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Open Access 01-12-2010 | Research article

Expression of CIAPIN1 in human colorectal cancer and its correlation with prognosis

Authors: Hai Shi, Yi Zhou, Heliang Liu, Changsheng Chen, Shujun Li, Nanlin Li, Xiaohua Li, Xi Zhang, Hongwei Zhang, Weizhong Wang, Qingchuan Zhao

Published in: BMC Cancer | Issue 1/2010

Abstract

Background

The cytokine-induced anti-apoptotic molecule (CIAPIN1) had been found to be a differentially-expressed gene involved in a variety of cancers, and it was also considered as a candidate tumour suppressor gene in gastric cancer, renal cancer and liver cancer. However, studies on the role of CIAPIN1 in colorectal cancer were still unavailable. The aim of this study was to determine the prognostic impact of CIAPIN1 in 273 colorectal cancer (CRC) samples and to investigate the CIAPIN1 expression in CRC cell lines after inducing differentiation.

Methods

Immunohistochemical analysis was performed to detect the expression of CIAPIN1 in CRC samples from 273 patients. The relationship between CIAPIN1 expression and patients' characteristics (gender, age, location of cancer, UICC stage, local recurrence and tumour grade factors) was evaluated. In addition, these patients were followed up for five consecutive years to investigate the relationship between CIAPIN1 expression and the prognosis of CRC. We induced the differentiation of the CRC cell lines HT29 and SW480, in order to detect the expression of CIAPIN1 in the process of CRC cells differentiation.

Results

Results indicated that CIAPIN1 was mainly expressed in the cytoplasm and nucleus, and that its expression level in cancer samples was significantly lower than in normal tissues. The Wilcoxon-Mann-Whitney test showed a significant difference in the differential expression of CIAPIN1 in patients with different T and UICC stages, and tumour grade (P = 0.0393, 0.0297 and 0.0397, respectively). The Kaplan-Meier survival analysis demonstrated that the survival time of CRC patients with high expression of CIAPIN1 was longer than those with low expression during the 5-year follow up period (P = 0.0002). COX regression analysis indicated that low expression of CIAPIN1, cancer stage of > pT1, distant organ metastasis (pM₁), regional lymph node metastasis (> pN₁) and local recurrence (yes) were independent, poor prognostic factors of CRC (P = 0.012, P = 0.032, P < 0.001, P < 0.001, P < 0.001 respectively). Both Western blotting and RT-PCR showed that CIAPIN1 expression was increased with the degree of differentiation of HT29 and SW480 cells.

Conclusions

CIAPIN1 played an important role in the differentiation of CRC cells, and the differential expression of CIAPIN1 in CRC was closely related to prognosis.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/10/477/prepub

Title: Expression of CIAPIN1 in human colorectal cancer and its correlation with prognosis
Authors: Hai Shi
Yi Zhou
Heliang Liu
Changsheng Chen
Shujun Li
Nanlin Li
Xiaohua Li
Xi Zhang
Hongwei Zhang
Weizhong Wang
Qingchuan Zhao
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2010
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-10-477

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Abstract

Background

Methods

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Conclusions

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