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International Journal of Implant Dentistry

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Open Access 01-12-2021 | Research

Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum

Authors: Chu-nan Zhang, Lin-yi Zhou, Shu-jiao Qian, Ying-xin Gu, Jun-yu Shi, Hong-chang Lai

Published in: International Journal of Implant Dentistry | Issue 1/2021

Abstract

Objectives

This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms.

Materials and methods

Titanium plates were divided into two groups: (1) no coating (Ti, control), (2) Tantalum-coated titanium (Ta-coated Ti). All samples were characterized by scanning electronic microscopy, surface roughness, and hydrophilicity. Fibroblasts’ performance were analyzed by attached cell number at 1 h, 4 h, and 24 h, morphology at 1 h and 4 h, viability at 1 day, 3 days, 5 days, and 7 days, recovery after wounding at 6 h, 12 h, and 24 h. RT-PCR, western blot were applied to detect attachment-related genes’ expression and protein synthesis at 4 h and 24 h. Student’s t test was used for statistical analysis.

Results

Tantalum-coated titanium demonstrates a layer of homogeneously distributed nano-grains with mean diameter of 25.98 (± 14.75) nm. It was found that after tantalum deposition, human gingival fibroblasts (HGFs) adhesion, viability, proliferation, and migration were promoted in comparison to the control group. An upregulated level of Integrin β1 and FAK signaling was also detected, which might be the underlying mechanism.

Conclusion

In the present study, adhesion, viability, proliferation, migration of human gingival fibroblasts are promoted on tantalum-coated titanium, upregulated integrin β1 and FAK might contribute to its superior performance, indicating tantalum coating can be applied in transmucosal part of dental implant.

Clinical significance

Tantalum deposition on titanium surfaces can promote human gingival fibroblast adhesion, accordingly forming a well-organized soft tissue sealing and may contribute to a successful osseointegration.

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Title: Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum
Authors: Chu-nan Zhang
Lin-yi Zhou
Shu-jiao Qian
Ying-xin Gu
Jun-yu Shi
Hong-chang Lai
Publication date: 01-12-2021
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Implant Dentistry / Issue 1/2021
Electronic ISSN: 2198-4034
DOI: https://doi.org/10.1186/s40729-021-00316-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical significance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical significance

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