatman CE, Wordeman SC, Goel VK, Hewett TE, Demetropoulos CK: Clinically relevant injury patterns after an anterior cruciate ligament injury provide insight into injury mechanisms. Am J Sports Med. 2013, 41 (2): 385-395.CrossRefPubMed
24.
Yeow C-H, Kong C-Y, Lee PV-S, Goh JC-H: Correlation of axial impact forces with knee joint forces and kinematics during simulated ski-landing. J Sports Sci. 2011, 29: 1143-1151.CrossRefPubMed
25.
Metadata
Title
Acute cell viability and nitric oxide release in lateral menisci following closed-joint knee injury in a lapine model of post-traumatic osteoarthritis
Authors
Megan L Killian
Roger C Haut
Tammy L Haut Donahue
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2014
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/1471-2474-15-297

Other articles of this Issue 1/2014

BMC Musculoskeletal Disorders 1/2014 Go to the issue

Research article

Levels of neuropeptide Y in synovial fluid relate to pain in patients with knee osteoarthritis

Research article

Thoracic pedicle classification determined by inner cortical width of pedicles on computed tomography images: its clinical significance for posterior vertebral column resection to treat rigid and severe spinal deformities—a retrospective review of cases

Research article

Can diagnostic injections predict the outcome in foot and ankle arthrodesis?

Study protocol

CASINO: Surgical or Nonsurgical Treatment for cervical radiculopathy, a randomised controlled trial

Research article

Radiographic analysis of the restoration of hip joint center following open reduction and internal fixation of acetabular fractures: a retrospective cohort study

Research article

Impact of patient-accessible electronic medical records in rheumatology: use, satisfaction and effects on empowerment among patients