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BMC Cancer
Published in: BMC Cancer 1/2013

Open Access 01-12-2013 | Research article

ID3 contributes to cerebrospinal fluid seeding and poor prognosis in medulloblastoma

Authors: Ji Hoon Phi, Seung Ah Choi, Sang-Hee Lim, Joongyub Lee, Kyu-Chang Wang, Sung-Hye Park, Seung-Ki Kim

Published in: BMC Cancer | Issue 1/2013

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Abstract

Background

The inhibitor of differentiation (ID) genes have been implicated as promoters of tumor progression and metastasis in many human cancers. The current study investigated the expression and functional roles of ID genes in seeding and prognosis of medulloblastoma.

Methods

ID gene expression was screened in human medulloblastoma tissues. Knockdown of ID3 gene was performed in medulloblastoma cells in vitro. The expression of metastasis-related genes after ID3 knockdown was assessed. The effect of ID3 knockdown on tumor seeding was observed in an animal model in vivo. The survival of medulloblastoma patients was plotted according to the ID3 expression levels.

Results

Significantly higher ID3 expression was observed in medulloblastoma with cerebrospinal fluid seeding than tumors without seeding. Knockdown of ID3 decreased proliferation, increased apoptosis, and suppressed the migration of D283 medulloblastoma cells in vitro. In a seeding model of medulloblastoma, ID3 knockdown in vivo with shRNA inhibited the growth of primary tumors, prevented the development of leptomeningeal seeding, and prolonged animal survival. High ID3 expression was associated with shorter survival of medulloblastoma patients, especially in Group 4 medulloblastomas.

Conclusions

High ID3 expression is associated with medullolbastoma seeding and is a poor prognostic factor, especially in patients with Group 4 tumors. ID3 may represent the metastatic/ aggressive phenotype of a subgroup of medulloblastoma.
Appendix
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Metadata
Title
ID3 contributes to cerebrospinal fluid seeding and poor prognosis in medulloblastoma
Authors
Ji Hoon Phi
Seung Ah Choi
Sang-Hee Lim
Joongyub Lee
Kyu-Chang Wang
Sung-Hye Park
Seung-Ki Kim
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2013
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-13-291

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