Published in:
Open Access
01-12-2010 | Research article
Phosphorylated Smad2 in Advanced Stage Gastric Carcinoma
Authors:
Osamu Shinto, Masakazu Yashiro, Takahiro Toyokawa, Takafumi Nishii, Ryoji Kaizaki, Taro Matsuzaki, Satoru Noda, Naoshi Kubo, Hiroaki Tanaka, Yosuke Doi, Masaichi Ohira, Kazuya Muguruma, Tetsuji Sawada, Kosei Hirakawa
Published in:
BMC Cancer
|
Issue 1/2010
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Abstract
Background
Transforming growth factor β (TGFβ) receptor signaling is closely associated with the invasion ability of gastric cancer cells. Although Smad signal is a critical integrator of TGFβ receptor signaling transduction systems, not much is known about the role of Smad2 expression in gastric carcinoma. The aim of the current study is to clarify the role of phosphorylated Smad2 (p-Smad2) in gastric adenocarcinomas at advanced stages.
Methods
Immunohistochemical staining with anti-p-Smad2 was performed on paraffin-embedded specimens from 135 patients with advanced gastric adenocarcinomas. We also evaluated the relationship between the expression levels of p-Smad2 and clinicopathologic characteristics of patients with gastric adenocarcinomas.
Results
The p-Smad2 expression level was high in 63 (47%) of 135 gastric carcinomas. The p-Smad2 expression level was significantly higher in diffuse type carcinoma (p = 0.007), tumours with peritoneal metastasis (p = 0.017), and tumours with lymph node metastasis (p = 0.047). The prognosis for p-Smad2-high patients was significantly (p = 0.035, log-rank) poorer than that of p-Smad2-low patients, while a multivariate analysis revealed that p-Smad2 expression was not an independence prognostic factor.
Conclusion
The expression of p-Smad2 is associated with malignant phenotype and poor prognosis in patients with advanced gastric carcinoma.