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BMC Cancer
Published in: BMC Cancer 1/2011

Open Access 01-12-2011 | Research article

Myofibrillogenesis regulator 1 (MR-1) is a novel biomarker and potential therapeutic target for human ovarian cancer

Authors: Renquan Lu, Min Sun, Jingjing Feng, Xiang Gao, Lin Guo

Published in: BMC Cancer | Issue 1/2011

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Abstract

Background

Myofibrillogenesis regulator 1 (MR-1) is overexpressed in human cancer cells and plays an essential role in cancer cell growth. However, the significance of MR-1 in human ovarian cancer has not yet been explored. The aim of this study was to examine whether MR-1 is a predictor of ovarian cancer and its value as a therapeutic target in ovarian cancer patients.

Methods

Reverse-transcription polymerase chain reaction (PCR) and quantitative real-time PCR were used to detect MR-1 mRNA levels in tissue samples from 26 ovarian cancer patients and 25 controls with benign ovarian disease. Anti-MR-1 polyclonal antibodies were prepared, tested by ELISA and western blotting, and then used for immunohistochemical analysis of the tissue samples. Adhesion and invasion of 292T cells was also examined after transfection of a pMX-MR-1 plasmid. Knockdown of MR-1 expression was achieved after stable transfection of SKOV3 cells with a short hairpin DNA pGPU6/GFP/Neo plasmid against the MR-1 gene. In addition, SKOV3 cells were treated with paclitaxel and carboplatin, and a potential role for MR-1 as a therapeutic target was evaluated.

Results

MR-1 was overexpressed in ovarian cancer tissues and SKOV3 cells. 293T cells overexpressed MR-1, and cellular spread and invasion were enhanced after transfection of the pMX-MR-1 plasmid, suggesting that MR-1 is critical for ovarian cancer cell growth. Knockdown of MR-1 expression inhibited cell adhesion and invasion, and treatment with anti-cancer drugs decreased its expression in cancer cells. Taken together, these results provide the first evidence of the cellular and molecular mechanisms by which MR-1 might serve as a novel biological marker and potential therapeutic target for ovarian cancer.

