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Clinical Phytoscience
Published in: Clinical Phytoscience 1/2017

Open Access 01-01-2017 | Original contribution

Antioxidative and neuroprotective effects of Leea macrophylla methanol root extracts on diazepam-induced memory impairment in amnesic Wistar albino rat

Authors: Sakia Ferdousy, Md Atiar Rahman, Md Mamun Al-Amin, Jannatul Aklima, J. M. Kamirul Hasan Chowdhury

Published in: Clinical Phytoscience | Issue 1/2017

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Abstract

Background

A neurological disorder is becoming one of the major health problems worldwide. Synthetic drugs for neurological disorders often produce unwanted effects while traditional plant- derived drugs offer a unique strategy in combating the neurological diseases. This study investigated how the antioxidative properties of Leea macrophylla root extracts integratively help as neuroprotective and antiamnesic in animal model.

Methods

Leea macrophylla root methanol extract (LM) was assessed by open field test, hole cross test, elevated plus maze test and thiopental sodium induced sleeping time test for screening neuropharmacological effect on Wistar albino rats using the LM at 100 & 200 mg/kg body weight. Antiamnesic effect of LM was studied on diazepam induced anterograde amnesia in Wistar albino rats. Morris water maze test was performed to evaluate whether the learning and memory of rats are impaired by amnesia produced by diazepam. The antioxidative enzymes superoxide dismutase, catalase, glutathione peroxidase and oxidative biomarkers malondialdehyde, nitric oxide, advanced oxidation protein product from the hippocampus of decapitated animals were measured to assess the antioxidative potential of the extract.

Results

LM was found to show significant (p <0.05) reduction in locomotor activity and increase in the duration of sleeping of animals. Morris water maze test showed that animals treated with LM significantly (p <0.05) decreased the mean escape latency compared to animals in diazepam group. LM was also found to reduce the content of malondialdehyde, nitric oxide and advanced oxidation protein product and to increase the activities of superoxide dismutase, catalase and glutathione peroxidase in hippocampus.

Conclusion

Our studies suggest that antioxidative capacity of LM in hippocampus may augment the antiamnesic effects in anterograde amnesia.
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Metadata
Title
Antioxidative and neuroprotective effects of Leea macrophylla methanol root extracts on diazepam-induced memory impairment in amnesic Wistar albino rat
Authors
Sakia Ferdousy
Md Atiar Rahman
Md Mamun Al-Amin
Jannatul Aklima
J. M. Kamirul Hasan Chowdhury
Publication date
01-01-2017
Publisher
Springer Berlin Heidelberg
Published in
Clinical Phytoscience / Issue 1/2017
Electronic ISSN: 2199-1197
DOI
https://doi.org/10.1186/s40816-016-0031-6

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