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Open Access 01-12-2011 | Research article

Platelet factor-4 and its p17-70 peptide inhibit myeloma proliferation and angiogenesis in vivo

Authors: Longjiang Yang, Juan Du, Jian Hou, Hua Jiang, Jianfeng Zou

Published in: BMC Cancer | Issue 1/2011

Abstract

Background

Angiogenesis plays an important role in the development of multiple myeloma (MM). The interaction between MM cells and the bone marrow microenvironment stimulates the proliferation and migration of endothelial progenitor cells (EPCs). Vascular endothelial growth factor (VEGF) contributes to the formation of new blood vessels by actively recruiting circulating EPCs. The production of proangiogenic and antiangiogenic factors is also dysregulated in MM. Platelet factor 4 (PF4) is a potent angiostatic cytokine that inhibits angiogenesis and tumor growth in several animal models.

Methods

In this study, we stably transfected human myeloma cell lines with the PF4 gene or the sequence encoding its more potent p17-70 peptide and investigated the effects of PF4 and p17-70 on angiogenesis and tumor growth in vitro and in a SCID-rab myeloma model.

Results

PF4 and p17-70 significantly attenuated VEGF production, both in vitro and in vivo. In a migration study using a Transwell system, PF4 or p17-70 markedly suppressed the migration of co-cultured human endothelial progenitor cells. PF4 or p17-70 also caused a significant reduction in microvessel densities in myeloma xenografts and markedly reduced the tumor volume in the SCID mice. Kaplan-Meier analysis demonstrated that PF4 and p17-70 significantly extended the overall survival of SCID mice bearing human myeloma xenografts.

Conclusions

Our findings indicate that PF4 or p17-70 could be valuable in combating multiple myeloma by disrupting tumor angiogenesis.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/11/261/prepub

Title: Platelet factor-4 and its p17-70 peptide inhibit myeloma proliferation and angiogenesis in vivo
Authors: Longjiang Yang
Juan Du
Jian Hou
Hua Jiang
Jianfeng Zou
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2011
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-11-261

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Abstract

Background

Methods

Results

Conclusions

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