Conclusions

MR-1 may be a biomarker for diagnosis of ovarian cancer. It may also be useful for monitoring of the effects of anti-cancer therapies. Further studies are needed to clarify whether MR-1 is an early diagnostic marker for ovarian cancer and a possible therapeutic target.
Appendix
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Literature
1.
American Cancer Society: Cancer Facts & Figures 2009. 2009, Atlanta: American Cancer Society, 4-
2.
Liu B, Nash J, Runowicz C, Swede H, Stevens R, Li Z: Ovarian cancer immunotherapy: opportunities, progresses and challenges. J Hematol Oncol. 2010, 3: 7-10.1186/1756-8722-3-7.CrossRefPubMedPubMedCentral
3.
Fazal F, Gu LZ, Ihnatovych I, Han YJ, Hu WY, Antic N, Carreira F, Blomquist JF, Hope TJ, Ucker DS, Lanerolle PD: Inhibiting myosin light chain kinase induces apoptosis in vitro and in vivo. Mol Cell Biol. 2005, 25: 6259-6266. 10.1128/MCB.25.14.6259-6266.2005.CrossRefPubMedPubMedCentral
4.
Roovers K, Assoian RK: Effects of Rho kinase and actin stress fibers on sustained extracellular signal-regulated kinase activity and activation of G (1) phase cyclin-dependent kinases. Mol Cell Biol. 2003, 23: 4283-4294. 10.1128/MCB.23.12.4283-4294.2003.CrossRefPubMedPubMedCentral
5.
Gutjahr MC, Rossy J, sNiggli V: Role of Rho, Rac, and Rho-kinase in phosphorylation of myosin light chain, development of polarity, and spontaneous migration of Walker 256 carcinosarcoma cells. Exp Cell Res. 2005, 308: 422-438. 10.1016/j.yexcr.2005.05.001.CrossRefPubMed
6.
Ren KH, Jin HX, Bian CJ, He HW, Liu X, Zhang SH, Wang YG, Shao RG: MR-1 modulates proliferation and migration of human hepatoma HEPG2 cells through MLC2/FAK/AKT signaling pathway. J Bio Chem. 2008, 283: 35598-35605. 10.1074/jbc.M802253200.CrossRef
7.
Li TB, Liu XH, Feng S, Hu Y, Yang WX, Han Y, Wang YG, Gong LM: Characterization of MR-1, a novel myofibrillogenesis regulator in human muscle. Acta Biochimica et Biophysica Sinica. 2004, 36: 405-411. 10.1093/abbs/36.6.405.CrossRefPubMed
8.
Rainier S, Thomas D, Tokarz D, Ming L, Bui M, Plein E, Zhao X, Lemons R, Albin R, Delaney C, Alvarado D, Fink JK: Myofibrillogenesis regulator 1 gene mutations cause paroxysmal dystonia choreoathetosis. Arch Neurol. 2004, 61: 1025-1029. 10.1001/archneur.61.7.1025.CrossRefPubMed
9.
Ghezzi D, Viscomi C, Ferlini A, Gualandi F, Mereghetti P, DeGrandis D, Zeviani M: Paroxysmal non-kinesigenic dyskinesia is caused by mutations of the MR-1 mitochondrial targeting sequence. Human Molecular Genetics. 2009, 18: 1058-1064. 10.1093/hmg/ddn441.CrossRefPubMed
10.
Chen DH, Matsushita M, Rainier S, Meaney B, Tisch L, Feleke A, Wolff J, Lipe H, Fink J, Bird TD, Raskind WH: Presence of alanine-to-valine substitutions in myofibrillogenesis regulator 1 in paroxysmal nonkinesigenic dyskinesia. Arch Neurol. 2005, 62: 597-600. 10.1001/archneur.62.4.597.CrossRefPubMed
11.
Li HL, She ZG, Li TB, Wang AB, Yang QL, Wei YS, Wang YG, Liu DP: Overexpression of Myofibrillogenesis Regulator-1 Aggravates Cardiac Hypertrophy Induced by Angiotensin II in Mice. Hypertension. 2007, 49: 1399-1408. 10.1161/HYPERTENSIONAHA.106.085399.CrossRefPubMed
12.
Clemens MJ, Bushel LM, Jeffrey IW, Pain VM, Morley SJ: Translation initiation factor modifications and the regulation of protein synthesis in apoptotic cells. Cell Death Differ. 2000, 7: 603-615. 10.1038/sj.cdd.4400695.CrossRefPubMed
13.
Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. 2001, New York: Cold Spring Harbor Laboratory Press, 3
14.
Yamazaki D, Kurisu S, Takenawa T: Regulation of cancer cell motility through actin reorganization. Cancer Sci. 2005, 96: 379-386. 10.1111/j.1349-7006.2005.00062.x.CrossRefPubMed
15.
Lu RQ, Guo L, Hu J: Studies on the value of human epididymis protein 4 in patients with ovarian cancer. Ch J Clin Lab. 2009, 32: 1379-1383.
16.
McFadyen MC, Cruickshank ME, Miller ID, McLeod HL, Melvin WT, Haites NE, Parkin D, Murray GI: Cytochrome P450 over-expression in primary and metastatic ovarian cancer. Br J Cancer. 2001, 85: 242-246. 10.1054/bjoc.2001.1907.CrossRefPubMedPubMedCentral
Metadata
Title
Myofibrillogenesis regulator 1 (MR-1) is a novel biomarker and potential therapeutic target for human ovarian cancer
Authors
Renquan Lu
Min Sun
Jingjing Feng
Xiang Gao
Lin Guo
Publication date
01-12-2011
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2011
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-11-270

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